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Multiclass Drug Panel on Raptor™ Biphenyl by LC-MS/MS

Multiclass Drug Panel on Raptor™ Biphenyl by LC-MS/MS

PeakstR (min)Conc.
(ng/mL)
Precursor IonProduct IonProduct Ion
1.trans-3-Hydroxycotinine1.3225193.180.0134.0
2.Norcotinine1.3325163.180.1118.1
3.Nicotine1.4025163.2130.1117.1
4.BZP1.4725177.291.165.1
5.Anabasine1.5325163.2120.180.1
6.Noroxymorphone1.7825288.1270.1212.9
7.Lisdexamfetamine1.7925264.284.191.0
8.Phenelzine sulfate1.9025137.2105.177.1
9.Acetaminophen1.93100152.293.065.0
10.Pregabalin2.2125160.2142.155.0
11.Cotinine2.2725177.280.098.1
12.Morphine2.2725286.2152.1165.0
13.(+)-Ephedrine2.3725166.2148.1115.0
14.Oxymorphone2.3825302.1227.2198.2
15.Hydromorphone2.6125286.2184.9156.9
16.Amphetamine2.6325136.291.065.1
17.DMAA2.6525116.157.199.2
18.Gabapentin2.7125172.2154.0137.1
19.Methylephedrine2.8325180.2162.0115.1
20.Levetiracetam2.8425171.1125.969.2
21.Desmethylolanzapine2.9425299.1256.1198.0
22.Norcodeine2.9625286.1151.9115.0
23.Olanzapine3.1225313.2256.1198.1
24.Methamphetamine3.1325150.391.1119.0
25.MDA3.1825180.1163.1105.2
26.(-)-Naloxone3.2725328.3310.1212.3
27.Phentermine3.2925150.291.1133.1
28.Noroxycodone3.3225302.1227.0197.9
29.Methylone3.3625208.1160.1132.1
30.O-Desmethyl-cis-tramadol3.3725250.158.042.0
31.(-)-Dihydrocodeine3.3825302.2199.0128.2
32.Norhydrocodone3.4325286.1199.0128.2
33.Codeine3.4325300.2152.0165.1
34.6-Acetylmorphine3.4725328.2165.0211.0
35.MDMA3.6025194.2163.0135.1
36.Desomorphine3.6125272.1152.1165.0
37.Oxycodone3.6425316.2298.0169.0
38.Naltrexone3.7125342.2324.0267.0
39.Levamisole3.7525205.1178.091.1
40.Hydrocodone3.7625300.1199.0128.0
41.Diethylpropion3.8025206.2105.1100.1
42.Mephedrone3.8025178.2145.0160.1
43.2-Oxo-3-hydroxy-LSD3.8225356.3237.0222.0
44.o-Desmethylvenlafaxine3.8425264.158.1107.0
45.6-β-Naltrexol3.9125344.3326.1308.1
46.Tapentadol-β-D-glucuronide3.9125398.3222.1107.0
47.7-Aminonitrazepam3.9525252.2121.194.2
48.MDEA4.0025208.1163.0105.2
49.m-Hydroxybenzoylecgonine4.0225306.1168.1121.0
50.Lamotrigine4.0925256.0211.1145.0
51.Ritalinic acid4.1125220.184.156.2
52.N-Desmethyltapentadol4.1625208.1121.2107.1
53.Norbuprenorphine glucuronide4.1825590.5414.3165.0
54.Zonisamide4.2325212.9132.177.1
55.mCPP4.2625197.1154.0118.1
56.Norketamine4.2825224.1125.089.1
57.Nalbuphine4.3325358.2340.1115.0
58.Norfentanyl4.3325233.184.155.0
59.(+/-)-Hydroxybupropion4.3525256.0130.2166.0
60.Tapentadol4.3725222.1107.1121.0
61.Lacosamide4.4725251.591.1108.1
62.Dextrorphan4.5025258.2157.0201.2
63.Levorphanol4.5025258.2156.8199.2
64.7-Hydroxyquetiapine4.5125400.3269.0208.0
65.Molindone4.5125277.2100.170.2
66.Tramadol4.5225264.258.077.1
67.Zolpidem phenyl-4-carboxylic acid4.6025338.2265.1219.0
68.Felbamate4.6225239.1117.1177.8
69.N-Desmethyl-cis-tramadol4.6525250.244.0232.1
70.Ketamine4.6625238.1125.189.1
71.Benzoylecgonine4.6725290.1168.177.1
72.Methylphenidate4.6825234.184.156.1
73.Normeperidine4.7625234.1160.291.0
74.Meprobamate4.7925219.1158.297.0
75.Meperidine4.8025248.1220.1174.1
76.Bupropion4.8125240.0184.1130.2
77.Heroin4.8525370.4328.2164.9
78.Cocaine5.0125304.2182.077.1
79.7-Aminoclonazepam5.0225286.1121.2250.1
80.Zopiclone5.0425389.2245.0217.2
81.N-Desmethylmirtazapine5.1025252.1195.1209.2
82.Norcocaine5.1125290.1168.0136.1
83.Phenobarbital*5.11500230.9187.885.0
84.Buprenorphine glucuronide5.1225644.3468.255.1
85.MDPV5.1525276.2135.1126.1
