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catalog #particle sizelengthidmodificationunits
9107331
3μm
30mm
1.0mm
 
ea.
9107351
3μm
50mm
1.0mm
 
ea.
9107311
3μm
100mm
1.0mm
 
ea.
9107361
3μm
150mm
1.0mm
 
ea.
9107332
3μm
30mm
2.1mm
 
ea.
9107352
3μm
50mm
2.1mm
 
ea.
9107312
3μm
100mm
2.1mm
 
ea.
9107362
3μm
150mm
2.1mm
 
ea.
9107333
3μm
30mm
3.2mm
 
ea.
9107353
3μm
50mm
3.2mm
 
ea.
9107313
3μm
100mm
3.2mm
 
ea.
9107363
3μm
150mm
3.2mm
 
ea.
9107335
3μm
30mm
4.6mm
 
ea.
9107355
3μm
50mm
4.6mm
 
ea.
9107315
3μm
100mm
4.6mm
 
ea.
9107365
3μm
150mm
4.6mm
 
ea.
9107332-700
3μm
30mm
2.1mm
with Trident Inlet Fitting
ea.
9107352-700
3μm
50mm
2.1mm
with Trident Inlet Fitting
ea.
9107312-700
3μm
100mm
2.1mm
with Trident Inlet Fitting
ea.
9107362-700
3μm
150mm
2.1mm
with Trident Inlet Fitting
ea.
9107333-700
3μm
30mm
3.2mm
with Trident Inlet Fitting
ea.
9107353-700
3μm
50mm
3.2mm
with Trident Inlet Fitting
ea.
9107313-700
3μm
100mm
3.2mm
with Trident Inlet Fitting
ea.
9107363-700
3μm
150mm
3.2mm
with Trident Inlet Fitting
ea.
9107335-700
3μm
30mm
4.6mm
with Trident Inlet Fitting
ea.
9107355-700
3μm
50mm
4.6mm
with Trident Inlet Fitting
ea.
9107315-700
3μm
100mm
4.6mm
with Trident Inlet Fitting
ea.
9107365-700
3μm
150mm
4.6mm
with Trident Inlet Fitting
ea.
9107531
5μm
30mm
1.0mm
 
ea.
9107551
5μm
50mm
1.0mm
 
ea.
9107511
5μm
100mm
1.0mm
 
ea.
9107561
5μm
150mm
1.0mm
 
ea.
9107521
5μm
200mm
1.0mm
 
ea.
9107571
5μm
250mm
1.0mm
 
ea.
9107532
5μm
30mm
2.1mm
 
ea.
9107552
5μm
50mm
2.1mm
 
ea.
9107512
5μm
100mm
2.1mm
 
ea.
9107562
5μm
150mm
2.1mm
 
ea.
9107522
5μm
200mm
2.1mm
 
ea.
9107572
5μm
250mm
2.1mm
 
ea.
9107533
5μm
30mm
3.2mm
 
ea.
9107553
5μm
50mm
3.2mm
 
ea.
9107513
5μm
100mm
3.2mm
 
ea.
9107563
5μm
150mm
3.2mm
 
ea.
9107523
5μm
200mm
3.2mm
 
ea.
9107573
5μm
250mm
3.2mm
 
ea.
9107535
5μm
30mm
4.6mm
 
ea.
9107555
5μm
50mm
4.6mm
 
ea.
9107515
5μm
100mm
4.6mm
 
ea.
9107565
5μm
150mm
4.6mm
 
ea.
9107525
5μm
200mm
4.6mm
 
ea.
9107575
5μm
250mm
4.6mm
 
ea.
9107532-700
5μm
30mm
2.1mm
with Trident Inlet Fitting
ea.
9107552-700
5μm
50mm
2.1mm
with Trident Inlet Fitting
ea.
9107512-700
5μm
100mm
2.1mm
with Trident Inlet Fitting
ea.
9107562-700
5μm
150mm
2.1mm
with Trident Inlet Fitting
ea.
9107522-700
5μm
200mm
2.1mm
with Trident Inlet Fitting
ea.
9107572-700
5μm
250mm
2.1mm
with Trident Inlet Fitting
ea.
9107533-700
5μm
30mm
3.2mm
with Trident Inlet Fitting
ea.
9107553-700
5μm
50mm
3.2mm
with Trident Inlet Fitting
ea.
9107513-700
5μm
100mm
3.2mm
with Trident Inlet Fitting
ea.
9107563-700
5μm
150mm
3.2mm
with Trident Inlet Fitting
ea.
9107523-700
5μm
200mm
3.2mm
with Trident Inlet Fitting
ea.
9107573-700
5μm
250mm
3.2mm
with Trident Inlet Fitting
ea.
9107535-700
5μm
30mm
4.6mm
with Trident Inlet Fitting
ea.
9107555-700
5μm
50mm
4.6mm
with Trident Inlet Fitting
ea.
9107515-700
5μm
100mm
4.6mm
with Trident Inlet Fitting
ea.
9107565-700
5μm
150mm
4.6mm
with Trident Inlet Fitting
ea.
9107525-700
5μm
200mm
4.6mm
with Trident Inlet Fitting
ea.
9107575-700
5μm
250mm
4.6mm
with Trident Inlet Fitting
ea.

Which MS Source is Best?

Question: Which MS source is better to use, atmospheric pressure chemical ionization (APCI) or electro-spray ionization (ESI)? Answer: In general, use APCI for small (<1000 Daltons), polar, thermally stable, neutral molecules, and/or where higher flow rates (> 1mL/min), higher buffer concentrations, or wider dynamic sample concentration ranges (3-4 orders of magnitude) are desired. Use ESI for a wide range of ionizable analytes (hydrophobic strong acids and bases work best) and/or where compounds are of high molecular weight, thermally labile, or non-volatile. APCI is mass sensitive, whereas, ESI appears to be concentration sensitive.

Application-dedicated HPLC columns have longer lifetimes

Prolong the useful lifetimes of your HPLC columns by dedicating specific columns to specific applications, like analysts who use ion pair reagents often do. Sample components, mobile phase conditions, etc. can cause changes within a column, and sometimes effects of these changes are negligible until the column is used in another application. Although this practice can involve using more columns at one time, and calls for careful bookkeeping, longer lifetimes for individual columns can amount to significant savings.

Avoid the Void Volume

A speedy analysis is important, but avoid the temptation to elute important sample components near the void volume of your column. When you ignore this guideline, you are locked into using the mobile phase as the sample solvent; any admixing of sample solvent and mobile phase causes a baseline disturbance that makes quantification highly unreliable. Also, it is highly likely that the peak at the void volume will soar off-scale. You will avoid these and other potential problems by adjusting conditions such that the first target compound elutes with a capacity factor (k′) of at least 2.0.