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LC Columns Physical Characteristics Chart

LC Columns Physical Characteristics Chart

UHPLC Columns

Restek Column
End Cap?
Pore Size (Å)
Carbon Load (%)
Surface Area (m2/g)
pH Range
Applications
Chromatographic Properties
Similar Phases
USP Code
Y
90
7
1.8 μm: 125
1.5 to 8.0
Ideal for bioanalytical and forensic toxicology testing applications like therapeutic drug monitoring (TDM), drugs of abuse, and metabolite analyses with UHPLC-MS/MS.
Increased retention for dipolar, unsaturated, or conjugated compounds. Enhanced selectivity when used with methanolic mobile phase vs ACN. Provides increased peak capacity compared to fully porous particles for increased resolution of complex samples.
Kinetex Biphenyl
L11
N
90
7
1.8 μm: 125
1.0 to 8.0
Recommended for high-throughput UHPLC-MS/MS applications with acidic mobile phases. Ideal for large, multiclass analyte lists including pesticides, vitamins, and amino acids.
Well-balanced retention profile emphasizing dispersion and hydrophobic interaction. Sterically protected ligand resists acid hydrolysis to withstand harsh, low-pH mobile phases used in UHPLC-MS/MS.
Kinetex XB-C18, Cortecs C18+
L1
Y
90
9
1.8 μm: 125
2.0 to 8.0
The "go-to" phase for a variety of compound types provides excellent data quality for food safety and quality, environmental, bioanalytical, and other applications.
A traditional end-capped C18 ideal for general-purpose use in reversed-phase chromatography. Increased retention of hydrophobic compounds provides excellent data quality with methanol and ACN mobile phases.
Kinetex C18, Ascentis Express C18, Cortecs C18, Poroshell SB-C18, Halo-2 C18
L1
N
90
4
1.8 μm: 125
2.0 to 8.0
Capable of running in reversed phase or HILIC mode for compounds like vitamin D epimers not well resolved on traditional C18 phases. Ideal for charged bases such as taxane drugs and other amine-containing compounds.
Electron-withdrawing fluorine atoms provide increased retention for charged bases. Reliable and efficient with acidic mobile phases for increasing selectivity and sensitivity in UHPLC-MS/MS analyses running in reversed phase or HILIC mode.
Kinetex F5, Ascentis Express F5, Poroshell PFP, Halo-2 PFP
L43
Y
100
20
300
2.0 to 8.0
Highly hydrophobic retentive with a pH range of between 2.0 to 8.0 suitable for the analysis of a wide range of compounds.
Traditional end-capped C18 ideal for general purpose reversed-phase use. Scalable from 1.8 μm to 3 μm to for predictable method transfer from HPLC to UHPLC.
Discovery C18, Symmetry C18, Hypersil Gold C18, Luna C18, Kromasil C18, LiChrospher RP-18, Inertsil ODS-2, Develosil C18, Acquity UPLC HSS C18, Zorbax Eclipse XDB-C18, Eclipse Plus C18, Syncronis C18 (1.7 μm)Titan C18 (1.9 μm)
L1
Y
100
15
300
2.0 to 8.0
Ideal for bioanalytical testing applications like drug and metabolite analyses. Heightened selectivity and retention for compounds that are hard to resolve or elute early on C18 and other phenyl chemistries.
Increased retention for dipolar, unsaturated, or conjugated compounds. Enhanced selectivity when used with methanolic mobile phase vs ACN. Provides increased sensitivity and selectivity for LC-MS/MS analyses.
Unique
L11
N
100
10
300
2.0 to 8.0
