42th International Symposium on Capillary Chromatography and 15th GCxGC Symposium
Presented Thursday, May 17, 15:45–16:45 p.m.
Experience the Power of Pro EZGC Software and Accelerate Your Separations without a GC or Column
Presented by Jaap de Zeeuw and Chris Rattray
For more information, email Jaap de Zeeuw.
In this seminar, we will discuss the unique features of free Pro EZGC chromatogram modeling software, which allows you to optimize and model the GC separation of your specific analytes without using an actual GC. You can change carrier gas, column dimensions, gas velocity, and temperature programming—and then immediately see the results on your computer screen.
Using this software, we will also discuss practical examples of fast GC using new GC Accelerator oven insert kits. With a simple reduction of oven volume, it is possible to program the GC much faster, which allows shorter analysis times without investing in new instrumentation.
A New Low-Bleed, Inert, and Highly Efficient 65% Phenyl-Containing Polysiloxane Stationary Phase
Shawn Reese (presenter), Jaap de Zeeuw, Roy Lautamo, Jennifer Rutherford, Don Rhoads, Brian Salisbury, Colton Meyers, and Ashlee Reese
For more information, email Shawn Reese.
Capillary gas chromatography (GC) is a highly useful technique employed by chemists for difficult separation problems. Occasionally, there are applications that require a high-percentage phenyl-polysiloxane to afford the selectivity that a chemist desires. Historically, stationary phases that had >50% phenyl-containing siloxanes were difficult to coat in capillary dimensions. In addition, both the thermal stability and inertness of 65% phenyl siloxane polymers were limited and underperformed compared to their MS counterparts of lower polarity polysiloxane phases. Now, for the first time, a 65% polysiloxane polymer has been coated with much better efficiency than on previously columns and also is inert and low-bleed. This stationary phase has promise for many useful applications, including the analysis of mono-, di-, and triglycerides on a single column application.
RESTEK TECHNICAL POSTERS
C.20 Evaluation and Application of Large-Volume SPME Arrow Fibers
Jason S. Herrington, Colton Myers, Gary Stidsen, Steve Kozel, Jaap de Zeeuw, Christopher Rattray (presenter)
For more information, email Jason Herrington.
Solid phase microextraction (SPME) fibers were developed/patented in 1990 and licensed to Supelco until 2014. Consequently, SPME technology has remained virtually unchanged over the last 28 years and is subject to the following major drawbacks: limited mechanical stability and small phase volumes. A large volume fiber (SPME Arrow) has been developed to overcome these shortcomings. When compared to a traditional SPME fiber, the SPME Arrow’s increased diameter (1.1 mm vs. 0.5 mm) and length (20 mm vs. 10 mm) result in a larger sorption phase surface area (up to 6x) and volume (up to 20x), as well as a substantial increase in mechanical robustness. This presentation will provide a side-by-side comparison of SPME Arrows and traditional SPME fibers and an application of ISO 17943, which capitalizes on the advantages of the larger volume SPME design.
E.10 Gas Chromatographic Computer Modeling Software for Optimized Method Development
Chris English, Chris Nelson, Christopher Rattray, Jaap de Zeeuw (presenter), Kristi Sellers
For more information, email Jaap de Zeeuw.
Our recently introduced modeling software is a selectivity tool that relies on a pre-loaded library of thermodynamic retention indices. This makes it possible to predict retention times and optimize chromatographic methods without the need to analyze compound sets under many different conditions. The program allows the user to select the stationary phase and simultaneously adjust: film thickness, temperature, column length, column internal diameter, and flow. Users can enter each compound or cut/paste large lists of compounds into the program. Since its introduction there have been thousands of searches across a broad range of compound classes. The program outputs: compound retention time, resolution, and peak width, along with column conditions and dimensions. A model chromatogram is provided to illustrate retention, peak width, and resolution. Users have the option to view compound mass spectral data with the added benefit of overlaying mass spectra for coeluting analytes. Specific searches can be saved and accessed at a later date. Examples of these features will be presented with a focus on challenging separations.
E.11 Faster GC-MS Analysis with Short, Narrow-Bore Columns and Method Translation
Christopher Rattray (presenter), Matt Lininger, Jaap de Zeeuw, Mark Badger
For more information, email Chris Rattray.
Designed with GC-MS users in mind, the GC Accelerator kit provides a simple way to speed up sample analysis. By reducing oven volume, these inserts allow faster ramp rates to be attained, which reduces oven cycle time and allows for increased sample throughput and more capacity to process rush samples. When faster ramp rates are used, existing methods can be accurately scaled down to smaller, high-efficiency, narrow-bore columns using Restek’s EZGC method translator. With a scaled-down column, a properly translated method, and a GC Accelerator kit, you can obtain the same chromatographic separation—often with greater sensitivity—in a fraction of the time without making a capital investment.
L.06 New GC Inlet Liner Deactivation Exhibits Excellent Response for Active Compounds
Linx Waclaski, Mark Badger, Christopher Rattray, Jaap de Zeeuw (presenter)
For more information, email Linx Waclaski.
The choice of an inlet liner is critical to GC analysts, as it can have a large impact on the quality of chromatography and the data generated. Inertness is one of the most important factors to consider, because active analytes can easily be degraded or adsorbed within a hot GC inlet. A new liner deactivation was developed that has a high level of inertness towards sensitive compounds, a characteristic that is very consistent and reproducible from lot to lot. This liner deactivation was tested with a variety of pesticides, including organochlorine, organophosphorus, and organonitrogen classes. Other liner deactivations were also analyzed to compare performance for active compounds. All test liners were single taper with wool used in splitless mode, as this provides one of the most rugged assessments of deactivation quality.