HPLC & UHPLC Analytical Columns

particle size: 1.9µm, 3µm, or 5µm, spherical
pore size: 140Å
carbon load: 8%
endcap: yes
pH range: 2.5 to 8
temperature limit: 80°C

Pinnacle® DB Biphenyl is a unique reversed phase material that displays both increased retention and selectivity for aromatic and/or unsaturated compounds when compared to conventional alkyl and phenyl phases. Highly base-deactivated spherical silica manufactured by Restek. An excellent choice for the analysis of steroids, tetracyclines, drug metabolites, and other compounds that contain some degree of unsaturation. Part of the Restek USLC™ column set, which offers the widest range of reversed phase selectivity available!

particle size: 1.9µm
pore size: 140Å
endcap: yes
pH range: 2.5 to 8
temperature limit: 80°C

An intrinsically base-deactivated (IBD) phase, containing a polar group within, or intrinsic to, the hydrocarbon bonded phase. Unique selectivity and a high level of base deactivation, while reducing or eliminating the need for mobile phase additives. Part of the Restek USLC™ column set, which offers the widest range of reversed phase selectivity available!

particle size: 1.9µm
pore size: 140Å
endcap: yes
pH range: 2.5 to 8
temperature limit: 80°C

Specifically designed to resolve complex mixtures of polycyclic aromatic hydrocarbons.

particle size: 1.9µm, 3µm, or 5µm, spherical
pore size: 140Å
carbon load: 6%
endcap: yes
pH range: 2.5 to 8
temperature limit: 80°C

Pinnacle® DB PFP Propyl, a unique pentafluorophenyl phase with a propyl spacer, uses a highly base-deactivated spherical silica manufactured by Restek. This highly base-deactivated packing exhibits excellent peak shapes for a wide range of compounds, including nucleosides, nucleotides, and halogenated compounds. Part of the Restek USLC™ column set, which offers the widest range of reversed phase selectivity available!

particle size: 5µm
pore size: 300Å
carbon load: 6.7%
endcap: yes
pH range: 2.5 to 8
temperature limit: 80°C

Highly base-deactivated wide pore packing that exhibits excellent peak shape for a wide range of compounds; ideal for large molecule and biomolecule assays. Highly retentive and selective phase for aromatic and unsaturated compounds, with increased retention, relative to phenyl phases.

particle size: 5µm, spherical
pore size: 300Å
carbon load: 5%
endcap: yes
pH range: 2.5 to 8
temperature limit: 80°C

A pentafluorophenyl phase with a propyl spacer. Highly retentive for basic analytes. Highly base-deactivated wide pore packing that exhibits excellent peak shape for a wide range of compounds, including nucleosides, nucleotides, and halogenated compounds.

particle size: 5µm, spherical
pore size: 300Å
carbon load: 3.5%
endcap: yes
pH range: 2.5 to 8
temperature limit: 80°C

Highly base-deactivated wide pore packing that exhibits excellent peak shape for a wide range of compounds. Less retention in reversed phase assays than Viva C18 or Viva C8.

particle size: 5µm, spherical
pore size: 300Å
carbon load: 5%
endcap: yes
pH range: 2.5 to 8
temperature limit: 80°C

Highly base-deactivated wide pore packing that exhibits excellent peak shape for a wide range of compounds. Less retention in reversed phase assays than Viva C18.

particle size: 3µm or 5µm, spherical
pore size: 300Å
carbon load: 9%
endcap: yes
pH range: 2.5 to 8
temperature limit: 80°C

Highly base-deactivated wide pore packing that exhibits excellent peak shape for a wide range of compounds. Excellent general-purpose column for analyzing large molecules and biomolecules.

particle size: 5µm, spherical
pore size: 300Å
pH range: 2.5 to 8
temperature limit: 80°C

Highly base-deactivated wide pore packing that exhibits excellent peak shape for a wide range of compounds in normal phase separations.

particle size: 1.9µm, 3µm, or 5µm, spherical
pore size: 140Å
endcap: no
pH range: 2.5 to 8
temperature limit: 80°C

Highly base-deactivated spherical silica manufactured by Restek. Useful for normal phase separations. Replaces Hypersil® BDS and Pinnacle® Amine.

particle size: 1.9µm or 5µm, spherical
pore size: 140Å
carbon load: 4%
endcap: yes
pH range: 2.5 to 8
temperature limit: 80°C

Highly base-deactivated spherical silica manufactured by Restek. Cyano bonding. Suitable for analyses of a wide range of compounds, from acidic through slightly basic. Also useful for confirmation of analyses on a C18 or C8 column. Can be used in normal phase or reversed phase mode of separation. Replaces Hypersil® BDS Cyano and Pinnacle® Cyano Amine.

