USLC™ Columns—Ultra Selective Liquid Chromatography™
Choose Columns Fast. Develop Methods Faster.
- Represents the widest range of reversed phase selectivity available, with just 4 stationary phases: Aqueous C18, IBD, Biphenyl, and PFP Propyl.
- Simplifies column choice for fast, effective method development.
- Offers greater control to more easily resolve closely eluting peaks.
- Maximizes column screening efficiency by ensuring orthogonality between analytical runs.
- Fits universally on any HPLC or UHPLC instrumentation.
Selectivity is the most influential factor affecting peak separation, or resolution. Restek Ultra Selective Liquid Chromatography™ (USLC™) technology is the directed application of orthogonal selectivity to help the practicing chromatographer develop methods more efficiently and more effectively. The Restek USLC™ column set offers the widest range of selectivity in the industry using just 4 unique stationary phases—Aqueous C18, IBD, Biphenyl, and PFP Propyl—to simplify phase selection.
Restek USLC™ columns are available on two silica lines to accommodate any instrument platform or chromatographic application. Pinnacle® DB columns (1.9 µm) feature base deactivated silica and are recommended for scalable HPLC and UHPLC analyses. Ultra columns (3 and 5 µm) use highest purity silica and are well suited for traditional HPLC analyses.
 USLC™ Method Development ToolboxAll the Right Tools—All in One Toolbox
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 Pinnacle® DB Biphenyl Columns (USP L11)particle size: 1.9µm, 3µm, or 5µm, spherical pore size: 140Å carbon load: 8% endcap: yes pH range: 2.5 to 8 temperature limit: 80°C Pinnacle® DB Biphenyl is a unique reversed phase material that displays both increased retention and selectivity for aromatic and/or unsaturated compounds when compared to conventional alkyl and phenyl phases. Highly base-deactivated spherical silica manufactured by Restek. An excellent choice for the analysis of steroids, tetracyclines, drug metabolites, and other compounds that contain some degree of unsaturation. Part of the Restek USLC™ column set, which offers the widest range of reversed phase selectivity available! |
 Ultra Biphenyl Columns (USP L11)particle size: 3µm or 5µm, spherical pore size: 100Å carbon load: 15% endcap: fully endcapped pH range: 2.5 to 8 temperature limit: 80°C A unique reversed phase material that exhibits both increased retention and selectivity for aromatic and/or unsaturated compounds, compared to conventional alkyl and phenyl phases. This is a great alternative to a C18 column when alternative selectivity is desired. An excellent choice for the analysis of steroids, tetracyclines, drug metabolites, and other compounds that contain some degree of unsaturation. Part of the Restek USLC™ column set, which offers the widest range of reversed phase selectivity available! |
Pinnacle® DB PFP Propyl Columns (USP L43)particle size: 1.9µm, 3µm, or 5µm, spherical pore size: 140Å carbon load: 6% endcap: yes pH range: 2.5 to 8 temperature limit: 80°C Pinnacle® DB PFP Propyl, a unique pentafluorophenyl phase with a propyl spacer, uses a highly base-deactivated spherical silica manufactured by Restek. This highly base-deactivated packing exhibits excellent peak shapes for a wide range of compounds, including nucleosides, nucleotides, and halogenated compounds. Part of the Restek USLC™ column set, which offers the widest range of reversed phase selectivity available! |
Ultra PFP Propyl Columns (USP L43)particle size: 3µm or 5µm, spherical pore size: 100Å carbon load: 15% endcap: fully endcapped pH range: 2.5 to 8 temperature limit: 80°C A pentafluorophenyl phase with a propyl spacer. Highly retentive for basic analytes. An excellent phase for separating nucleosides, nucleotides, purines, pyrimidines, and halogenated compounds. Part of the Restek USLC™ column set, which offers the widest range of reversed phase selectivity available! |
Pinnacle® DB Aqueous C18 Columns (USP L1)particle size: 1.9µm, 3µm, or 5µm, spherical pore size: 140Å carbon load: 6% pH range: 2.5 to 8 temperature limit: 80°C Highly selective phase for polar analytes. Compatible with highly aqueous (up to 100%) mobile phases. Silica manufactured by Restek. Part of the Restek USLC™ column set, which offers the widest range of reversed phase selectivity available! |
