Clinical, Forensic & Toxicology

New Rtx®-BAC Plus columns outperform other blood alcohol column pairs and ensure baseline separation of all critical compounds. These columns provide definitive data in a fast, 2-minute analysis, so you can be certain of your results and maximize sample throughput. (PDF - 940kB)

New Rtx®-BAC Plus 1 and Rtx®-BAC Plus 2 columns provide definitive results quickly, so you can maximize sample throughput. These columns baseline separate all critical blood alcohol compounds, including ethanol, methanol, acetone, tert-butanol, acetaldehyde, isopropanol, and n-propanol, in less than 2 minutes. (PDF - 522kB)

Column selectivity has the most significant influence on chromatographic peak separation, or resolution, so choosing the right column can greatly speed up HPLC and UHPLC method development. In this article, we discuss column choice and identify a set of just 4 stationary phases—Restek’s USLC™ column set—that encompasses the widest range of reversed phase selectivity available today.

The aromatic retention of Ultra Biphenyl columns for pharmaceutical and drug-like compounds makes them ideal for pain panel analyses, such as the AB SCIEX Cliquid® pain method, as well as clinical methods for NSAIDs, THC and metabolites, synthetic cannabinoids, steroids, hormones, and sulfonamides in milk. (PDF - 566kB)

Sample throughput for benzodiazepines in urine and whole blood can be increased by adopting this dilute-and-shoot LC/MS/MS method which uses an Ultra Biphenyl HPLC column. Partial validation data are presented in this application note.

This application note details a fast extraction and analysis method for a wide range of metabolites of synthetic cannabinoids JWH-018 and JWH-073 in urine. Quantitative results are reported for carboxylated and mono-hydroxylated metabolites, including positional isomers.

Versatile Biphenyl columns provide excellent retention of both polar and nonpolar compounds, resulting in improved resolution of benzodiazepines, cannabinoids, and other key target compounds for clinical or forensic applications. (PDF - 633kB)

Rxi^®-5Sil MS columns produce excellent results for a number of forensic applications. The versatile selectivity separates a wide variety of compounds, which lets you keep analyzing samples instead of changing columns between methods. (PDF - 625kB)

Screening methods for consumer product adulteration cases, such as the recent Tylenol recall, can benefit from fast QuEChERS-based sample preparation and sensitive, full mass-range GC/TOF-MS.

An Rxi^®-5ms column will resolve acidic/neutral or free basic drugs under one set of conditions. There is no interference from column bleed — not even at 330°C. This is one of the first published applications for our new family of Rxi^® columns.

New Rxi^®-5Sil MS columns produce consistent results for amphetamine—even after 400 injections of derivatizing reagent—resulting in less time and money spent on column maintenance and replacement.

Conventional techniques for vitamin D analysis often lack adequate sensitivity, specificity, and speed. This LC/MS/MS assay results in highly symmetric peaks that elute in just 5 minutes.

The headspace (HS) analysis of gamma-hydroxybutyrate (GHB) described here reduces contamination and eliminates time-consuming derivatization. Confirmation testing using an Rtx^®-5MS column, provides definitive results in less than 7 minutes.

The headspace (HS) analysis of gamma-hydroxybutyrate (GHB) described here reduces contamination and eliminates time-consuming derivatization. Confirmation testing using an Rtx^®-5MS column, provides definitive results in less than 7 minutes. (PDF - 210kB)

Screening for evidence of marijuana use is typically done using an immunoassay method to detect derivatives in urine, but confirmation of positive results requires GC/MS. Here we describe a GC/MS method, using an Rxi®-5ms column, that resolves all major cannabinoid metabolites to baseline and exhibits very low bleed, even at 300°C. We also prolonged column life by baking at 340°C to remove derivatization by-products.

GC/MS analysis of urinary steroid hormones is a demanding application, and the Rxi®-1ms column meets the requirements for low bleed and inertness better than any column we have tested. We analyzed a variety of derivatized steroid sex hormones in less than 25 minutes, with excellent resolution and symmetric peaks. At 300°C or above, bleed from the Rxi^®-1ms column was negligible.

Basic drugs can interact with active sites on the surface of the inlet liner, reducing responses. The combination of a base-deactivated liner and a base-deactivated Rtx®-5Amine column ensures the greatest responses in analyses for these compounds.

Thermal desorption is used extensively for forensic science. This 16-page publication from Markes International Ltd. presents several key applications including drugs, arson accelerants, trace explosives, shotgun propellant, and inks. (PDF - 665kB)

Results of a blood alcohol analysis must be reliable. This 2-page publication describes carefully prepared, NIST-traceable, reference materials for this application, and lists GC columns that will complete the analysis in less than 3 minutes. (PDF - 80kB)

GC and LC methods can be used to "fingerprint" cocaine samples for law enforcement purposes. Here we show the analysis of simulated cocaine samples by both techniques and give column and instrument conditions for successful analysis. (PDF - 295kB)