Reversed-phase or HILIC applications for a variety of compounds. Ideal for charged bases and other amine-containing compounds like xanthines and nitrofurans.
Electron-withdrawing fluorine atoms provide increased retention for charged bases. Reliable and efficient with acidic mobile phases for increasing selectivity and sensitivity in LC-MS/MS analyses running in reversed-phase or HILIC mode
Discovery HS F5, Luna PFP(2), Pursuit PFP, Acquity UPLC HSS PFP, Hypersil Gold PFP, Titan PFP (1.9 μm)
L43
Y
140
6
150
2.5 to 8.0
Applications similar to Pinnacle DB C18, but with less hydrophobic retention. Less retention can be useful for shortening analysis time, if resolution is adequate.
Highly base-deactivated spherical silica manufactured by Restek. Monomeric C8 bonding. Similar to Pinnacle DB C18, but the shorter alkyl chain provides less hydrophobic retention.
Hypersil BDS C8, Spherisorb C8, Zorbax C8
L7
Y
140
11
150
2.5 to 8.0
Hydrophobic C18 phase suitable for analyses of a wide range of compounds, from acidic through slightly basic.
Highly base-deactivated spherical silica manufactured by Restek Monomeric C18 bonding.
Hypersil BDS C18, Spherisorb ODS, Zorbax ODS
L1
140
6
150
2.5 to 8.0
Ideal for applications that require highly aqueous mobile phases, such as organic acids and water-soluble vitamins.
Highly selective phase for polar analytes. Compatible with highly aqueous (up to 100%) mobile phases. Silica manufactured by Restek.
Aquasil C18, AQUA C18, Hypersil Gold AQ, YMC ODS-Aq
L1
Y
140
8
150
2.5 to 8.0
Excellent choice for the analysis of steroids, tetracyclines, drug metabolites, and other compounds that contain some degree of unsaturation.
Highly base-deactivated spherical silica manufactured by Restek. Unique reversed-phase material that displays both increased retention and selectivity for aromatic and/or unsaturated compounds when compared to conventional alkyl and phenyl phases.
Unique
L11
Y
140
150
2.5 to 8.0
A polar group assists in deactivating surface silanols and contributes to unique separation selectivities for acids, bases, zwitterions, and other polar compounds.
One of a group of intrinsically base-deactivated (IBD) phases, with a polar group within, or intrinsic to, the alkyl bonded phase. Provides unique selectivity and high level of base deactivation while reducing or eliminating the need for mobile phase additives.
Unique
L68
Y
140
6
150
2.5 to 8.0
Exhibits excellent peak shapes for a wide range of compounds, including nucleosides, nucleotides, and halogenated compounds.
Highly base-deactivated spherical silica manufactured by Restek. Unique pentafluorophenyl phase with a propyl spacer.
Discovery HS F5
L43
Y
140
4
150
2.5 to 8.0
Suitable for a wide range of compounds, from acidic through slightly basic. Also useful for confirmation of analyses on a C18 or C8 column. Can be used in normal phase or reversed-phase mode of separation.
Highly base-deactivated spherical silica manufactured by Restek.Cyano bonding.
Hypersil BDS Cyano, Spherisorb Cyano, Zorbax CN
L10
140
150
2.8 to 8.0
Normal phase mode of separation.
Highly base-deactivated spherical silica manufactured by Restek.
Zorbax Silica
L3
Y
140
150
2.5 to 8.0
Ideal for polycyclic aromatic hydrocarbons.
Specifically designed to resolve complex mixtures of polycyclic aromatic hydrocarbons.
Unique

