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Nicotine-Related Compounds in Urine on Raptor™ Biphenyl by LC-MS/MS

Nicotine-Related Compounds in Urine on Raptor™ Biphenyl by LC-MS/MS

PeakstR (min)Precursor IonProduct Ion
1.Nornicotine1.25149.1080.05
2.Norcotinine1.81163.0980.05
3.trans-3'-Hydroxycotinine1.88193.1280.05
4.Nicotine1.95163.15132.10
5.Anabasine2.20163.1391.63
6.Cotinine2.88177.1298.07
image
LC_CF0606
ColumnRaptor™ Biphenyl (cat.# 9309512)
Dimensions:100 mm x 2.1 mm ID
Particle Size:5 µm
Pore Size:90 Å
Guard Column:Raptor™ Biphenyl EXP® cartridges 5 mm, 2.1 mm ID, 5 µm (cat.# 930950252)
Temp.:30 °C
Sample
Diluent:Water
Conc.:25 ng/mL (fortified human urine)
Inj. Vol.:5 µL
Mobile Phase
A:0.1% Formic acid, 5 mM ammonium formate in water
B:0.1% Formic acid in methanol
Time (min)Flow (mL/min)%A%B
0.000.49010
1.000.49010
2.000.47030
3.000.43070
3.010.49010
5.000.49010
DetectorMS/MS
Ion Mode:ESI+
Mode:MRM
InstrumentUHPLC
NotesThe urine from a non-tobacco user was fortified with analytes to a final concentration of 25 ng/mL and prepared by a liquid-liquid extraction method. A 250 μL urine sample was mixed with 50 μL of 5N NaOH and extracted with 1.5 mL of 50/50 methylene chloride/diethyl ether. A portion of organic phase (1.0 mL) was dried and reconstituted with 200 μL of water for analysis.
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Nicotine

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biphenyl

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raptor

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