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Raptor™ SPP Core-Shell LC Columns

Raptor™ SPP Core-Shell LC Columns

SPP speed.

USLC® resolution

A new species of column.

  • Higher efficiency for drastically faster analysis times.
  • Better selectivity for substantially improved resolution.
  • Increased sample throughput with existing HPLC instrumentation.
  • Long-lasting ruggedness for dependable reproducibility.

The Dawn of an Era

Superficially porous particles (commonly referred to as SPP or “core-shell” particles) have been proven to provide fast separations without the need for expensive Ultra High Performance Liquid Chromatography™ (UHPLC) instruments, thereby increasing sample throughput without capital investment. These particles feature a solid, impermeable core enveloped by a thin, porous layer of silica that decreases the diffusion path and reduces peak dispersion. As a result, they offer significantly higher efficiency than traditional fully porous particles of similar dimensions—often rivaling the efficiency of smaller particles. Core-shell particles changed LC, but they were only the beginning....

A New Species Has Evolved

Restek is proud to announce that SPP core-shell technology has evolved with the introduction of Raptor™ LC columns. Although column efficiency, which is boosted with superficially porous particles, considerably accelerates analysis time, it has little effect on resolution (i.e., peak separation). Selectivity, on the other hand, has a substantial impact on resolution, but shows minimal improvement in analysis times. New Raptor™ LC columns bond rugged 2.7 and 5 μm superficially porous particles with Restek’s unique Ultra Selective Liquid Chromatography (USLC®) phases to offer chromatographers the best of both worlds.

By being the first to combine the speed of SPP with the resolution of highly selective USLC® technology, Raptor™ LC columns provide the practicing analyst with the most powerful tools available for fast and efficient method development. And because they are from Restek, Raptor™ LC columns are backed by the manufacturing and quality systems you’ve come to trust along with the best Plus 1 service in the industry. Choose them for all of your valued assays to experience Selectivity Accelerated.

Restek Literature Signup

Only Raptor™ LC columns
offer the higher efficiency of
a superficially porous particle
plus the improved resolution of
the USLC® phases.

Fully porous particles with typical stationary
phases show longer retention times and less resolution.

Ordinary superficially porous particles (SPP) with
typical stationary phases show shorter retention
times than fully porous particles, but still exhibit less resolution.

Only Raptor™ LC columns with highly selective
USLC® stationary phases show shorter
retention times and greater resolution.

Restek USLC Technology

Raptor FluoroPhenyl LC Columns (USP L43)

  • Capable of both reversed-phase and HILIC separations.
  • Ideal for increasing sensitivity and selectivity in LC-MS analyses.
  • Offers increased retention for charged bases.
  • Part of Restek’s Raptor LC column line featuring 2.7 and 5 μm SPP core-shell silica.
  • Switch to a Raptor FluoroPhenyl LC column when you need more retention and selectivity for basic and hydrophilic compounds than you can achieve on a C18.

Raptor C18 LC Columns (USP L1)

  • A traditional end-capped C18 ideal for general-purpose use in reversed-phase chromatography.
  • Wide pH range (2–8) provides excellent data quality for many applications, matrices, and compounds.
  • Offers the highest hydrophobic retention of any Raptor phase.
  • Part of Restek’s Raptor LC column line featuring 2.7 and 5 μm SPP core-shell silica.

Raptor ARC-18 LC Columns (USP L1)

  • Ideal for high-throughput LC-MS/MS applications with minimal sample preparation.
  • Well-balanced retention profile for better detection and integration of large, multiclass analyte lists.
  • Sterically protected to endure low-pH mobile phases without sacrificing retention or peak quality.
  • Part of Restek’s Raptor LC column line featuring 2.7 and 5 µm SPP core-shell silica.

Raptor Biphenyl LC Columns (USP L11)

  • Ideal for bioanalytical testing applications like drug and metabolite analyses.
  • Heightened selectivity and retention for compounds that are hard to resolve or elute early on C18 and other phenyl chemistries.
  • Limits ionization suppression and allows simple, MS-friendly mobile phases.
  • Restek’s most popular LC stationary phase applied to 2.7 and 5 µm Raptor SPP core-shell silica.

Raptor EXP Guard Column Cartridges

  • Free-Turn architecture lets you change cartridges by hand without breaking inlet/outlet fluid connectionsno tools needed.
  • Patented titanium hybrid ferrules can be installed repeatedly without compromising high-pressure seal.
  • Auto-adjusting design provides ZDV (zero dead volume) connection to any 10-32 female port.

EXP Direct Connect Holder

  • Free-Turn architecture lets you change cartridges by hand without breaking inlet/outlet fluid connectionsno tools needed.
  • Patented titanium hybrid ferrules can be installed repeatedly without compromising high-pressure seal.
  • Auto-adjusting design provides ZDV (zero dead volume) connection to any 10-32 female port.
  • EXP direct connect holder requires separate guard column cartridges; available from Restek in 2.1, 3.0, and 4.6 mm.