86.Venlafaxine5.1525278.4260.4121.2
87.Milnacipran5.2425247.2100.1129.1
88.Pentazocine5.2925286.2218.169.2
89.Mirtazapine5.3125266.1195.172.1
90.Butalbital*5.31500223.0180.084.9
91.Norbuprenorphine5.3550414.3152.2165.2
92.LSD5.3925324.3223.1208.0
93.Butorphanol5.4325328.3310.3131.2
94.Fosphenytoin5.4325363.1265.9207.8
95.Topiramate5.4425340.2264.2223.9
96.Cocaethylene5.4825318.2196.182.0
97.Clozapine5.4825328.2271.1193.1
98.9-Hydroxyrisperidone5.5125427.3207.1110.2
99.Acetyl fentanyl5.5825323.3188.0105.0
100.Didesmethyl citalopram HCl5.7325297.1261.9109.1
101.7-Aminoflunitrazepam5.7525284.1135.0227.1
102.Amobarbital*5.75500225.0182.084.8
103.Zolpidem5.7625308.2235.2218.9
104.N-Desmethylcitalopram5.7725311.1262.1109.1
105.Isocarboxazid5.7725232.091.165.2
106.Pentobarbital*5.79500225.0182.084.9
107.Escitalopram5.8025325.3109.1261.9
108.Citalopram5.8025325.2109.1262.0
109.Diphenhydramine5.8025256.1167.0152.1
110.Desmethyldoxepin5.8825266.1107.1115.0
111.Ezogabine5.9125304.0230.1109.1
112.Doxepin5.9625280.1107.277.1
113.Haloperidol5.9625377.2123.095.1
114.Fluvoxamine5.9725319.371.1257.8
115.Trazodone5.9925372.3176.1148.0
116.Norfluoxetine6.0125296.3134.3104.9
117.Fentanyl6.0225337.3188.0105.1
118.Oxcarbazepine6.0225253.1180.0208.1
119.Risperidone6.0325411.2191.0110.1
120.Buprenorphine6.0450468.355.1414.2
121.Fluoxetine6.0525310.1148.0115.1
122.Secobarbital*6.08500237.0193.984.8
123.Phenytoin6.1225253.0103.9181.7
124.Carisoprodol6.1325261.1176.062.0
125.Flurazepam6.1425388.2315.2183.0
126.Chlordiazepoxide6.1425300.1282.0227.0
127.Dextromethorphan6.1625272.2215.1170.9
128.PCP6.1825244.286.1159.1
129.Tenoxicam6.1825338.1121.095.0
130.Buspirone6.2025386.3122.195.0
131.Quetiapine6.2825384.2253.0221.2
132.Norpropoxyphene6.2925326.4252.091.1
133.Propoxyphene6.3025340.3265.958.1
134.Asenapine6.3525286.2165.1229.1
135.Ziprasidone6.4025413.2194.1130.0
136.Protriptyline6.4225264.1191.1165.2
137.Promazine6.4225285.158.086.0
138.Midazolam6.4325326.2291.1248.9
139.Desipramine6.4325267.172.1193.1
140.Amoxapine6.4425314.2271.0193.1
141.Sufentanil6.4825387.2238.1111.1
142.Imipramine6.5125281.186.258.1
143.Paroxetine6.5125330.2192.270.1
144.Loxapine6.5225328.1271.1193.0
145.Maprotiline6.5225278.1250.2191.0
146.Duloxetine6.5225298.1154.1188.2
147.Metaxalone6.5225222.1161.177.1
148.Carbamazepine6.5510237.1194.1165.0
149.Cyclobenzaprine6.5625276.2215.0189.0
150.EDDP6.5725278.2234.3249.2
151.Nortriptyline6.5825264.191.1115.2
152.Mitragynine6.5925399.3174.2159.0
153.Eslicarbazepine6.6025297.2194.0237.1
154.Amitriptyline6.6525278.191.1202.1
155.α-Hydroxymidazolam6.7025342.2324.1202.9
156.Trimipramine6.7525295.2100.258.2
157.N-Desmethylflunitrazepam6.8025300.1254.1198.1
158.Lorazepam6.8225321.1275.0229.0
159.Mesoridazine6.8325387.2126.198.2
160.Tiagabine6.8925376.2246.8111.1
161.Ketorolac6.9025256.1104.977.0
162.Chlorpromazine6.9125320.158.186.1
163.Loxoprofen*6.93200245.083.280.9
164.Clomipramine6.9525315.386.058.0
165.Oxazepam6.9625287.1241.2268.8
166.Methadone6.9825310.2264.9105.1
167.Sertraline6.9825306.2158.9275.1
168.Dehydro aripiprazole7.0125446.2285.098.1
169.Clonazepam7.0125316.1270.0214.1
170.Nitrazepam7.0125282.1235.9180.1
171.Lornoxicam7.02100373.1121.195.0
172.25I-NBOMe7.0525428.1121.291.1
173.Salsalate*7.05100256.8136.892.9
174.Desalkylflurazepam7.0925289.0140.1104.0
175.Tolmetin7.1025258.1119.191.0
176.α-Hydroxytriazolam7.1225359.1330.9175.9
177.Fluphenazine7.1625438.3171.1143.2