HPLC Columns

Restek Column
End Cap?
Pore Size (Å)
Carbon Load (%)
Surface Area (m2/g)
pH Range
Applications
Chromatographic Properties
Similar Phases
USP Code
Y
100
20
300
2.5 to 8.0
General-purpose HPLC column for a variety of compounds from acidic to slightly basic. Ideal for high-volume workflows using USP or other compendial type methods.
A very retentive, high-purity ODS phase that exhibits excellent peak shape for a wide range of compounds.
Luna C18, Zorbax Eclipse XDB C18, Eclipse Plus C18, Hypersil Gold C18, Symmetry C18, Reliant
L1
Y
100
12
300
2.5 to 8.0
General-purpose HPLC column for a variety of compounds from acidic to slightly basic. Ideal for high-volume workflows using USP or other compendial-type methods.
The shorter alkyl chain results in less hydrophobic retention and improved basic peak shape over a traditional C18.
Luna C8, Zorbax Eclipse XDB-C8, Eclipse Plus C8, Hypersil Gold C8, Symmetry C8
L7
Y
100
15
300
2.0 to 8.0
General-purpose HPLC column ideal for high-volume workflows using USP or other compendial-type methods. Recommended for aromatic analytes and complex samples.
A phenyl ring attached to a 6-carbon chain provides alternate selectivity to straight-chain hydrocarbon phases. Analyte interaction profile includes both dispersion and aromatic polarizability.
Luna Phenyl-Hexyl, Zorbax Eclipse XDB- Phenyl, Eclipse Plus Phenyl-Hexyl, Hypersil Gold Phenyl
L11
Y
100
8
300
2.5 to 8.0
Recommended for assays where alternate selectivity or confirmation to a C8 or C18 is desired. Also an excellent choice for protonated bases. Ideal for high-volume workflows using USP or other compendial-type methods.
Cyanopropyl silane that can be used in normal phase, reversed-phase, or HILIC modes.
Luna CN, Zorbax Eclipse XDB-CN, Hypersil Cyano, XSelect CN
L10
100
300
2.5 to 8.0
Small nonpolar compounds in normal phase mode, polar compounds like water-soluble vitamins, steroids, and organic acids in HILIC mode.
Base-deactivated for normal phase or HILIC separations.
Luna Silica, Hypersil Gold Silica, Pursuit XRs-Si
L3
Y
90
7
(2.7 µm)

5
(5 µm)
2.7 μm: 130,

5 μm: 100
1.5 to 8.0
Ideal for bioanalytical testing applications like drug and metabolite analyses. Heightened selectivity and retention for compounds that are hard to resolve or elute early on C18 and other phenyl chemistries.
Increased retention for dipolar, unsaturated, or conjugated solutes. Enhanced selectivity when used with methanolic mobile phase. Ideal for increasing sensitivity and selectivity in LC-MS analyses.
Kinetex Biphenyl, Ascentis Express Biphenyl
L11
90
7
(2.7 µm)

5
(5 µm)
2.7 μm: 130,

5 μm: 100
1.0 to 8.0
Ideal for high-throughput LC-MS/MS applications with minimal sample preparation. Well-balanced retention profile for better detection and integration of large, multiclass analyte lists.
Well-balanced retention profile. Sterically protected and acid-resistant to withstand harsh, low-pH mobile phases. Ideal for use with sensitive detectors like mass spec.
Kinetex XB-C18, Ascentis Express Peptide ES-C18, Poroshell 120 SB-C18
L1
Y
90
7
(2.7 µm)

5
(5 µm)
2.7 μm: 130,

5 μm: 100
2.0 to 8.0
A traditional end-capped C18 ideal for general-purpose use in reversed-phase chromatography. pH range (2–8) provides excellent data quality for many applications, matrices, and compounds.
Compatible with moderately acidic to neutral mobile phases (pH 2–8). Excellent data quality in food, environmental, bioanalytical, and other applications.
Kinetex C18, Poroshell EC-C18, Ascentis Express C18, Halo C18, Sunshell C18, Nucleoshell RP 18, Accucore C18, Cortecs C18, ACE UltraCore Super C18, Accucore RP-MS
L1
N
90
4
(2.7 µm)