particle size: 1.9µm, 3µm, or 5µm, spherical
pore size: 140Å
carbon load: 6%
endcap: yes
pH range: 2.5 to 8
temperature limit: 80°C

Highly base-deactivated spherical silica manufactured by Restek. Monomeric C8 bonding. Similar to Pinnacle® DB C18, but the shorter alkyl chain provides less hydrophobic retention. Less retention can be useful for reducing analysis time, if resolution is adequate. Replaces Hypersil® BDS C8 and Pinnacle® C8 Amine.

particle size: 1.9µm, 3µm, or 5µm, spherical
pore size: 140Å
carbon load: 11%
endcap: yes
pH range: 2.5 to 8
temperature limit: 80°C

Highly base-deactivated spherical silica manufactured by Restek. Monomeric C18 bonding. Hydrophobic C18 phase suitable for analyses of a wide range of compounds, from acidic through slightly basic. Replaces Hypersil® BDS C18 and Pinnacle® ODS Amine.

particle size: 3µm or 5µm, spherical
pore size: 110Å
endcap: no
pH range: 2.5 to 8
temperature limit: 80°C

Good general purpose packing for normal phase separations. Moderate surface area. Replaces Hypersil® and Pinnacle® Silica.

particle size: 3µm or 5µm, spherical
pore size: 110Å
carbon load: 4%
endcap: fully endcapped
pH range: 2.5 to 8
temperature limit: 80°C

Can be used in either reversed phase or normal phase mode. More rugged than bare silica for normal phase applications. Replaces Hypersil® Cyano and Pinnacle® CN.

particle size: 3µm or 5µm, spherical
pore size: 110Å
carbon load: 7%
endcap: fully endcapped
pH range: 2.5 to 8
temperature limit: 80°C

Reliable performance and symmetric peaks for neutral to acidic compounds. Provides shorter retention times for hydrophobic compounds, compared to C18 phases. Replaces Hypersil® C8 and Pinnacle® C8.

particle size: 3µm or 5µm, spherical
pore size: 110Å
carbon load: 13%
endcap: fully endcapped
pH range: 2.5 to 8
temperature limit: 80°C

Excellent choice as a general purpose C18 column. Intermediate carbon loading and surface area, suitable for a wide range of acidic to neutral hydrophobic compounds. Replaces Hypersil® ODS and Pinnacle® C18.

particle size: 3µm or 5µm, spherical
pore size: 110Å
carbon load: 2%
endcap: no
pH range: 2.5 to 8
temperature limit: 80°C

HPLC analysis using an amino-based stationary phase is the most popular technique for routine analyses of simple sugars, using isocratic elution (e.g., acetonitrile:water, 75:25) and a refractive index detector (RID) or an evaporative light scattering detector (ELSD). The Pinnacle® II Amino column is ideal for mono- and disaccharide analyses. Replaces Hypersil® Amino and Pinnacle® Amino.

• USLC™ method development toolbox contains all 4 USLC™ stationary phases in 1 convenient package.
• Available for UHPLC (1.9 μm) and HPLC (3 or 5 μm) in 50, 100, or 150 mm lengths.
• Included selection guide makes it even easier to pick the right column the first time.
• Learn more about USLC™ columns at www.restek.com/uslc

particle size: 3µm or 5µm, spherical
pore size: 100Å
carbon load: 17%
endcap: fully endcapped
pH range: 2.5 to 8
temperature limit: 80°C

Ultra Aromax is a unique reversed phase material that exhibits superior retention and selectivity for aromatic and/or unsaturated compounds, compared to conventional alkyl and phenyl phases. This column is a great alternative to our Biphenyl phase when increased retention is required. A very suitable choice for analysis of steroids, tetracyclines, drug metabolites, and other compounds that contain some degree of unsaturation.

particle size: 3µm or 5µm, spherical
pore size: 100Å
carbon load: 15%
endcap: fully endcapped
pH range: 2.5 to 8
temperature limit: 80°C

A pentafluorophenyl phase with a propyl spacer. Highly retentive for basic analytes. An excellent phase for separating nucleosides, nucleotides, purines, pyrimidines, and halogenated compounds. Part of the Restek USLC™ column set, which offers the widest range of reversed phase selectivity available!

particle size: 3µm or 5µm, spherical
pore size: 100Å
carbon load: 15%
endcap: fully endcapped
pH range: 2.5 to 8
temperature limit: 80°C

A unique reversed phase material that exhibits both increased retention and selectivity for aromatic and/or unsaturated compounds, compared to conventional alkyl and phenyl phases. This is a great alternative to a C18 column when alternative selectivity is desired. An excellent choice for the analysis of steroids, tetracyclines, drug metabolites, and other compounds that contain some degree of unsaturation. Part of the Restek USLC™ column set, which offers the widest range of reversed phase selectivity available!

