GC Potency Analysis for Marijuana on Rxi®-5Sil MS
| Peaks | RT (min) | Conc. (wt.%) | |
|---|---|---|---|
| 1. | CBD | 4.035 | 0.0 |
| 2. | THC | 4.524 | 3.6 |
| 3. | CBN | 4.840 | 0.3 |
GC_GN1156
| Column | Rxi®-5Sil MS, 15 m, 0.25 mm ID, 0.25 µm (cat.# 13620) |
| Injection | |
| Inj. Vol.: | 1 µL split (split ratio 20:1) |
| Liner: | Sky™ 4.0 mm ID single taper/gooseneck inlet liner w/wool (cat.# 23303.5) |
| Inj. Temp.: | 250 °C |
| Oven | |
| Oven Temp: | 200 °C (hold 0 min) to 300 °C at 15 °C/min (hold 0 min) |
| Carrier Gas | H2, constant pressure (7 psi, 48.3 kPa) |
| Temp.: | 200 °C |
| Dead Time: | 0.6 min @ 200 °C |
| Detector | FID @ 300 °C |
| Make-up Gas Flow Rate: | 45 mL/min |
| Make-up Gas Type: | N2 |
| Instrument | HP5890 GC |
| Notes | Blue trace = cannabinoids standard (cat.# 34014) diluted to 100 µg/mL in isopropyl alcohol. Red trace = extracted marijuana sample Sample extraction: Weigh 0.2 g of sample into a 40 mL VOA vial, add 40 mL of isopropyl alcohol, shake for 5 minutes, and allow sample to settle. Quantification: Potency values (weight%) were based on a 1-point standard curve using the standard show above. |
