Bioanalytical

Labs can save days of downtime by using an Rxi^®-5ms column in assays similar to this high-throughput test method for cholesterol and low-level sterol metabolites. Here, extremely reproducible results were obtained using an Rxi^®-5ms column, which gave highly consistent separations — even after 10,000 injections. Consistent performance and long column lifetimes increase productivity by reducing downtime associated with column changes and revalidation.

Viva Wide Pore HPLC columns are ideal for the separation of large molecules, as target analytes can enter the larger pores and access more of the surface area. Here we demonstrate the added retentive power of these columns, using the PEGylation of oxytocin as an example. Separation of these large, closely related compounds demonstrates the suitability of Viva Wide Pore columns for monitoring PEGylation reactions, and other large molecule applications.

A Viva C18 wide pore HPLC column resolves a tryptic digest of bovine serum albumin primarily into 1-2 peptide peaks, versus peaks of 3 or more peptides typically provided by conventional C18 columns. This superior resolution helps ensure more reliable identification of peptides in complex mixtures.