NSAIDs on Pinnacle® DB Biphenyl

Peaks
1.Uracil (void marker)
2.Tenoxicam
3.Sulfinpyrazone
image
LC_PH0478A
ColumnPinnacle® DB Biphenyl (cat.# 9409565)
Dimensions:150 mm x 4.6 mm ID
Particle Size:5 µm
Pore Size:140 Å
Temp.:30 °C
Sample
Diluent:0.1% formic acid in water:methanol (40:60)
Conc.:100 µg/mL each component (see peak list)
Inj. Vol.:10 µL
Mobile Phase
A:0.1% formic acid in water
B:methanol
Time (min)%B
060
2.060
8.090
20.090
20.160
Flow:1.0 mL/min
DetectorUV/Vis @ 254 nm
InstrumentShimadzu Prominence
NotesColumn B: phenyl
Column C: phenyl hexyl
Column D: C18
Detector: Shimadzu PDA (SPD-M20A)