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Clinical, Forensic & Toxicology Solutions

Clinical, Forensic & Toxicology Solutions

Raptor Biphenyl LC Columns (USP L11)

  • Ideal for bioanalytical testing applications like drug and metabolite analyses.
  • Heightened selectivity and retention for compounds that are hard to resolve or elute early on C18 and other phenyl chemistries.
  • Limits ionization suppression and allows simple, MS-friendly mobile phases.
  • Restek’s most popular LC stationary phase applied to 2.7 and 5 µm Raptor SPP core-shell silica.

Rxi-5Sil MS Columns (fused silica)

low-polarity phase; Crossbond 1,4-bis(dimethylsiloxy)phenylene dimethyl polysiloxane

  • Engineered to be a low-bleed GC-MS column.
  • Excellent inertness for active compounds.
  • General-purpose columnsideal for GC-MS analysis of semivolatiles, polycyclic aromatic compounds, chlorinated hydrocarbons, phthalates, phenols, amines, organochlorine pesticides, organophosphorus pesticides, drugs, solvent impurities, and hydrocarbons.
  • Temperature range: -60 °C to 350 °C.

Rtx-BAC Plus 1 Columns (fused silica)

  • Optimized column selectivities guarantee resolution of ethanol, internal standards, and frequently encountered interferences.
  • Robust and reproducible column chemistry ensures longer column lifetime and consistent results.
  • Stable to 260 °C.

Rtx-BAC Plus 2 Columns (fused silica)

  • Optimized column selectivities guarantee resolution of ethanol, internal standards, and frequently encountered interferences.
  • Robust and reproducible column chemistry ensures longer column lifetime and consistent results.
  • Stable to 260 °C.

BAC Resolution Control Standard n-P (6 components)

  • Use to verify the retention time for each compound normally included in a blood alcohol test, and to verify that the compounds are resolved from and do not interfere with one another.
  • Includes 1-propanol internal standard.
  • Intended for qualitative use only.

BAC Resolution Control Standard t-B (6 components)

  • Use to verify the retention time for each compound normally included in a blood alcohol test, and to verify that the compounds are resolved from and do not interfere with one another.
  • Includes tert-butanol internal standard.
  • Intended for qualitative use only.

Pinnacle DB Biphenyl Columns (USP L11)

Particle Size: 1.9 µm, 3 µm, or 5 µm, spherical
Pore Size: 140 Å
Carbon Load: 8%
End-Cap: yes
pH Range: 2.5 to 8
Temperature Limit: 80 °C
USP Phase: L11
Phase Category: phenyl
Ligand Type: unique Biphenyl

Ultra Biphenyl Columns (USP L11)

Particle Size: 3 µm or 5 µm, spherical
Pore Size: 100 Å
Carbon Load: 15%
End-Cap: yes
pH Range: 2.5 to 8
Temperature Limit: 80 °C
USP Phase: L11
Phase Category: phenyl
Ligand Type: unique Biphenyl

Syringe Filters with Luer Lock Inlet

  • Luer lock inlet offers leak-tight syringe connection.
  • Variety of filter types, porosities, and diameters.
  • Color coded for easy identification.
  • Rugged polypropylene housing.
  • Autoclavable to 121 °C for 15 minutes.
  • Quantity break pricing for greater savings.

Forensic Drug Screen Mixes

2.0 mL, 8 mm Autosampler Vial Convenience Kits

Vials packaged in a clear-lid tray. Preassembled caps with septa packaged separately in a plastic bag.

Survival Kits for HPLC

  • Contain a wide range of tubing, fittings, and tools necessary to set up and maintain your HPLC system.
  • Available in stainless steel or PEEK™.

LC/MS in Forensic Toxicology: Selecting a Killer LC Column

An overview of the principles of LC/MS, focusing on the impact of column selection. Attendees will learn how to optimize separations using stationary phase selectivity and column dimensions.
Narrated by Becky Wittrig, Global HPLC Specialist, & Amanda Rigdon, Clinical/Forensics Applications Chemist. Length: 62 minutes

Sample Preparation of Cannabinoids in Urine Using the QuEChERS Method and dSPE Cleanup

This webinar introduces QuEChERS and dSPE and discusses their application to sample prep for GC/MS analysis of cannabinoids in urine. Attendees will learn how to increase sample throughput with this simple approach.
Narrated by . Length: 16 minutes

Technical Articles

Clinical, Forensic & Toxicology

Overcoming the Effects of Highly Organic Protein Precipitation Extracts on LC Peak Shape Using Direct Injection

Protein precipitation is frequently used to minimize matrix impact when analyzing biological samples. However, the effects of highly organic protein precipitation sample extracts on LC peak shape can negatively impact accurate quantification. Dilution or further sample preparation steps are often used to minimize these effects; however, here we show that direct injection of sample extracts is a viable option that can be used to prevent peak distortion, while avoiding the time and variability associated with additional sample preparation.