EXP Reusable Fittings for HPLC & UHPLC for 10-32 fittings and 1/16" tubing

EXP Hand-Tight Fittings

  • Hand-tight fitting style achieves effortless HPLC sealsno tools needed for a 8,700+ psi seal.
  • Both hand-tight and hex-head styles wrench-tighten for reliable UHPLC use up to 20,000+ psi!
  • Patented ferrule can be installed repeatedly without compromising high-pressure seal.
  • Hybrid design combines the durability of titanium with the sealing ability of PEEK.
  • Cutting-edge system provides ZDV (zero dead volume) connection to any 10-32 female port.
  • Compatible with 1/16" PEEK and stainless steel tubing.

Literature

Clinical, Forensic & Toxicology

“The Big Pain”: Development of Pain-Free Methods for Analyzing 231 Multiclass Drugs and Metabolites by LC-MS/MS

As the demand for testing of pain management drugs increases, many laboratories are turning to LC-MS/MS for its increased speed, sensitivity, and specificity. The methods shown here use a Raptor™ Biphenyl column because it provides fast, accurate analysis of 231 drugs and drug metabolites.

General Interest

The Effects of LC Particle Choice on Column Performance: Switching from 3 and 5 µm Fully Porous Particles (FPP) to 5 µm Superficially Porous Particles (SPP)

In this technical note, we will compare the performance of Raptor™ 5 µm superficially porous particle (SPP) LC columns to traditional 3 and 5 µm fully porous particle (FPP) LC columns. We will demonstrate how faster, more efficient analyses can be attained with greater sensitivity and reduced system pressure by switching from FPP to SPP—without altering method conditions.

General Interest

The Effects of LC Particle Choice on Column Performance: 2.7 vs. 5 µm Diameter Superficially Porous Particles (SPP)

Restek's Raptor™ LC columns feature superficially porous particles (commonly referred to as SPP or “core-shell” particles) and are available in both 2.7 and 5 µm particle sizes, giving analysts greater flexibility. However, it may not always be clear which particle size to choose. In this technical note, we will examine the differences in efficiency, sensitivity, and pressure between Raptor™ LC columns packed with 2.7 vs. 5 µm particles and provide advice on making the appropriate LC particle choice

Foods, Flavors & Fragrances

Featured Application: Multiclass Veterinary Antibiotics on Raptor™ C18 by LC-MS/MS - One Analysis, One Column, Less than 9 Minutes for Over 60 Multiclass Antibiotics

One Analysis, One Column, Less than 9 Minutes for Over 60 Multiclass Antibiotics: The use of antibiotics on food-producing animals is a public health and safety concern due to the potential of generating drug-resistant bacteria. A sensitive, efficient, and reliable analytical method for different classes of antibiotics is necessary to meet regulations covering maximum residue limits (MRL) for a variety of animal tissue and food products. The Raptor™ C18 LC column is the ideal choice.

Bioanalytical

Featured Application: 231 pain management and drugs of abuse compounds in under 10 minutes by LC-MS/MS - Big Pain Assays Aren't a Big Pain with the Raptor™ Biphenyl LC Column

The use of pain management drugs is steadily increasing. As a result, hospital and reference labs are seeing an increase in patient samples that must be screened for a wide variety of pain management drugs to prevent drug abuse and to ensure patient safety and adherence to their medication regimen. Restek's "Big Pain" LC-MS/MS methodology features a 231-compound multiclass drug and metabolite screen that can be performed in under 10 minutes with just one column: The Raptor™ Biphenyl.

Bioanalytical

Featured Application: LC Analysis of Vitamin D Epimers on Raptor FluoroPhenyl Columns - Fast, Accurate LC-MS/MS Analysis of the C3 Epimer of 25-Hydroxyvitamin D on Raptor FluoroPhenyl Columns

Vitamin D2 and D3 25-hydroxy metabolites are important diagnostic markers for vitamin D status, but these compounds cannot be distinguished from their epimeric forms on a C18 column. The Raptor FluoroPhenyl column provides ample chromatographic resolution for these isobaric epimers, which possess unequal bioactivities, so accurate results are generated and thus the proper diagnosis can be made.

Foods, Flavors & Fragrances

Featured Application: LC Analysis of 4-MEI on Raptor FluoroPhenyl Columns - Reliable HILIC LC-MS/MS Analysis of 4-Methylimidazole (4-MEI) on Raptor FluoroPhenyl Columns

Raptor FluoroPhenyl columns perform well in both HILIC and reversed phase modes, giving method developers the flexibility select the mode that performs best for their specific compounds of interest. Here we use a Raptor Fluorophenyl column in HILIC mode for LC analysis of 4-methylimidazole (4-MEI). This approach provides more retention than reversed phase mode and can be applied to determining 4-MEI levels in caramel colorings to monitor formulations and also in foods and beverages to assess potential exposure.

