Featured Application: Polycyclic Aromatic Hydrocarbons (PAHs) on a Pinnacle® II PAH HPLC Column

Fast Analysis of Polycyclic Aromatic Hydrocarbons (PAHs) by HPLC-UV


  • Quick ~15-minute analysis of PAHs by HPLC provides fast results without the expense of UHPLC equipment.
  • Baseline resolution improves accuracy for commonly analyzed PAHs of global concern.
  • Restek has method-specific PAH certified reference materials, as well as the only standard formulated to contain all major PAH interferences for priority PAHs.

Polycyclic aromatic hydrocarbons (PAHs) are a group of nonpolar organic molecules containing two or more benzene rings. PAHs are released from the incomplete combustion of organic fuels, and they generally occur as complex mixtures in our food and environment. Although not all polycyclic aromatic hydrocarbons are known to have detrimental health effects, several PAHs have been demonstrated to be toxic, carcinogenic, or mutagenic. To protect human health, many organizations have mandated exposure limits for PAHs. Air, water, soil, and food samples are commonly tested globally; however, the list of target compounds varies widely around the world. Many HPLC methods recommend a C18 column, but analysis times can be long (~30 minutes) and target analytes are not always completely resolved.

Restek’s Pinnacle® II PAH column was developed as an alternative to a C18 column specifically for the analysis of PAHs by HPLC. The stationary phase incorporates a proprietary bonding and unique selectivity that baseline resolves key PAH isomers, such as those in the European and U.S. PAH lists shown in the chromatograms below. These separations were accomplished in short analysis times (~15 minutes) on a conventional HPLC system (<400 bar pressure). Analyzing PAHs by HPLC with a Pinnacle® II PAH column allows labs to report PAH results quickly and accurately without investing in UHPLC equipment.


Peaks
1.Benzo[c]fluorene
2.Cyclopenta[cd]pyrene
3.Benzo[a]anthracene
4.Chrysene
5.5-Methylchrysene
6.Benzo(j)fluoranthene
7.Benzo[b]fluoranthene
8.Benzo[k]fluoranthene
Peaks
9.Benzo[a]pyrene
10.Dibenzo[a,l]pyrene
11.Dibenz[a,h]anthracene
12.Benzo[ghi]perylene
13.Indeno[1,2,3-cd]pyrene
14.Dibenzo[a,e]pyrene
15.Dibenzo(a,i)pyrene
16.Dibenzo(a,h)pyrene
EU 15+1 PAHs on Pinnacle® II PAH (UV/Vis)
LC_FF0529
ColumnPinnacle® II PAH (cat.# 921946E)
Dimensions:150 mm x 3.0 mm ID
Particle Size:4 µm
Pore Size:110 Å
Temp.:30 °C
SampleEU 15+1 PAH standard (cat.# 32470)
Diluent:Acetonitrile
Conc.:10 µg/mL (ppm)
Inj. Vol.:5 µL
Mobile Phase
A:Water
B:Acetonitrile:tetrahydrofuran (95:5)
Time (min)Flow (mL/min)%A%B
0.001.53070
3.001.52575
10.001.50100
14.501.50100
14.511.53070
16.51.53070
Max Pressure:275 bar
DetectorUV/Vis @ 292, 4 nm
Cell Temp:40 °C
Flow Cell Size:10 µL
InstrumentShimadzu Prominence

PeakstR (min)Conc.
(µg/mL)
1.Naphthalene2.68100
2.Acenaphthylene3.09100
3.1-Methylnaphthalene3.39100
4.2-Methylnaphthalene3.56100
5.Acenaphthene3.74100
6.Fluorene3.91100
7.Phenanthrene4.40100
8.Anthracene4.93100
9.Fluoranthene5.4910
PeakstR (min)Conc.
(µg/mL)
10.Pyrene5.9110
11.Benzo[a]anthracene7.3910
12.Chrysene7.7710
13.Benzo[b]fluoranthene8.9510
14.Benzo[k]fluoranthene9.525
15.Benzo[a]pyrene9.9910
16.Dibenzo[a,h]anthracene10.7510
17.Benzo[ghi]perylene11.1810
18.Indeno[1,2,3-cd]pyrene11.6010
EPA 8310 PAHs on Pinnacle® II PAH by LC-UV
LC_EV0537
ColumnPinnacle® II PAH (cat.# 921946E)
Dimensions:150 mm x 3.0 mm ID
Particle Size:4 µm
Pore Size:110 Å
Temp.:30 °C
SampleEPA Method 8310 quality control check (cat.# 31843)
Inj. Vol.:10 µL
Mobile Phase
A:Water
B:Acetonitrile
Time (min)Flow (mL/min)%A%B
0.001.25050
10.01.20100
12.51.20100
12.511.25050
15.01.25050
DetectorUV/Vis @ 280 nm

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