HPLC & UHPLC Analytical Columns
Small Molecule

particle size: 1.9 µm, 3 µm, or 5 µm, spherical
pore size: 140 Å
carbon load: 8%
end-cap: yes
pH range: 2.5 to 8
temperature limit: 80 °C
USP phase: L11
phase category: phenyl
ligand type: unique Biphenyl

particle size: 3µm or 5µm, spherical
pore size: 110Å
carbon load: 2%
endcap: no
pH range: 2.5 to 8
temperature limit: 80°C

HPLC analysis using an amino-based stationary phase is the most popular technique for routine analyses of simple sugars, using isocratic elution (e.g., acetonitrile:water, 75:25) and a refractive index detector (RID) or an evaporative light scattering detector (ELSD). The Pinnacle® II Amino column is ideal for mono- and disaccharide analyses. Replaces Hypersil® Amino and Pinnacle® Amino.

particle size: 3µm or 5µm, spherical
pore size: 110Å
carbon load: 13%
endcap: fully endcapped
pH range: 2.5 to 8
temperature limit: 80°C

Excellent choice as a general purpose C18 column. Intermediate carbon loading and surface area, suitable for a wide range of acidic to neutral hydrophobic compounds. Replaces Hypersil® ODS and Pinnacle® C18.

particle size: 3µm or 5µm, spherical
pore size: 110Å
carbon load: 7%
endcap: fully endcapped
pH range: 2.5 to 8
temperature limit: 80°C

Reliable performance and symmetric peaks for neutral to acidic compounds. Provides shorter retention times for hydrophobic compounds, compared to C18 phases. Replaces Hypersil® C8 and Pinnacle® C8.

particle size: 3µm or 5µm, spherical
pore size: 110Å
carbon load: 4%
endcap: fully endcapped
pH range: 2.5 to 8
temperature limit: 80°C

Can be used in either reversed phase or normal phase mode. More rugged than bare silica for normal phase applications. Replaces Hypersil® Cyano and Pinnacle® CN.

particle size: 1.9 µm, 3 µm, or 5 µm, spherical
pore size: 140 Å
carbon load: 6%
end-cap: yes
pH range: 2.5 to 8
temperature limit: 80 °C
USP phase code: L43
phase category: fluorophenyl propyl
ligand type: pentafluorophenyl propyl

particle size: 3µm or 5µm, spherical
pore size: 110Å
endcap: no
pH range: 2.5 to 8
temperature limit: 80°C

Good general purpose packing for normal phase separations. Moderate surface area. Replaces Hypersil® and Pinnacle® Silica.

particle size: 1.9 µm, spherical
pore size: 140 Å
end-cap: no
pH range: 2.5 to 8
temperature limit: 80 °C

particle size: 1.9 µm, 3 µm, or 5 µm, spherical
pore size: 140 Å
carbon load: 11%
end-cap: yes
pH range: 2.5 to 8
temperature limit: 80 °C
USP phase code: L1
phase category: C18, octadecylsilane
ligand type: monomeric C18

particle size: 1.9 µm, spherical
pore size: 140 Å
end-cap: no
pH range: 2.5 to 8
temperature limit: 80 °C
USP phase code: L68
phase category: polar-embedded alkyl
ligand type: proprietary polar functional embedded alkyl

particle size: 1.9 µm or 5 µm, spherical
pore size: 140 Å
carbon load: 4%
end-cap: yes
pH range: 2.5 to 8
temperature limit: 80 °C
USP phase code: L10
phase category: cyano
ligand type: cyanopropylsilane

particle size: 1.9 µm, 3 µm, or 5 µm, spherical
pore size: 140 Å
end-cap: no
pH range: 2.5 to 8
temperature limit: 80 °C
USP phase code: L3
phase category: bare silica
ligand type: none

particle size: 5µm, spherical
pore size: 60Å
carbon load: 12%
endcap: fully endcapped
pH range: 2.5 to 8
temperature limit: 80°C

Highly retentive propyl cyano phase. Excellent choice for basic compounds and for analytes containing amine group functionality.