PDF Document

Raptor Biphenyl LC Columns Brochure

Raptor LC columns combine the speed of superficially porous particles (i.e., SPP or “core-shell”) with the resolution of highly selective USLC technology. Featuring Restek's most popular LC stationary phase, the rugged Raptor Biphenyl is extremely useful for fast separations in bioanalytical testing applications like drug and metabolite analyses, especially those that require a mass spectrometer (MS). (PDF - 6MB)

PDF Document

Dissecting Raptor LC Columns: A closer look at a new species

When we engineered our superficially porous particle (SPP or "core-shell") Raptor LC columns, we developed the bonding chemistries that are best suited to both the SPP construction and our highly selective USLC phases. But we didn't stop here. Take a closer look at a new species as we dissect the upgraded hardware and new, proprietary packing techniques behind Raptor LC columns and Raptor EXP guard columns. (PDF - 2MB)

PDF Document

Raptor ARC-18 LC Columns Brochure

Raptor LC columns combine the speed of superficially porous particles (i.e., SPP or “core-shell”) with the resolution of highly selective USLC technology. The sterically protected ARC-18 phase was designed and intended specifically for use with acidic mobile phases on LC-MS/MS systems. Even after extended use in low-pH (≤ 2.0) conditions, it offers consistent and well-balanced retention, peak shape, and response. Choose Raptor ARC-18 columns for the rapid analysis of large, multiclass assays by mass spectrometry. (PDF - 6MB)

PDF Document

Raptor™ SPP C18 Columns Sales Sheet

Every LC lab has a cache of C18s, but every C18 is not created equal. When you need a general-purpose LC column, don't just grab any C18. Choose the speed, efficiency, and long-lasting ruggedness of the new Raptor™ C18. (PDF - 2MB)

PDF Document

Featured Application: Multiclass Veterinary Antibiotics on Raptor™ C18 by LC-MS/MS

One Analysis, One Column, Less than 9 Minutes for Over 60 Multiclass Antibiotics: The use of antibiotics on food-producing animals is a public health and safety concern due to the potential of generating drug-resistant bacteria. A sensitive, efficient, and reliable analytical method for different classes of antibiotics is necessary to meet regulations covering maximum residue limits (MRL) for a variety of animal tissue and food products. The Raptor™ C18 LC column is the ideal choice. (PDF - 1MB)

PDF Document

Raptor™ C18 LC Columns Brochure

Raptor™ Speed, Efficiency, and Ruggedness—in C18: Every LC lab has a cache of C18s, but every C18 is not created equal. When you need a general-purpose LC column, don’t just grab any C18. Choose the speed, efficiency, and long-lasting ruggedness of the Raptor™ C18 (PDF - 2MB)

PDF Document

Raptor™ ARC-18 LC Columns Sales Sheet

Ahead of the Curve for Large, Multiclass Lists by Mass Spec: Designed and intended specifically for use on LC-MS/MS systems, the Raptor™ ARC-18 SPP LC column offers a well-balanced retention profile without the drawbacks of using an ordinary C18 in the harsh, acidic mobile phases needed for mass spectrometry (MS). (PDF - 2MB)

PDF Document

Raptor™ Biphenyl LC Columns Sales Sheet

Fast, Rugged Raptor™ Columns with Time-Tested Selectivity: The industry-leading Biphenyl, first introduced by Restek in 2005, is our most popular LC stationary phase because it is particularly adept at separating compounds that are hard to resolve or that elute early on C18 and other phenyl chemistries. As a result, the rugged Raptor™ Biphenyl column is extremely useful for fast separations in bioanalytical testing applications like drug and metabolite analyses, especially those that require a mass spectrometer (MS). (PDF - 4MB)

PDF Document

Raptor™ FluoroPhenyl LC Columns Sales Sheet

Restek's Raptor™ FluoroPhenyl phase gives you the flexibility to run in reversed-phase or HILIC modes so you can use the mode that is best for your target analytes. The Restek® Raptor™ FluoroPhenyl column is also amenable to LC-MS because it is extremely reliable and efficient with acidic mobile phases. (PDF - 2MB)

PDF Document

Raptor™ FluoroPhenyl LC Columns Brochure

Restek has now extended the speed and reliability of Raptor™ column technology into the HILIC realm with the addition of Raptor™ FluoroPhenyl columns. Restek's Raptor™ FluoroPhenyl phase offers chromatographers the ability to run in reversed-phase or HILIC mode for a variety of compounds. The Restek® Raptor™ FluoroPhenyl column is also amenable to LC-MS because it is extremely reliable and efficient with acidic mobile phases. (PDF - 3MB)

PDF Document

Infographic: Judging Raptor SPP Core-Shell Particles—2.7 vs. 5 µm Particle Sizes

Restek's Raptor™ superficially porous particle (SPP) LC columns are available in both 2.7 and 5 µm diameter particle sizes. This selection gives you greater flexibility to tailor your column to the task at hand—and our infographic takes the deliberation out of choosing the most appropriate size for your specific assay! (PDF - 3MB)