Clinical, Forensic & Toxicology

High-Throughput Analysis of Immunosuppressive Drugs from Whole Blood by LC-MS/MS

The success of organ transplant therapy depends in large part on the accurate and timely analysis of immunosuppressive drugs. In this study, we developed a fast, accurate method for cyclosporin A, tacrolimus, sirolimus, and everolimus in whole blood. The method pairs a single protein precipitation step with LC-MS/MS analysis using a Raptor Biphenyl column. A fast, 3-minute analysis time was obtained with no interference from matrix components. Excellent results were obtained for linearity, robustness, accuracy, and precision, demonstrating that the method is suitable for high-throughput therapeutic drug monitoring.

Clinical, Forensic & Toxicology

Direct Analysis of Morphine, M3G and M6G Metabolites, and Related Compounds in Urine by LC-MS/MS

The analysis of total morphine is typically conducted by first subjecting urine samples to acid or enzymatic hydrolysis in order to cleave the glucuronide conjugates off of morphine’s primary metabolites [morphine-3β-D-glucuronide (M3G) and morphine-6β-D-glucuronide (M6G)] prior to analysis. With the glucuronide moiety removed, the resulting morphine molecule is much less polar and, therefore, more amenable to traditional reversed-phase chromatography than the metabolites are. However, both hydrolysis procedures cost an analyst time and result in sample variability due to incomplete hydrolysis or analyte conversion. By utilizing the retention characteristics of the Restek Force Biphenyl column, hydrolysis was not required and direct analysis of morphine, its M3G and M6G metabolites, and several related compounds using a simple “dilute-and-shoot” sample preparation was performed.

Bioanalytical

A Rapid and Sensitive LC-MS/MS Method for the Analysis of Three Forms of Thyroid Hormones Using Raptor™ Biphenyl LC Columns

The Raptor Biphenyl column is excellent for rapid and sensitive analysis of thyroid hormones. With the method described here, concentrations of thyroid hormones as low as 2 pg/mL (T3) or 5 pg/mL (T4 and rT3) can be determined with less than 3.5 minutes of total analysis time. The analytical method is thus applicable to the clinical analysis of free thyroid hormone at low pg/mL levels.

Clinical, Forensic & Toxicology

4.5 Minute Analysis of Benzodiazepines in Urine and Whole Blood Using LC/MS/MS and an Ultra Biphenyl Column

Sample throughput for benzodiazepines in urine and whole blood can be increased by adopting this dilute-and-shoot LC/MS/MS method which uses an Ultra Biphenyl HPLC column. Partial validation data are presented in this application note.

Clinical, Forensic & Toxicology

Reduce Downtime and Cost of Materials with Rugged Rxi®-5Sil MS GC Columns

New Rxi®-5Sil MS columns produce consistent results for amphetamine—even after 400 injections of derivatizing reagent—resulting in less time and money spent on column maintenance and replacement.

PDF Document

Rxi-5Sil MS Columns: Assured Performance for Forensic Applications

Rxi-5Sil MS columns produce excellent results for a number of forensic applications. The versatile selectivity separates a wide variety of compounds, which lets you keep analyzing samples instead of changing columns between methods. (PDF - 1751kB)

PDF Document

Fast, Definitive Data for Blood Alcohol Testing

New Rtx®-BAC Plus 1 and Rtx®-BAC Plus 2 columns provide definitive results quickly, so you can maximize sample throughput. These columns baseline separate all critical blood alcohol compounds, including ethanol, methanol, acetone, tert-butanol, acetaldehyde, isopropanol, and n-propanol, in less than 2 minutes. (PDF - 882kB)

PDF Document

New Rtx-BAC Plus 1 and Rtx-BAC Plus 2 Columns: Advanced Technology for Fast, Reliable Measurement of Alcohol in Blood

New Rtx-BAC Plus columns outperform other blood alcohol column pairs and ensure baseline separation of all critical compounds. These columns provide definitive data in a fast, 2-minute analysis, so you can be certain of your results and maximize sample throughput. (PDF - 940kB)

PDF Document

Simplify Sample Prep and Protect Analytical Equipment With Syringe Filters

Filtering sample extracts with syringe filters will greatly extend the life of costly chromatographic columns and replacement parts, particularly for HPLC systems. This 2-page fact sheet includes helpful membrane, porosity, and size selection guides. (PDF - 487kB)

PDF Document

Raptor Biphenyl LC Columns Brochure

Raptor LC columns combine the speed of superficially porous particles (i.e., SPP or “core-shell”) with the resolution of highly selective USLC technology. Featuring Restek's most popular LC stationary phase, the rugged Raptor Biphenyl is extremely useful for fast separations in bioanalytical testing applications like drug and metabolite analyses, especially those that require a mass spectrometer (MS). (PDF - 5848kB)

PDF Document

The Analysis of Vitamin D Metabolites in Serum/Plasma by LC-MS/MS

Separating fat‐soluble vitamins and their metabolites by LC can be quite time consuming, taking up to 20 minutes or longer by some methods. However, the new Raptor™ ARC‐18 LC column can analyze these difficult compounds using reversed phase chromatography (RPC) in much less time, which helps increase sample throughput and overall lab productivity. This technical poster presents methodology that provides excellent quantitative results for metabolites 25‐hydroxyvitamin D2 and 25‐hydroxyvitamin D3 in analysis times of less than 2 minutes. Parent compounds vitamin D2 and vitamin D3 eluted in less than 4 minutes. (PDF - 337kB)