particle size: 5 µm, spherical
pore size: 60 Å
end-cap: no
pH range: 2.5 to 8
temperature limit: 80 °C

particle size: 5µm spherical
pore size: 60Å
endcap: no
pH range: 2.5 to 8
temperature limit: 80°C

Highly retentive and selective phase for separating polar analytes, including polar acidic compounds. Compatible with highly aqueous (up to 100%) mobile phases. Highly base deactivated. An excellent choice when analyzing a wide range of compounds, as in LC/MS screening methods.

particle size: 5µm, spherical
pore size: 60Å
carbon load: 27%
endcap: fully endcapped
pH range: 2.5 to 8
temperature limit: 80°C

Most retentive of our alkyl stationary phases due to large surface area of the base silica and high-density bondings. Provides excellent peak shapes for a wide range of compounds.

particle size: 3 µm or 5 µm, spherical
pore size: 100 Å
pH range: 2.5 to 8
temperature limit: 80 °C

A retentive, high-purity, base deactivated reversed phase packing. Ideal for the analysis of paraquat and diquat or other quaternary amines.

Technical Article: Fast, Sensitive LC/MS/MS Analysis of Paraquat and Diquat

particle size: 5µm, spherical
pore size: 60Å
carbon load: 17%
endcap: fully endcapped
pH range: 2.5 to 8
temperature limit: 80°C

A pentafluorophenyl phase with a propyl spacer. Highly retentive for basic analytes. An excellent phase for separating nucleosides, nucleotides, purines, pyrimidines, halogenated compounds, β-blockers, and tricyclic antidepressants.

particle size: 5µm, spherical
pore size: 60Å
endcap: no
pH range: 2.5 to 8
temperature limit: 80°C

Highly retentive phase for normal phase separations. Very high surface area, Type B silica packing.

particle size: 3 µm or 5 µm, spherical
pore size: 100 Å
carbon load: 2%
end-cap: no
pH range: 2.5 to 8
temperature limit: 80 °C
USP phase code: L8
phase category: amino
ligand type: aminopropylsilane

particle size: 5µm, spherical
pore size: 60Å
carbon load: 23%
endcap: yes
pH range: 2.5 to 8
temperature limit: 80°C

Highly retentive and selective for aromatic and unsaturated compounds. Increased retention and selectivity, compared to phenyl phases. Excellent selectivity for steroids, tetracyclines, explosives, and other unsaturated compounds.

particle size: 3 µm or 5 µm, spherical
pore size: 100 Å
carbon load: 5%
pH range: 2.5 to 8
temperature limit: 80 °C

particle size: 5 µm, spherical
pore size: 60 Å
end-cap: yes
pH range: 2.5 to 8
temperature limit: 80 °C

particle size: 3 µm or 5 µm, spherical
pore size: 100 Å
carbon load: 9%
end-cap: yes
pH range: 2.5 to 8
temperature limit: 80 °C

particle size: 4 µm, spherical
pore size: 110 Å
end-cap: no
pH range: 2.5 to 8
temperature limit: 80 °C

particle size: 3 µm or 5 µm, spherical
pore size: 100 Å
carbon load: 12%
end-cap: yes
pH range: 2.5 to 8
temperature limit: 80 °C
USP phase code: L7
phase category: C8, octylsilane
ligand type: monomeric C8

Our C8 is a conventional monomeric octylsilane column offering a shorter alkyl chain to provide less hydrophobic retention and improved basic peak shape over a traditional C18 phase. Like our C18, this general-purpose Restek C8 is suitable for a wide range of compounds from acidic through slightly basic.

particle size: 3 µm or 5 µm, spherical
pore size: 100 Å
carbon load: 15%
end-cap: yes
pH range: 2.5 to 8
temperature limit: 80 °C
USP phase: L11
phase category: phenyl
ligand type: unique Biphenyl

particle size: 3 µm or 5 µm, spherical
pore size: 100 Å
carbon load: 20%
end-cap: yes
pH range: 2.5 to 8
temperature limit: 80 °C
USP phase code: L1
phase category: C18, octadecylsilane
ligand type: monomeric C18

