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catalog #particle sizelengthidmodificationunits
917835B
3μm
50mm
0.3mm
Capillary Column
ea.
917836B
3μm
150mm
0.3mm
Capillary Column
ea.
9178331
3μm
30mm
1.0mm
 
ea.
9178351
3μm
50mm
1.0mm
 
ea.
9178311
3μm
100mm
1.0mm
 
ea.
9178361
3μm
150mm
1.0mm
 
ea.
9178332
3μm
30mm
2.1mm
 
ea.
9178352
3μm
50mm
2.1mm
 
ea.
9178312
3μm
100mm
2.1mm
 
ea.
9178362
3μm
150mm
2.1mm
 
ea.
917833E
3μm
30mm
3.0mm
 
ea.
917835E
3μm
50mm
3.0mm
 
ea.
917831E
3μm
100mm
3.0mm
 
ea.
917836E
3μm
150mm
3.0mm
 
ea.
9178333
3μm
30mm
3.2mm
 
ea.
9178353
3μm
50mm
3.2mm
 
ea.
9178313
3μm
100mm
3.2mm
 
ea.
9178363
3μm
150mm
3.2mm
 
ea.
9178335
3μm
30mm
4.6mm
 
ea.
9178355
3μm
50mm
4.6mm
 
ea.
9178315
3μm
100mm
4.6mm
 
ea.
9178365
3μm
150mm
4.6mm
 
ea.
9178332-700
3μm
30mm
2.1mm
with Trident Inlet Fitting
ea.
9178352-700
3μm
50mm
2.1mm
with Trident Inlet Fitting
ea.
9178312-700
3μm
100mm
2.1mm
with Trident Inlet Fitting
ea.
9178362-700
3μm
150mm
2.1mm
with Trident Inlet Fitting
ea.
9178333-700
3μm
30mm
3.2mm
with Trident Inlet Fitting
ea.
9178353-700
3μm
50mm
3.2mm
with Trident Inlet Fitting
ea.
9178313-700
3μm
100mm
3.2mm
with Trident Inlet Fitting
ea.
9178363-700
3μm
150mm
3.2mm
with Trident Inlet Fitting
ea.
9178335-700
3μm
30mm
4.6mm
with Trident Inlet Fitting
ea.
9178355-700
3μm
50mm
4.6mm
with Trident Inlet Fitting
ea.
9178315-700
3μm
100mm
4.6mm
with Trident Inlet Fitting
ea.
9178365-700
3μm
150mm
4.6mm
with Trident Inlet Fitting
ea.
9178531
5μm
30mm
1.0mm
 
ea.
9178551
5μm
50mm
1.0mm
 
ea.
9178511
5μm
100mm
1.0mm
 
ea.
9178561
5μm
150mm
1.0mm
 
ea.
9178521
5μm
200mm
1.0mm
 
ea.
9178571
5μm
250mm
1.0mm
 
ea.
9178532
5μm
30mm
2.1mm
 
ea.
9178552
5μm
50mm
2.1mm
 
ea.
9178512
5μm
100mm
2.1mm
 
ea.
9178562
5μm
150mm
2.1mm
 
ea.
9178522
5μm
200mm
2.1mm
 
ea.
9178572
5μm
250mm
2.1mm
 
ea.
9178533
5μm
30mm
3.2mm
 
ea.
9178553
5μm
50mm
3.2mm
 
ea.
9178513
5μm
100mm
3.2mm
 
ea.
9178563
5μm
150mm
3.2mm
 
ea.
9178523
5μm
200mm
3.2mm
 
ea.
9178573
5μm
250mm
3.2mm
 
ea.
9178535
5μm
30mm
4.6mm
 
ea.
9178555
5μm
50mm
4.6mm
 
ea.
9178515
5μm
100mm
4.6mm
 
ea.
9178565
5μm
150mm
4.6mm
 
ea.
9178525
5μm
200mm
4.6mm
 
ea.
9178575
5μm
250mm
4.6mm
 
ea.
9178532-700
5μm
30mm
2.1mm
with Trident Inlet Fitting
ea.
9178552-700
5μm
50mm
2.1mm
with Trident Inlet Fitting
ea.
9178512-700
5μm
100mm
2.1mm
with Trident Inlet Fitting
ea.
9178562-700
5μm
150mm
2.1mm
with Trident Inlet Fitting
ea.
9178522-700
5μm
200mm
2.1mm
with Trident Inlet Fitting
ea.
9178572-700
5μm
250mm
2.1mm
with Trident Inlet Fitting
ea.
9178533-700
5μm
30mm
3.2mm
with Trident Inlet Fitting
ea.
9178553-700
5μm
50mm
3.2mm
with Trident Inlet Fitting
ea.
9178513-700
5μm
100mm
3.2mm
with Trident Inlet Fitting
ea.
9178563-700
5μm
150mm
3.2mm
with Trident Inlet Fitting
ea.
9178523-700
5μm
200mm
3.2mm
with Trident Inlet Fitting
ea.
9178573-700
5μm
250mm
3.2mm
with Trident Inlet Fitting
ea.
9178535-700
5μm
30mm
4.6mm
with Trident Inlet Fitting
ea.
9178555-700
5μm
50mm
4.6mm
with Trident Inlet Fitting
ea.
9178515-700
5μm
100mm
4.6mm
with Trident Inlet Fitting
ea.
9178565-700
5μm
150mm
4.6mm
with Trident Inlet Fitting
ea.
9178525-700
5μm
200mm
4.6mm
with Trident Inlet Fitting
ea.
9178575-700
5μm
250mm
4.6mm
with Trident Inlet Fitting
ea.

Avoiding Chain Collapse

Moderate to very weak, polar solutes may require high aqueous mobile phases in order to achieve retention on C18 phases. Unfortunately, most C18 packings experience chain collapse under these conditions. This is chromatographically manifested as a partial or complete loss of retention, with analytes eluting at or close to the solvent front. To avoid this, choose the collapse-immune Ultra Aqueous C18 phase, or alternative packings that are resistant to chain folding, such as Allure® Basix or Ultra IBD.

Application-dedicated HPLC columns have longer lifetimes

Prolong the useful lifetimes of your HPLC columns by dedicating specific columns to specific applications, like analysts who use ion pair reagents often do. Sample components, mobile phase conditions, etc. can cause changes within a column, and sometimes effects of these changes are negligible until the column is used in another application. Although this practice can involve using more columns at one time, and calls for careful bookkeeping, longer lifetimes for individual columns can amount to significant savings.

Avoid the Void Volume

A speedy analysis is important, but avoid the temptation to elute important sample components near the void volume of your column. When you ignore this guideline, you are locked into using the mobile phase as the sample solvent; any admixing of sample solvent and mobile phase causes a baseline disturbance that makes quantification highly unreliable. Also, it is highly likely that the peak at the void volume will soar off-scale. You will avoid these and other potential problems by adjusting conditions such that the first target compound elutes with a capacity factor (k′) of at least 2.0.