178.2-Hydroxyethylflurazepam7.1625333.1211.1109.0
179.Ketoprofen*7.17100253.0209.0-
180.Naproxen*7.17200229.0168.9184.9
181.EMDP7.1925264.2235.2220.3
182.Aripiprazole7.2025448.2285.0176.1
183.Perphenazine7.2225404.2171.1143.2
184.Etoricoxib7.2425359.2280.0279.2
185.Methaqualone7.2425251.191.1132.2
186.α-Hydroxyalprazolam7.2525325.1297.0216.2
187.Trifluoperazine7.2825408.2141.2113.1
188.Piroxicam7.3425332.195.278.1
189.Ibuprofen*7.34200205.0160.9-
190.Prochlorperazine7.3625374.1141.0113.1
191.Etodolac*7.3650286.0241.9212.0
192.Nordiazepam7.3725271.0139.9208.0
193.Phenazepam7.3925349.1206.2179.0
194.Fenoprofen*7.40200240.992.8-
195.Zaleplon7.4325306.1264.0236.0
196.Clobazam7.4325301.0259.1224.0
197.Flurbiprofen*7.48200243.0199.0196.8
198.Thiothixene7.5025444.2221.2235.0
199.Flunitrazepam7.5525314.2267.9239.1
200.Meloxicam7.5525352.1115.0141.0
201.Triazolam7.5825343.1307.9315.0
202.Estazolam7.5825295.1266.9205.1
203.Celecoxib7.6025382.2362.0300.1
204.Parecoxib7.6025371.2234.0165.1
205.Temazepam7.6125301.1255.1282.9
206.Sulindac7.7025357.2233.1248.0
207.Alprazolam7.7225309.1280.9204.9
208.Flufenamic acid*7.7550279.9236.1176.2
209.Thioridazine7.7625371.2126.198.1
210.Diclofenac*7.78100293.9249.8-
211.Nabumetone7.8025229.1171.1128.1
212.Oxaprozin7.8725294.1103.0234.2
213.HU-2118.0325387.3155.9128.1
214.JWH‐2008.0425385.3155.0127.1
215.Diazepam8.0425285.1192.9153.9
216.Mefenamic acid8.0425242.1224.1-
217.Tolfenamic acid*8.1750259.9215.8-
218.Meclofenamic acid*8.18200293.9257.8177.7
219.(+/-)-CP 47,497*8.27200317.1245.1159.0
220.Indomethacin*8.2950356.0311.8296.9
221.Perampanel8.3225350.2219.1247.1
222.(+/-)-CP 47,497 C8 homologue*8.42200331.1259.0159.1
223.11-Nor-9-carboxy-delta-9-THC*8.4425343.0298.9244.8
224.Prazepam8.5125325.2271.0139.9
225.XLR-118.6025330.3125.2144.0
226.Δ9-THC8.7625315.2193.0123.2
227.UR-1448.7825312.3125.1214.1
228.JWH-2508.9625336.3120.991.1
229.THCA-A*9.1225357.1312.9245.0
230.JWH-0739.2125328.2155.0127.1
231.JWH‐0189.3525342.3127.1155.1
*Analyzed using negative polarity.
image
LC_CF0621
ColumnRaptor™ Biphenyl (cat.# 9309A12)
Dimensions:100 mm x 2.1 mm ID
Particle Size:2.7 µm
Guard Column:Raptor™ Biphenyl EXP® guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252)
Temp.:30 °C
Sample
Diluent:Water
Inj. Vol.:5 µL
Mobile Phase
A:Water + 0.1% formic acid + 2 mM ammonium formate
B: Methanol + 0.1% formic acid + 2 mM ammonium formate
Time (min)Flow (mL/min)%A%B
0.000.6955
9.000.60100
10.000.60100
10.010.6955
12.000.6955
DetectorMS/MS
Ion Mode:ESI+/ESI-
Mode:Scheduled MRM
InstrumentUHPLC
Notes1. Positive and negative polarity data were collected simultaneously from a single injection.
2. Ibuprofen, fosphenytoin, and topiramate degrade quickly in solution.
3. Levorphanol and dextrorphan are enantiomers and are not resolved.
4. Escitalopram is a pure enantiomer and citalopram is a racemic mixture of both enantiomers. These compounds are not resolved.
5. DMAA consists of 4 stereoisomers, 2 of which are partially resolved.
6. Doxepin and desmethylydoxepin consist of a mixture of cis/trans isomers. The isomers are fully resolved and the retention time identifies the main isomer component.
7. Lornoxicam, meloxicam, etodolac, oxaprozin, indomethacin, and mefenamic acid can be detected by positive or negative polarity. The polarity chosen in this chromatogram provides optimal response.
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