Clinical, Forensic & Toxicology Solutions
A Rapid and Sensitive LC-MS/MS Method for the Analysis of Three Forms of Thyroid Hormones Using Raptor™ Biphenyl LC Columns
The Raptor™ Biphenyl column is excellent for rapid and sensitive analysis of thyroid hormones. With the method described here, concentrations of thyroid hormones as low as 2 pg/mL (T3) or 5 pg/mL (T4 and rT3) can be determined with less than 3.5 minutes of total analysis time. The analytical method is thus applicable to the clinical analysis of free thyroid hormone at low pg/mL levels.
Fast, Robust LC-MS/MS Method for Quantification of Multiple Therapeutic Drug Classes Using an Ultra Biphenyl Column
Therapeutic drug monitoring requires streamlined, cost-effective testing procedures. This article details a fast, robust LC-MS/MS method for the quantification of 29 therapeutic drugs and metabolites in urine from several classes including opiates, benzodiazepines, tricyclic antidepressants, and anticonvulsants. Good linearity, accuracy, and precision results were obtained for most analytes in a fast, 5.5-minute analysis.
This application note details a fast extraction and analysis method for a wide range of metabolites of synthetic cannabinoids JWH-018 and JWH-073 in urine. Quantitative results are reported for carboxylated and mono-hydroxylated metabolites, including positional isomers.
4.5 Minute Analysis of Benzodiazepines in Urine and Whole Blood Using LC/MS/MS and an Ultra Biphenyl Column
Sample throughput for benzodiazepines in urine and whole blood can be increased by adopting this dilute-and-shoot LC/MS/MS method which uses an Ultra Biphenyl HPLC column. Partial validation data are presented in this application note.
New Rxi®-5Sil MS columns produce consistent results for amphetamine—even after 400 injections of derivatizing reagent—resulting in less time and money spent on column maintenance and replacement.
Rxi-5Sil MS columns produce excellent results for a number of forensic applications. The versatile selectivity separates a wide variety of compounds, which lets you keep analyzing samples instead of changing columns between methods. (PDF - 1751kB)
Versatile Biphenyl columns provide excellent retention of both polar and nonpolar compounds, resulting in improved resolution of benzodiazepines, cannabinoids, and other key target compounds for clinical or forensic applications. (PDF - 633kB)
New Rtx®-BAC Plus 1 and Rtx®-BAC Plus 2 columns provide definitive results quickly, so you can maximize sample throughput. These columns baseline separate all critical blood alcohol compounds, including ethanol, methanol, acetone, tert-butanol, acetaldehyde, isopropanol, and n-propanol, in less than 2 minutes. (PDF - 522kB)
New Rtx-BAC Plus 1 and Rtx-BAC Plus 2 Columns: Advanced Technology for Fast, Reliable Measurement of Alcohol in Blood
New Rtx-BAC Plus columns outperform other blood alcohol column pairs and ensure baseline separation of all critical compounds. These columns provide definitive data in a fast, 2-minute analysis, so you can be certain of your results and maximize sample throughput. (PDF - 940kB)
Filtering sample extracts with syringe filters will greatly extend the life of costly chromatographic columns and replacement parts, particularly for HPLC systems. This 2-page fact sheet includes helpful membrane, porosity, and size selection guides. (PDF - 600kB)
Raptor LC columns combine the speed of superficially porous particles (i.e., SPP or “core-shell”) with the resolution of highly selective USLC technology. Featuring Restek's most popular LC stationary phase, the rugged Raptor Biphenyl is extremely useful for fast separations in bioanalytical testing applications like drug and metabolite analyses, especially those that require a mass spectrometer (MS). (PDF - 5848kB)
Separating fat‐soluble vitamins and their metabolites by LC can be quite time consuming, taking up to 20 minutes or longer by some methods. However, the new Raptor™ ARC‐18 LC column can analyze these difficult compounds using reversed phase chromatography (RPC) in much less time, which helps increase sample throughput and overall lab productivity. This technical poster presents methodology that provides excellent quantitative results for metabolites 25‐hydroxyvitamin D2 and 25‐hydroxyvitamin D3 in analysis times of less than 2 minutes. Parent compounds vitamin D2 and vitamin D3 eluted in less than 4 minutes. (PDF - 337kB)