3
(5 µm)
2.7 μm: 130,

5 μm: 100
2.0 to 8.0
HILIC applications and compounds like vitamin D epimers not well resolved on traditional C18 phases. Ideal for charged bases such as taxane drugs and other amine-containing compounds.
Electron-withdrawing fluorine atoms provide increased retention for charged bases. Reliable and efficient with acidic mobile phases for increasing selectivity and sensitivity in LCMS/MS analyses.
Accucore PFP, Ascentis Express F5, Halo 2.7 PFP, Kinetex PFP, Nucleoshell PFP
L43
N
90
130
2.0 to 8.0
Ideal for small polar compounds, especially nitrogen-containing, which are protonated under high organic conditions.
Retention of small polar molecules without ion-pairing reagents.
Cortecs HILIC, Poroshell 120 HILIC, Kinetex HILIC, Ascentis Express HILIC, Halo HILIC, Accucore HILIC
L3
100
5
300
2.5 to 8.0
Alternative selectivity to Ultra C18 or C8 columns, especially for polar analytes. Shortest chain alkyl phase available for reversed-phase separations.
Exceptionally stable C1 packing resists hydrolysis, even under acidic mobile phase conditions. Least retentive reversed-phase hydrocarbon packing.
Spherisorb C1
L13
Y
100
9
300
2.5 to 8.0
Ideal for peptides and small proteins.
Exceptionally stable C4 packing, with high bonding coverage and silanol base deactivation. Exhibits shorter retention than C18 or C8 phases.
Supelcosil Butyl (C4), Delta-Pak C4
L26
Y
100
12
300
2.5 to 8.0
Selectivity and peak shape similar to Ultra C18, but less hydrophobic retention.
Very retentive, high-purity, base-deactivated reversed-phase packing that exhibits excellent peak shape for a wide range of compounds.
Luna C8, Symmetry C8, Hypersil Gold C8
L7
Y
100
20
300
2.5 to 8.0
Ideal for anilines, barbiturates, carbonyls, fat-soluble vitamins, fatty acids, glycerides, phthalates, PTH amino acids, steroids, other acids.
A very retentive, high-purity phase that exhibits excellent peak shape for a wide range of compounds. Recommended as a general purpose reversed-phase column.
Discovery C18, Symmetry C18, Hypersil Gold C18, Luna C18, Kromasil C18, LiChrospher RP-18, Inertsil ODS-2, Develosil C18, Zorbax Eclipse XDB-CB, Eclipse Plus C-18
L1
N
100
15
300
2.5 to 8.0
Ideal for analyses that require >90% water in the mobile phase. Excellent for highly water-soluble or poorly organic-soluble compounds. Excellent for water-soluble vitamins and organic acids.
Highly retentive and selective for reversed-phase separations of polar analytes. Highly base deactivated. Compatible with highly aqueous (up to 100%) mobile phases.
AQUA C18, Aquasil C18, Hypersil Gold AQ, YMC ODS-Aq
L1
Y
100
15
300
2.5 to 8.0
An excellent choice for the analysis of steroids, tetracyclines, drug metabolites, and other compounds that contain some degree of unsaturation.
A unique reversed-phase material that exhibits both increased retention and selectivity for aromatic and/or unsaturated compounds, compared to conventional alkyl and phenyl phases.
Unique
L11
Y
100
17
300
2.5 to 8.0
A very suitable choice for analysis of steroids, tetracyclines, drug metabolites, and other compounds that contain some degree of unsaturation.
A unique reversed-phase material that exhibits superior retention and selectivity for aromatic and/or unsaturated compounds, compared to conventional alkyl and phenyl phases. This column is a great alternative to our Biphenyl phase when increased retention is required.
Unique
L11
N
100
12
300
2.5 to 8.0
A polar group assists in deactivating surface silanols and contributes to unique separation selectivities for acids, bases, zwitterions, and other polar compounds.
One of a group of intrinsically base-deactivated (IBD) phases, with a polar group within, or intrinsic to, the alkyl bonded phase. Provides unique selectivity and high level of base deactivation while reducing or eliminating the need for mobile phase additives.
SymmetryShield, Discovery ABZ & ABZ+, Prism
L68
Y
100
11
300
2.5 to 8.0
Highly retentive for basic analytes. An excellent phase for separating nucleosides, nucleotides, purines, pyrimidines, and halogenated compounds.
A pentafluorophenyl phase with a propyl spacer.
Discovery HS F5, Luna PFP(2), Pursuit PFP
L43
Y
100
8
300
2.5 to 8.0
Excellent for basic pharmaceuticals, steroids (normal or reversed-phase conditions), or other basic compounds.
High-purity cyano phase with reduced silanol activity. Often a better choice than C18 for basic pharmaceuticals. Cyano is the most stable bonded phase for normal phase mode.
Platinum CN, Develosil Cyano, Luna CN, Hypersil Gold CN, Zorbax Eclipse XDB-CN
L10
N
100
2
300
2.5 to 8.0
Superior general-purpose amino phase. Ideal for carbohydrates.
Recommended for normal phase analyses of mono- and disaccharides and other similar compounds. Can also serve as a weak anion exchanger, with aqueous buffers.
Platinum Amino, Develosil NH2
L8
100
300
2.5 to 8.0
Ideal for normal phase applications.
High purity, high surface area.
L3