Ultra Aqueous C18 Columns (USP L1)particle size: 3µm or 5µm, spherical pore size: 100Å carbon load: 15% endcap: no pH range: 2.5 to 8 temperature limit: 80°C Highly retentive and selective for reversed phase separations of polar analytes. Highly base-deactivated. Compatible with highly aqueous (up to 100%) mobile phases. Part of the Restek USLC™ column set, which offers the widest range of reversed phase selectivity available! |
Pinnacle® DB IBD UHPLC Columns (USP L68)particle size: 1.9µm pore size: 140Å endcap: yes pH range: 2.5 to 8 temperature limit: 80°C An intrinsically base-deactivated (IBD) phase, containing a polar group within, or intrinsic to, the hydrocarbon bonded phase. Unique selectivity and a high level of base deactivation, while reducing or eliminating the need for mobile phase additives. Part of the Restek USLC™ column set, which offers the widest range of reversed phase selectivity available! |
Ultra IBD Columns (USP L68)Specialized Columns for Mixed Polar and Nonpolar Compoundsparticle size: 3µm or 5µm, spherical pore size: 100Å carbon load: 12% endcap: no pH range: 2.5 to 8 temperature limit: 80°C An intrinsically base-deactivated (IBD) phase, containing a polar group within, or intrinsic to, the hydrocarbon bonded phase. Unique selectivity and a high level of base deactivation, while reducing or eliminating the need for mobile phase additives. Part of the Restek USLC™ column set, which offers the widest range of reversed phase selectivity available! |
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 Column Choice: A Critical Factor for Successful UHPLC IntegrationColumn choice is a critical factor in successfully transferring methods between UHPLC and HPLC. Here, we discuss the column qualities that contribute to the successful integration of UHPLC technology. |
Novel Column Chemistry—High Impact, Low Cost TechnologyNovel column chemistries are a simple change in an already budgeted consumable that can lead to optimized and more reliable methods—giving a fast return on a minimal investment. |
Beyond C18—Increase Retention of Hydrophilic Compounds Using Biphenyl ColumnsThe Pinnacle® DB Biphenyl column offers enhanced retention and alternate selectivity for aromatic, unsaturated, and sulfur-containing hydrophilic compounds. Here we demonstrate significantly greater retention of sulfone- and sulfoxide-containing drug probes, compared to phenyl, phenyl hexyl, and alkyl (C18) columns. Only the Biphenyl column, using pi-pi interactions, separated both test probes to k′ > 2, the level needed to ensure separation from unretained matrix contaminants. |
 Comprehensive Pesticide Residue Analysis by LC/MS/MS Using an Ultra Aqueous C18 ColumnAnalysis of pesticide residues in foods is complicated by the large number of target compounds. LC/MS/MS analysis using an Aqueous C18 column provides more comprehensive results than GC alone. |
 Tech Tip — Why do I Lose Retention on my C18 in Highly Aqueous Mobile Phases?Learn what de-wetting is, and how to avoid it, by using an Aqueous C18 column, instead of a conventional C18, when analyzing polar compounds by HPLC. |
 USLC™ Columns: Choose Columns Fast. Develop Methods Faster.Restek USLC™ columns offer the widest range of selectivity available and are an integral part of successful method development. Ideal for column switching systems, these columns provide orthogonal separations to create optimal resolution and robust methods—all in a 4-column set. (PDF - 1813kB) |
 USLC™ Column Selection & Mobile Phase Adjustment GuideUSLC™ columns and this guide will help you easily choose the right stationary phase to target nearly any analyte in reversed phase or HILIC method development. It will also help you adjust your mobile phase to further improve results without guesswork or wasted time. (PDF - 1682kB) |
 USLC™ Method Development Toolbox: All the Right Tools—All in One BoxRestek’s USLC™ column set represents an incredible range of reversed phase selectivity with just 4 stationary phases: Aqueous C18, IBD, Biphenyl, and PFP Propyl. It simplifies column choice for fast, effective method development—and new USLC™ toolboxes make things even easier! (PDF - 681kB) |
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