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LC Columns Physical Characteristics Chart

LC Columns Physical Characteristics Chart

UHPLC Columns

Restek Column
End Cap?
Pore Size (Å)
Carbon load (%)
Applications
Chromatographic Properties
Similar Phases
USP Code
Y
140
6
Applications similar to Pinnacle DB C18, but with less hydrophobic retention. Less retention can be useful for shortening analysis time, if resolution is adequate.
Highly base-deactivated spherical silica manufactured by Restek. Monomeric C8 bonding. Similar to Pinnacle DB C18, but the shorter alkyl chain provides less hydrophobic retention.
Hypersil BDS C8, Spherisorb C8
L7
Y
140
11
Hydrophobic C18 phase suitable for analyses of a wide range of compounds, from acidic through slightly basic.
Highly base-deactivated spherical silica manufactured by Restek. Monomeric C18 bonding.
Hypersil BDS C18, Zorbax Eclipse XDB-C18, Spherisorb ODS
L1
140
6
Ideal for applications that require highly aqueous mobile phases, such as organic acids and water-soluble vitamins.
Highly selective phase for polar analytes. Compatible with highly aqueous (up to 100%) mobile phases. Silica manufactured by Restek.
Aquasil C18, AQUA C18, Hypersil Gold AQ, YMC ODS-Aq
L1
Y
140
8
Excellent choice for the analysis of steroids, tetracyclines, drug metabolites, and other compounds that contain some degree of unsaturation.
Highly base-deactivated spherical silica manufactured by Restek. Unique reversed phase material that displays both increased retention and selectivity for aromatic and/or unsaturated compounds when compared to conventional alkyl and phenyl phases.
Unique
L11
Y
140
A polar group assists in deactivating surface silanols and contributes to unique separation selectivities for acids, bases, zwitterions, and other polar compounds.
One of a group of intrinsically base-deactivated (IBD) phases, with a polar group within, or intrinsic to, the alkyl bonded phase. Provides unique selectivity and high level of base deactivation while reducing or eliminating the need for mobile phase additives.
Unique
L68
Y
140
6
Exhibits excellent peak shapes for a wide range of compounds, including nucleosides, nucleotides, and halogenated compounds.
Highly base-deactivated spherical silica manufactured by Restek. Unique pentafluorophenyl phase with a propyl spacer.
Discovery HS F5
L43
Y
140
4
Suitable for a wide range of compounds, from acidic through slightly basic. Also useful for confirmation of analyses on a C18 or C8 column. Can be used in normal phase or reversed phase mode of separation.
Highly base-deactivated spherical silica manufactured by Restek. Cyano bonding.
Hypersil BDS Cyano, Spherisorb Cyano, Zorbax Eclipse XDB-CN
L10
140
Normal phase mode of separation.
Highly base-deactivated spherical silica manufactured by Restek.
L3
Y
140
Ideal for polycyclic aromatic hydrocarbons.
Specifically designed to resolve complex mixtures of polycyclic aromatic hydrocarbons.
Unique

HPLC Columns

Restek Column
End Cap?
Pore Size (Å)
Carbon load (%)
Applications
Chromatographic Properties
Similar Phases
USP Code
Y
90
7% (2.7 µm)

5% (5 µm)
Ideal for bioanalytical testing applications like drug and metabolite analyses. pH range 1.5-8.0
Increased retention for dipolar, unsaturated, or conjugated solutes. Enhanced selectivity when used with methanolic mobile phase. Ideal for increasing sensitivity and selectivity in LC-MS analyses.
Kinetex Biphenyl
L11
90
7% (2.7 µm)

5% (5 µm)
Ideal for high-throughput LC-MS/MS applications with minimal sample preparation. Well-balanced retention profile for better detection and integration of large, multiclass analyte lists. pH range 1.0-8.0
Well-balanced retention profile. Sterically protected and acid-resistant to withstand harsh, low-pH mobile phases. Ideal for use with sensitive detectors like mass spec.
Kinetex XB-C18, Ascentis Express Peptide ES-C18, Poroshell 120 SB-C18
L1
Y
90
7% (2.7 µm)