Specialty Columns

Restek Column
End Cap?
Pore Size (Å)
Carbon Load (%)
Surface Area (m2/g)
pH Range
Applications
Chromatographic Properties
Similar Phases
USP Code
90
Proprietary
130
2.0 to 8.0
Application-specific column for the analysis of ethanol metabolites ethyl glucuronide (EtG) and ethyl sulfate (EtS) in urine.
Reproducible retention of EtG and EtS resolved from matrix interferences.
Unique
60
450
2.5 to 8.0
Highly retentive phase for normal phase separation.
High purity, highly retentive phase for normal phase separation of polar analytes. Very high surface area. Recommended for MOSH/MOAH analysis.
Maxsil Si, LiChrospher Si-60
L3
Y
60
450
2.5 to 8.0
Ideal for the analysis of aldehydes and ketones as DNPH derivatives.
Highly retentive, highly selective phase, developed specifically for the analysis of aldehydes and ketones as DNPH derivatives.
Unique
N
60
450
2.5 to 8.0
Excellent resolution of challenging organic acids.
Single 30 cm column performs equally to two C18 columns in series. (AOAC Method 986.13)
Unique
N
110
180
2.5 to 8.0
Maximum resolution of polycyclic aromatic hydrocarbons.
Proprietary stationary phase; resolves 16 PAHs in US EPA Method 610. Silica manufactured by Restek.
Unique
100
300
2.5 to 8.0
Rapid analysis of carbamates.
Proprietary stationary phase can process up to twice as many samples per hour, compared to a conventional C18 phase.
Unique
100
300
2.5 to 8.0
Proprietary phase for the analysis of paraquat and diquat and other quaternary amines.
High-purity silica.
Unique

Large Molecule Columns

Restek Column
End Cap?
Pore Size (Å)
Carbon Load (%)
Surface Area (m2/g)
pH Range
Applications
Chromatographic Properties
Similar Phases
USP Code
Y
300
3.5
100
2.5 to 8.0
Proteins and other higher molecular weight compounds. Less retentive than C18 and C8 phases.
Silica manufactured by Restek with a narrow distribution around the 300Å mean pore size for more surface interaction and analyte retention.
BioBasic 4, Symmetry 300 C4, Jupiter 300 C4, Synchropak C4, 208 TP C4
L26
Y
300
5
100
2.5 to 8.0
Proteins and other higher molecular weight compounds. Less retentive than C18 phase.
Silica manufactured by Restek with a narrow distribution around the 300Å mean pore size for more surface interaction and analyte retention.
BioBasic 8, Zorbax 300 OSB C8, Synchropak C8, 208 TP C8
L7
Y
300
9
100
2.5 to 8.0
Proteins and other higher molecular weight compounds.
Silica manufactured by Restek with a narrow distribution around the 300Å mean pore size for more surface interaction and analyte retention.
BioBasic 18, Symmetry 300 C18, Jupiter 300 C18, Zorbax 300 OSB C18, Synchropak C18, 208 TP C18
L1
Y
300
7
100
2.5 to 8.0
Exhibits excellent peak shape for a wide range of compounds; ideal for large molecule and biomolecule assays.
Silica manufactured by Restek with a narrow distribution around the 300Å mean pore size for more surface interaction and analyte retention.
Unique
L11
Y
300
5
100
2.5 to 8.0
Exhibits excellent peak shape for a wide range of compounds, including nucleosides, nucleotides, and halogenated compounds.
Silica manufactured by Restek with a narrow distribution around the 300Å mean pore size for more surface interaction and analyte retention.
Unique
L43
300
100
2.5 to 8.0
Normal phase applications for highly retained high molecular weight compounds.
Silica manufactured by Restek with a narrow distribution around the 300Å mean pore size for more surface interaction and analyte retention.
L3