The general-purpose Restek C18 is a conventional monomeric octadecylsilane column suitable for analyses of a wide range of compounds from acidic through slightly basic.

particle size: 3 µm or 5 µm, spherical
pore size: 100 Å
carbon load: 11%
end-cap: yes
pH range: 2.5 to 8
temperature limit: 80 °C
USP phase code: L43
phase category: fluorophenyl propyl
ligand type: pentafluorophenyl propyl

particle size: 3 µm or 5 µm, spherical
pore size: 100 Å
carbon load: 15%
end-cap: no
pH range: 2.5 to 8
temperature limit: 80 °C
USP phase code: L1
phase category: modified C18
ligand type: proprietary polar modified and functionally bonded C18

particle size: 3 µm or 5 µm, spherical
pore size: 100 Å
carbon load: 17%
end-cap: yes
pH range: 2.5 to 8
temperature limit: 80 °C
USP phase code: L11
phase category: phenyl
ligand type: proprietary phenyl ligand

Ultra Aromax is a unique reversed-phase material that exhibits extreme retention and selectivity for aromatic and/or unsaturated compounds. This column is a great alternative to our Biphenyl phase when increased retention is required, and it’s an excellent choice for gradient LC-MS analyses when conventional columns are not giving adequate retention or selectivity.

particle size: 3 µm or 5 µm, spherical
pore size: 100 Å
carbon load: 8%
end-cap: yes
pH range: 2.5 to 8
temperature limit: 80 °C
USP phase code: L10
phase category: cyano
ligand type: cyanopropylsilane

• Ultra Selective Liquid Chromatography (USLC®) method development toolbox contains all four USLC® stationary phases in one convenient package.
• Available for UHPLC (1.9 μm) and HPLC (3 or 5 μm) in 50, 100, or 150 mm lengths.
• Included selection guide makes it even easier to pick the right column the first time.
• Learn more about USLC® columns at www.restek.com/uslc

particle size: 3 µm or 5 µm, spherical
pore size: 100 Å
carbon load: 12%
end-cap: no
pH range: 2.5 to 8
temperature limit: 80 °C
USP phase code: L68
phase category: polar-embedded alkyl
ligand type: proprietary polar functional embedded alkyl

particle size: 3 µm or 5 µm, spherical
pore size: 100 Å
end-cap: no
pH range: 2.5 to 8
temperature limit: 80 °C
USP phase code: L3
phase category: bare silica
ligand type: none

particle size: 1.9 µm, 3 µm, or 5 µm, spherical
pore size: 140 Å
carbon load: 6%
pH range: 2.5 to 8
temperature limit: 80 °C
USP phase code: L1
phase category: modified C18
ligand type: proprietary polar modified and functionally bonded C18

Use this kit to simplify HPLC method development.

Kit includes four 50 x 4.6mm, 5µm columns: 1 Pinnacle DB C18 (USP L1), 1 Allure PFP Propyl, 1 Ultra Aqueous C18 (USP L1), 1 Ultra IBD

Use these kits to simplify HPLC method development. If they don't fit your needs, call for a kit customized to your specifications.

Each kit includes three 150 x 4.6mm columns.

Use these kits to simplify HPLC method development. If they don't fit your needs, call for a kit customized to your specifications.

Each kit includes three 50 x 2.1mm columns.

particle size: 5µm, spherical
pore size: 110Å
endcap: yes
pH range: 2.5 to 8
temperature limit: 80°C

The Pinnacle® II Biphenyl phase offers alternate selectivity to straight-chain hydrocarbon phases, and enhanced selectivity and retention for unsaturated compounds, compared to traditional phenyl phases. An excellent confirmation column for explosive compounds, as in EPA method 8330.

Fast, efficient separations in an economical cartridge format. The design allows rapid substitution of one cartridge for another. The holder is available with either standard fittings or with a universal PEEK tip fitting. The universal fitting gives the user the option of threading the column directly into the detector inlet, reducing system dwell volume. The cartridges are 30mm long, with either 2.1mm or 4.0mm ID. The 2.1mm and 4.0mm ID cartridges have the same outside diameter, so the holder will accept either.