particle size: 4µm, spherical
pore size: 110Å
endcap: fully endcapped
pH range: 2.5 to 8
temperature limit: 80°C

Developed specifically for challenging analyses of polycyclic aromatic hydrocarbons. The Pinnacle® II PAH stationary phase incorporates a proprietary C18 bonding that enables unique shape selectivity to resolve to baseline all 16 PAHs listed in US EPA Method 610. Every lot of Pinnacle® II PAH bonded phase material is tested to ensure baseline resolution of the Method 610 PAHs, using a simple water/acetonitrile mobile phase gradient.

particle size: 5µm
pore size: 60Å
endcap: yes
pH range: 2.5 to 8
temperature limit: 80°C

This highly retentive, highly selective phase, unique to Restek, was developed specifically for the analysis of aldehydes and ketones as DNPH derivatives. Allure® AK is a reversed phase HPLC material that has the unique ability to separate all thirteen carbonyl compounds specified in California Air Resources Board (CARB) Method # 1004, using a simple acetonitrile/water gradient, in less than 15 minutes. Other columns require long analysis times or the use of tetrahydrofuran.

particle size: 5µm, spherical
pore size: 60Å
carbon load: 23%
endcap: yes
pH range: 2.5 to 8
temperature limit: 80°C

Highly retentive and selective for aromatic and unsaturated compounds. Increased retention and selectivity, compared to phenyl phases. Excellent selectivity for steroids, tetracyclines, explosives, and other unsaturated compounds.

particle size: 3µm or 5µm, spherical
pore size: 100Å
pH range: 2.5 to 8
temperature limit: 80°C

A retentive, high-purity, base deactivated reversed phase packing. Ideal for the analysis of paraquat and diquat or other quaternary amines when used with Ultra Quat Mobile Phase Additive (cat.# 32462).

Technical Article: Fast, Sensitive LC/MS/MS Analysis of Paraquat and Diquat

particle size: 5µm spherical
pore size: 60Å
endcap: no
pH range: 2.5 to 8
temperature limit: 80°C

Highly retentive and selective phase for separating polar analytes, including polar acidic compounds. Compatible with highly aqueous (up to 100%) mobile phases. Highly base deactivated. An excellent choice when analyzing a wide range of compounds, as in LC/MS screening methods.

particle size: 5µm, spherical
pore size: 60Å
endcap: no
pH range: 2.5 to 8
temperature limit: 80°C

Allure® Organic Acids columns provide enhanced retention and selectivity for polar organic acids, allowing the separation to be performed on a single 30cm column. An Allure® Organic Acids column effectively resolves key organic acids such as tartaric and quinic acids, using the chromatographic conditions specified in AOAC method 986.13. Retention is stable and reproducible, even with the 100% aqueous mobile phase specified in the AOAC method.

particle size: 3µm or 5µm, spherical
pore size: 100Å
pH range: 2.5 to 8
temperature limit: 80°C

Restek chemists developed the Ultra Carbamate column specifically for carbamates analysis. The unique packing separates 10 target carbamates in just over 10 minutes. The column is compatible with fluorescence or LC/MS detection.* An Ultra Carbamate column can process as many as 3 to 4 samples per hour, versus less than 2 samples per hour on a general-purpose C18 column. In addition to increased sample throughput, this much faster analysis will significantly reduce solvent usage—and the costs of disposing of solvent waste.

particle size: 5µm, spherical
pore size: 60Å
carbon load: 12%
endcap: fully endcapped
pH range: 2.5 to 8
temperature limit: 80°C

Highly retentive propyl cyano phase. Excellent choice for basic compounds and for analytes containing amine group functionality.

particle size: 3µm or 5µm, spherical
pore size: 100Å
endcap: no
pH range: 2.5 to 8
temperature limit: 80°C

High surface area, Type B silica packing.

particle size: 3µm or 5µm, spherical
pore size: 100Å
carbon load: 12%
endcap: no
pH range: 2.5 to 8
temperature limit: 80°C

An intrinsically base-deactivated (IBD) phase, containing a polar group within, or intrinsic to, the hydrocarbon bonded phase. Unique selectivity and a high level of base deactivation, while reducing or eliminating the need for mobile phase additives. Part of the Restek USLC™ column set, which offers the widest range of reversed phase selectivity available!

particle size: 3µm or 5µm, spherical
pore size: 100Å
carbon load: 8%
endcap: fully endcapped
pH range: 2.5 to 8
temperature limit: 80°C

