Vitamins B1, B2, B3, and B6 on Ultra C8
| Peaks | RT (min) |
| 1. | Vitamin B3 (nicotinamide) | 2.732 |
| 2. | Vitamin B6 (pyridoxine) | 5.615 |
| Peaks | RT (min) |
|---|
| 3. | Vitamin B2 (riboflavin) | 8.719 |
| 4. | Vitamin B1 (thiamine) | 19.004 |
LC_PH0497
| Ultra C8 (cat.# 9103575) |
| 250 mm x 4.6 mm ID |
| 5 µm |
| 100 Å |
|
| 50 °C |
|
| Water:methanol (80:20) |
| 50 µg/mL each component |
| 5 µL |
| 0.008M sodium 1-hexanesulfonate:methanol:glacial acetic acid:triethylamine (817.5:175:7.5:0.2) |
| 2.0 mL/min |
| UV/Vis @ 270 nm |
| Shimadzu UFLCXR |
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