5% (5 µm)
A traditional end-capped C18 ideal for general-purpose use in reversed-phase chromatography. pH range (2–8) provides excellent data quality for many applications, matrices, and compounds.
Compatible with moderately acidic to neutral mobile phases (pH 2–8). Excellent data quality in food, environmental, bioanalytical, and other applications.
Kinetex C18, Poroshell EC-C18, Ascentis Express C18, Halo C18, Sunshell C18, Nucleoshell RP 18, Accucore C18
L1
100
5
Alternative selectivity to Ultra C18 or C8 columns, especially for polar analytes. Shortest chain alkyl phase available for reversed phase separations.
Exceptionally stable C1 packing resists hydrolysis, even under acidic mobile phase conditions. Least retentive reversed phase hydrocarbon packing.
Spherisorb C1
L13
Y
100
9
Ideal for peptides and small proteins.
Exceptionally stable C4 packing, with high bonding coverage and silanol base-deactivation. Exhibits shorter retention than C18 or C8 phases.
Supelcosil Butyl (C4), Delta-Pak C4
L26
Y
100
12
Selectivity and peak shape similar to Ultra C18, but less hydrophobic retention.
Very retentive, high-purity, base-deactivated reversed phase packing that exhibits excellent peak shape for a wide range of compounds.
Luna C8, Symmetry C8, Hypersil Gold C8
L7
Y
100
20
Ideal for anilines, barbiturates, carbonyls, fat-soluble vitamins, fatty acids, glycerides, phthalates, PTH amino acids, steroids, other acids.
A very retentive, high-purity phase that exhibits excellent peak shape for a wide range of compounds. Recommended as a general purpose reversed phase column.
Discovery C18, Symmetry C18, Hypersil Gold C18, Luna C18, Zorbax C18, Kromasil C18, LiChrospher RP-18, Inertsil ODS-2, Develosil C18
L1
N
100
15
Ideal for analyses that require >90% water in the mobile phase. Excellent for highly water soluble or poorly organic soluble compounds. Excellent for water-soluble vitamins and organic acids.
Highly retentive and selective for reversed phase separations of polar analytes. Highly base deactivated. Compatible with highly aqueous (up to 100%) mobile phases.
AQUA C18, Aquasil C18, Hypersil Gold AQ, YMC ODS-Aq
L1
Y
100
15
An excellent choice for the analysis of steroids, tetracyclines, drug metabolites, and other compounds that contain some degree of unsaturation.
A unique reversed phase material that exhibits both increased retention and selectivity for aromatic and/or unsaturated compounds, compared to conventional alkyl and phenyl phases.
Unique
L11
Y
100
17
A very suitable choice for analysis of steroids, tetracyclines, drug metabolites, and other compounds that contain some degree of unsaturation.
A unique reversed phase material that exhibits superior retention and selectivity for aromatic and/or unsaturated compounds, compared to conventional alkyl and phenyl phases. This column is a great alternative to our Biphenyl phase when increased retention is required.
Unique
L11
N
100
12
A polar group assists in deactivating surface silanols and contributes to unique separation selectivities for acids, bases, zwitterions, and other polar compounds.
One of a group of intrinsically base-deactivated (IBD) phases, with a polar group within, or intrinsic to, the alkyl bonded phase. Provides unique selectivity and high level of base deactivation while reducing or eliminating the need for mobile phase additives.
SymmetryShield, Discovery ABZ & ABZ+, Prism
L68
Y
100
15
Highly retentive for basic analytes. An excellent phase for separating nucleosides, nucleotides, purines, pyrimidines, and halogenated compounds.
A pentafluorophenyl phase with a propyl spacer.
Discovery HS F5
L43
Y
100
8
Excellent for basic pharmaceuticals, steroids (normal or reversed phase conditions), or other basic compounds.
High-purity cyano phase with reduced silanol activity. Often a better choice than C18 for basic pharmaceuticals. Cyano is the most stable bonded phase for normal phase mode.
Platinum CN, Develosil Cyano, Luna CN, Hypersil Gold CN
L10
N
100
2
Superior general purpose amino phase. Ideal for carbohydrates.
Recommended for normal phase analyses of mono- and disaccharides and other similar compounds. Can also serve as a weak anion exchanger, with aqueous buffers.
Platinum Amino, Develosil NH2
L8
100
Ideal for normal phase applications.
High purity, high surface area.
L3

Specialty Columns

Restek Column
End Cap?
Pore Size (Å)
Carbon load (%)
Applications
Chromatographic Properties
Similar Phases
USP Code
Y
60
Ideal for the analysis of aldehydes and ketones as DNPH derivatives.
Highly retentive, highly selective phase, developed specifically for the analysis of aldehydes and ketones as DNPH derivatives.
Unique
Y
60
12
Ideal for LC/MS of basic solutes. Excellent for basic pharmaceuticals or other amine-containing compounds.
Highly retentive phase for analytes containing amino functionality.
BetaMax Base, Maxsil CN
L10
N
60
Excellent resolution of challenging organic acids.
Single 30cm column performs equally to two C18 columns in series. (AOAC Method 986.13)
Unique
Y
110
Maximum resolution of polycyclic aromatic hydrocarbons.
Proprietary stationary phase; resolves 16 PAHs in US EPA Method 610. Silica manufactured by Restek.
Unique
100
Rapid analysis of carbamates.
Proprietary stationary phase can process up to twice as many samples per hour, compared to a conventional C18 phase.
Unique
100
Proprietary phase for the analysis of paraquat and diquat and other quaternary amines.
High purity silica.
Unique

Large Molecule Columns

Restek Column
End Cap?
Pore Size (Å)
Carbon load (%)
Applications
Chromatographic Properties
Similar Phases
USP Code
Y
300
3.5
Proteins and other higher molecular weight compounds. Less retentive than C18 and C8 phases.
Silica manufactured by Restek.
BioBasic 4, Symmetry 300 C4, Jupiter 300 C4, Synchropak C4, 208 TP C4
L26
Y
300
5
Proteins and other higher molecular weight compounds. Less retentive than C18 phase.
Silica manufactured by Restek.
BioBasic 8, Zorbax 300 OSB C8, Synchropak C8, 208 TP C8
L7
Y
300
9
Proteins and other higher molecular weight compounds.
Silica manufactured by Restek.
BioBasic 18, Symmetry 300 C18, Jupiter 300 C18, Zorbax 300 OSB C18, Synchropak C18, 208 TP C18
L1
Y
300
6.7
Exhibits excellent peak shape for a wide range of compounds; ideal for large molecule and biomolecule assays.
Silica manufactured by Restek.
Unique
L11
Y
300
5
Exhibits excellent peak shape for a wide range of compounds, including nucleosides, nucleotides, and halogenated compounds.
Silica manufactured by Restek.
Unique
L43
300
Normal phase applications for highly retained high molecular weight compounds.
Silica manufactured by Restek.
L3