High-purity cyano phase with few silanol sites. Often a better choice than C18 phases for basic pharmaceuticals, especially regarding peak shape and selectivity. Cyano phases are more rugged than bare silica for normal phase analyses because they are less sensitive to small amounts of water in the mobile phase.

particle size: 3µm or 5µm, spherical
pore size: 100Å
carbon load: 15%
endcap: no
pH range: 2.5 to 8
temperature limit: 80°C

Highly retentive and selective for reversed phase separations of polar analytes. Highly base-deactivated. Compatible with highly aqueous (up to 100%) mobile phases. Part of the Restek USLC™ column set, which offers the widest range of reversed phase selectivity available!

particle size: 3µm or 5µm, spherical
pore size: 100Å
carbon load: 20%
endcap: fully endcapped
pH range: 2.5 to 8
temperature limit: 80°C

A retentive, high-purity packing that exhibits excellent peak shape for a wide range of compounds. Excellent general-purpose reversed phase column.

particle size: 3µm or 5µm, spherical
pore size: 100Å
carbon load: 12%
endcap: fully endcapped
pH range: 2.5 to 8
temperature limit: 80°C

A retentive, high-purity, base-deactivated reversed phase packing that exhibits excellent peak shape for a wide range of compounds. Less retention for neutral, hydrophobic compounds, compared to the Ultra C18 column.

particle size: 3µm or 5µm, spherical
pore size: 100Å
carbon load: 9%
endcap: fully endcapped
pH range: 2.5 to 8
temperature limit: 80°C

Exceptionally stable C4 packing, with high bonding coverage and base deactivation. Less retention than C18 or C8 phases.

particle size: 3µm or 5µm, spherical
pore size: 100Å
carbon load: 5%
pH range: 2.5 to 8
temperature limit: 80°C

Exceptionally stable C1 phase. Least retentive reversed phase hydrocarbon packing.

particle size: 3µm or 5µm, spherical
pore size: 100Å
carbon load: 2%
encap: no
pH range: 2.5 to 8
temperature limit: 80°C

Recommended for normal phase analyses of mono- and disaccharides, or similar compounds.

particle size: 5µm, spherical
pore size: 60Å
endcap: no
pH range: 2.5 to 8
temperature limit: 80°C

Highly retentive phase for normal phase separations. Very high surface area, Type B silica packing.

particle size: 5µm, spherical
pore size: 60Å
carbon load: 17%
endcap: fully endcapped
pH range: 2.5 to 8
temperature limit: 80°C

A pentafluorophenyl phase with a propyl spacer. Highly retentive for basic analytes. An excellent phase for separating nucleosides, nucleotides, purines, pyrimidines, halogenated compounds, β-blockers, and tricyclic antidepressants.

particle size: 5µm, spherical
pore size: 60Å
carbon load: 27%
endcap: fully endcapped
pH range: 2.5 to 8
temperature limit: 80°C

Most retentive of our alkyl stationary phases due to large surface area of the base silica and high-density bondings. Provides excellent peak shapes for a wide range of compounds.

particle size: 1.9µm, 3µm, or 5µm, spherical
pore size: 140Å
carbon load: 6%
pH range: 2.5 to 8
temperature limit: 80°C

Highly selective phase for polar analytes. Compatible with highly aqueous (up to 100%) mobile phases. Silica manufactured by Restek. Part of the Restek USLC™ column set, which offers the widest range of reversed phase selectivity available!

particle size: 5µm, spherical
pore size: 110Å
endcap: yes
pH range: 2.5 to 8
temperature limit: 80°C

The Pinnacle® II Biphenyl phase offers alternate selectivity to straight-chain hydrocarbon phases, and enhanced selectivity and retention for unsaturated compounds, compared to traditional phenyl phases. An excellent confirmation column for explosive compounds, as in EPA method 8330.

Fast, efficient separations in an economical cartridge format. The design allows rapid substitution of one cartridge for another. The holder is available with either standard fittings or with a universal PEEK tip fitting. The universal fitting gives the user the option of threading the column directly into the detector inlet, reducing system dwell volume. The cartridges are 30mm long, with either 2.1mm or 4.0mm ID. The 2.1mm and 4.0mm ID cartridges have the same outside diameter, so the holder will accept either.

Use these kits to simplify HPLC method development. If they don't fit your needs, call for a kit customized to your specifications.

Each kit includes three 50 x 2.1mm columns.

Use these kits to simplify HPLC method development. If they don't fit your needs, call for a kit customized to your specifications.

Each kit includes three 150 x 4.6mm columns.

Use this kit to simplify HPLC method development.

Kit includes four 50 x 4.6mm, 5µm columns: 1 Pinnacle DB C18 (USP L1), 1 Allure PFP Propyl, 1 Ultra Aqueous C18 (USP L1), 1 Ultra IBD