Biphenyl Columns
 Ultra Biphenyl Columns (USP L11)particle size: 3µm or 5µm, spherical pore size: 100Å carbon load: 15% endcap: fully endcapped pH range: 2.5 to 8 temperature limit: 80°C A unique reversed phase material that exhibits both increased retention and selectivity for aromatic and/or unsaturated compounds, compared to conventional alkyl and phenyl phases. This is a great alternative to a C18 column when alternative selectivity is desired. An excellent choice for the analysis of steroids, tetracyclines, drug metabolites, and other compounds that contain some degree of unsaturation. Part of the Restek USLC™ column set, which offers the widest range of reversed phase selectivity available! |
 Pinnacle® DB Biphenyl Columns (USP L11)particle size: 1.9µm, 3µm, or 5µm, spherical pore size: 140Å carbon load: 8% endcap: yes pH range: 2.5 to 8 temperature limit: 80°C Pinnacle® DB Biphenyl is a unique reversed phase material that displays both increased retention and selectivity for aromatic and/or unsaturated compounds when compared to conventional alkyl and phenyl phases. Highly base-deactivated spherical silica manufactured by Restek. An excellent choice for the analysis of steroids, tetracyclines, drug metabolites, and other compounds that contain some degree of unsaturation. Part of the Restek USLC™ column set, which offers the widest range of reversed phase selectivity available! |
 Viva Biphenyl Columns (USP L11)particle size: 5µm pore size: 300Å carbon load: 6.7% endcap: yes pH range: 2.5 to 8 temperature limit: 80°C Highly base-deactivated wide pore packing that exhibits excellent peak shape for a wide range of compounds; ideal for large molecule and biomolecule assays. Highly retentive and selective phase for aromatic and unsaturated compounds, with increased retention, relative to phenyl phases. |
 Allure® Biphenyl Columns (USP L11)particle size: 5µm, spherical pore size: 60Å carbon load: 23% endcap: yes pH range: 2.5 to 8 temperature limit: 80°C Highly retentive and selective for aromatic and unsaturated compounds. Increased retention and selectivity, compared to phenyl phases. Excellent selectivity for steroids, tetracyclines, explosives, and other unsaturated compounds. |
 Pinnacle® II Biphenyl Columns (USP L11)particle size: 5µm, spherical pore size: 110Å endcap: yes pH range: 2.5 to 8 temperature limit: 80°C The Pinnacle® II Biphenyl phase offers alternate selectivity to straight-chain hydrocarbon phases, and enhanced selectivity and retention for unsaturated compounds, compared to traditional phenyl phases. An excellent confirmation column for explosive compounds, as in EPA method 8330. |
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 LC/MS/MS Analysis of Metabolites of Synthetic Cannabinoids JWH-018 and JWH-073 in UrineThis application note details a fast extraction and analysis method for a wide range of metabolites of synthetic cannabinoids JWH-018 and JWH-073 in urine. Quantitative results are reported for carboxylated and mono-hydroxylated metabolites, including positional isomers. |
 Easy Transfer of HPLC Methods to UHPLC Using Fully Scalable Pinnacle® DB ColumnsUltra High Pressure Liquid Chromatography (UHPLC) is a rapidly growing technique that can provide faster analysis times. Scaling conventional HPLC methods down to UHPLC can be an effective way to take advantage of shorter run times and increase sample throughput. Here we review the factors that must be considered when scaling down an existing method. A sulfonamides method transfer is used as an example; chromatograms and formulas for all required calculations are included. |
Optimize Selectivity & Efficiency in UHPLC Separations With More Stationary Phase Choices on 1.9µm Pinnacle™ DB HPLC ColumnsUltra-high pressure liquid chromatography (UHPLC) can significantly increase efficiency and produce faster separations. The small particle sizes used in UHPLC improve efficiency; however selectivity is still the most important factor affecting compound resolution. Here we demonstrate the importance of stationary phase choice in UHPLC separations. By optimizing selectivity for your analytes of interest, faster separations can be achieved without compromising resolution. |
Using π-π Interactions to Enhance Selectivity for Unsaturated Compounds The Allure™ Biphenyl HPLC ColumnRelative to phases that separate via hydrophobic or polar interactions, the Allure™ Biphenyl stationary phase offers better retention, selectivity, and efficiency, when analyzing compounds with differences in the numbers and locations of unsaturated bonds in the hydrocarbon ring structure. |
Beyond C18—Increase Retention of Hydrophilic Compounds Using Biphenyl ColumnsThe Pinnacle® DB Biphenyl column offers enhanced retention and alternate selectivity for aromatic, unsaturated, and sulfur-containing hydrophilic compounds. Here we demonstrate significantly greater retention of sulfone- and sulfoxide-containing drug probes, compared to phenyl, phenyl hexyl, and alkyl (C18) columns. Only the Biphenyl column, using pi-pi interactions, separated both test probes to k′ > 2, the level needed to ensure separation from unretained matrix contaminants. |
Separating NSAIDs through Aromatic Selectivity Improve Retention by Using An Allure® Biphenyl HPLC ColumnNon-steroidal anti-inflammatory drugs (NSAIDs) are typically separated on C18 phases. Separations on our Allure® Biphenyl HPLC column are based on pi-pi interactions, resulting in optimized retention and selectivity. Increased retention requires higher organic content in the mobile phase, increasing desolvation efficiency in LC/MS. Simple mobile phase changes enhance selectivity, making this column a great alternative to conventional phenyl phase columns, especially in method development. |
 Biphenyl: Leading Resolution in LC for Clinical and Forensic ApplicationsVersatile Biphenyl columns provide excellent retention of both polar and nonpolar compounds, resulting in improved resolution of benzodiazepines, cannabinoids, and other key target compounds for clinical or forensic applications. (PDF - 633kB) |
 Restek Ultra Biphenyl Columns: Next Generation Phenyl Columns are the Best Choice for Pain Panel AnalysesThe aromatic retention of Ultra Biphenyl columns for pharmaceutical and drug-like compounds makes them ideal for pain panel analyses, such as the AB SCIEX Cliquid® pain method, as well as clinical methods for NSAIDs, THC and metabolites, synthetic cannabinoids, steroids, hormones, and sulfonamides in milk. (PDF - 566kB) |
 Biphenyl: Next Generation of Phenyl ColumnsBiphenyl columns provide both high hydrophobic retention and aromatic selectivity in the same column. Orthogonal separations can be achieved with simple mobile phase changes, giving markedly better separations for a wide variety of molecules. (PDF - 531kB) |
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NSAIDs on Pinnacle® DB BiphenylLC_PH0475 |
Hormones on 1.9μm Pinnacle® DB BiphenylLC_PH0453 |
Synthetic Cannabinoids on Ultra BiphenylLC_CF0526 |
