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Fentanyl and Analogues in Urine on Raptor Biphenyl by LC-MS/MS
|Column||Raptor Biphenyl (cat.# 9309552)|
|Dimensions:||50 mm x 2.1 mm ID|
|Particle Size:||5 µm|
|Pore Size:||90 Å|
|Guard Column:||Raptor Biphenyl EXP guard cartridge 5 mm, 2.1 mm ID, 5 µm (cat.# 930950252)|
|Inj. Vol.:||5 µL|
|A:||0.1% Formic acid in water|
|B:||0.1% Formic acid in methanol|
|Notes||Human urine was fortified at 10 ng/mL with target analytes. An 80 μL urine aliquot was mixed with 320 μL of 70:30 water:methanol solution (5-fold dilution) and 10 μL of internal standard solution (40 ng/mL in methanol) in a Thomson SINGLE StEP filter vial (Restek cat.# 25895). After filtering through the 0.2 µm PVDF membrane, 5 μL was injected for analysis.|
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