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9169531
30mm
1.0mm
 
ea.
9169551
50mm
1.0mm
 
ea.
9169511
100mm
1.0mm
 
ea.
9169561
150mm
1.0mm
 
ea.
9169521
200mm
1.0mm
 
ea.
9169571
250mm
1.0mm
 
ea.
9169532
30mm
2.1mm
 
ea.
9169552
50mm
2.1mm
 
ea.
9169512
100mm
2.1mm
 
ea.
9169562
150mm
2.1mm
 
ea.
9169522
200mm
2.1mm
 
ea.
9169572
250mm
2.1mm
 
ea.
9169533
30mm
3.2mm
 
ea.
9169553
50mm
3.2mm
 
ea.
9169513
100mm
3.2mm
 
ea.
9169563
150mm
3.2mm
 
ea.
9169523
200mm
3.2mm
 
ea.
9169573
250mm
3.2mm
 
ea.
9169535
30mm
4.6mm
 
ea.
9169555
50mm
4.6mm
 
ea.
9169515
100mm
4.6mm
 
ea.
9169565
150mm
4.6mm
 
ea.
9169525
200mm
4.6mm
 
ea.
9169575
250mm
4.6mm
 
ea.
9169532-700
30mm
2.1mm
with Trident Inlet Fitting
ea.
9169552-700
50mm
2.1mm
with Trident Inlet Fitting
ea.
9169512-700
100mm
2.1mm
with Trident Inlet Fitting
ea.
9169562-700
150mm
2.1mm
with Trident Inlet Fitting
ea.
9169522-700
200mm
2.1mm
with Trident Inlet Fitting
ea.
9169572-700
250mm
2.1mm
with Trident Inlet Fitting
ea.
9169533-700
30mm
3.2mm
with Trident Inlet Fitting
ea.
9169553-700
50mm
3.2mm
with Trident Inlet Fitting
ea.
9169513-700
100mm
3.2mm
with Trident Inlet Fitting
ea.
9169563-700
150mm
3.2mm
with Trident Inlet Fitting
ea.
9169523-700
200mm
3.2mm
with Trident Inlet Fitting
ea.
9169573-700
250mm
3.2mm
with Trident Inlet Fitting
ea.
9169535-700
30mm
4.6mm
with Trident Inlet Fitting
ea.
9169555-700
50mm
4.6mm
with Trident Inlet Fitting
ea.
9169515-700
100mm
4.6mm
with Trident Inlet Fitting
ea.
9169565-700
150mm
4.6mm
with Trident Inlet Fitting
ea.
9169525-700
200mm
4.6mm
with Trident Inlet Fitting
ea.
9169575-700
250mm
4.6mm
with Trident Inlet Fitting
ea.

Boost signal-to-noise and sensitivity

For greatest signal-to-noise ratio and highest sensitivity in LC/MS methods, use maximum retentive HPLC phases, such as the Allure® family of packings. With these phases, stronger mobile phase solvents containing less aqueous content can be used. This increases the efficiency of gas phase analyte ion generation from the condensed phase, thereby increasing sensitivity.

Application-dedicated HPLC columns have longer lifetimes

Prolong the useful lifetimes of your HPLC columns by dedicating specific columns to specific applications, like analysts who use ion pair reagents often do. Sample components, mobile phase conditions, etc. can cause changes within a column, and sometimes effects of these changes are negligible until the column is used in another application. Although this practice can involve using more columns at one time, and calls for careful bookkeeping, longer lifetimes for individual columns can amount to significant savings.

Avoid the Void Volume

A speedy analysis is important, but avoid the temptation to elute important sample components near the void volume of your column. When you ignore this guideline, you are locked into using the mobile phase as the sample solvent; any admixing of sample solvent and mobile phase causes a baseline disturbance that makes quantification highly unreliable. Also, it is highly likely that the peak at the void volume will soar off-scale. You will avoid these and other potential problems by adjusting conditions such that the first target compound elutes with a capacity factor (k′) of at least 2.0.