Comprehensive LC-MS/MS Analysis of 15 Bisphenols in 8 Minutes

Published By: Restek Corporation

Year of Publication: 2018

Link: http://www.restek.com/Technical-Resources/Technical-Library/Foods-Flavors-Fragrances/fff_FFSS2935-UNV

Abstract: The analysis of bisphenolic compounds that are used as alternatives to bisphenol A (BPA) is essential for monitoring exposure to and evaluating the safety of these emerging industrial chemicals. The LC-MS/MS method presented here provides excellent chromatographic peak shape and separation for an extensive list of BPA analogues in a fast, 8-minute analysis.

Fast, Simple LC-MS/MS Analysis of Creatine and Creatinine

Published By: Restek Corporation

Year of Publication: 2018

Link: http://www.restek.com/Technical-Resources/Technical-Library/Clinical-Forensic-Toxicology/cft_CFSS2937-UNV

Abstract: A rapid, robust method was established for the analysis of creatine and creatinine in human plasma and urine. An efficient separation was achieved in less than 1.5 min using a Raptor HILIC-Si column and a simple isocratic mobile phase.

When Do We Need Sub2m Superficially Porous Particles for Liquid Chromatography Separations

Author(s): David Bell, Landon Wiest, Shun-Hsin Liang, Dan Li
Restek Corporation

Published By: LCGC Asia Pacific

Issue: Volume 21, Issue 3

Year of Publication: 2018

Link: http://www.chromatographyonline.com/when-do-we-need-sub2m-superficially-porous-particles-liquid-chromatography-separations

Abstract: The use of superficially porous particles (SPPs) for modern high performance liquid chromatography (HPLC) is now very common. Initially, SPPs rose as an alternative to sub-2-µm fully porous particles (FPPs). In recent years, many column manufacturers have developed 2-µm and smaller SPP-based products. This article investigates the practical utility of these smaller SPP designs.

LC–MS Sensitivity: Practical Strategies to Boost Your Signal and Lower Your Noise

Author(s): Sharon Lupo
Restek Corporation

Published By: LCGC North America

Issue: Volume 36, Issue 9

Year of Publication: 2018

Link: http://files.pharmtech.com/alfresco_images/pharma/2018/09/20/34989658-b89e-46ca-9d30-f7577e2ef6c9/LCGC_NAmerica_Sept2018.pdf#page=14

Abstract: Liquid chromatography–mass spectrometry (LC–MS) has become the preferred analytical technique for many challenging assays based on its selectivity, sensitivity, and broad applicability to compounds of varying polarity. Despite the advantages of the technique, the complexity of LC–MS systems often leaves analysts struggling to meet method detection limits. In this installment of “Column Watch,” several strategies are discussed to improve method sensitivity through the reduction of contaminants, the careful selection of LC method conditions, and the optimization
of MS interface settings. By understanding the relationship between these parameters and ionization efficiency, analysts can enhance their signal-to-noise ratio and realize the hidden potential of the LC–MS technique.

A Novel Solution for Vitamin K1 and K2 Analysis in Human Plasma by LC-MS/MS

Author: Shun-Hsin Liang and Frances Carroll

Published By: Restek Corporation

Year of Publication: 2018

Link: http://www.restek.com/Technical-Resources/Technical-Library/Clinical-Forensic-Toxicology/cft_CFAN2934-UNV

Abstract: Vitamin K1 and K2 analysis is typically complex and time-consuming because these lipophilic vitamins occur at low levels and are subject to matrix interference, particularly from phospholipids. The new method established here provides fast, accurate, and precise vitamin K levels in plasma using a simple phospholipid removal procedure and a sensitive 4-minute LC-MS/MS analysis on a Raptor Biphenyl column, making it suitable for high-throughput testing.Quantitative analysis of bile acids in plasma is critical for diagnosing liver disease as well as assessing drug safety. Accurate reporting can be difficult because of analyte characteristics, matrix effects, and other factors. Here, we establish a rapid, robust, and selective LC-MS/MS method for the analysis of bile acids in human plasma using a Restek Raptor C18 column. One of the significant improvements over other reported methods was the baseline separation of all 17 bile acids in 6 minutes including three isomer groups.

High-Throughput Analysis of Mycotoxins in Cannabis CBD Oil Pairs Simplified Cleanup with LC-MS/MS Sensitivity

Published By: Restek Corporation

Year of Publication: 2018

Link: http://www.restek.com/Technical-Resources/Technical-Library/Foods-Flavors-Fragrances/fff_FFSS2946-UNV

Abstract: Complex, time-consuming immunoaffinity column sample prep protocols for mycotoxins can be replaced by this simple pass-through SPE sample cleanup. Paired with LC-MS/MS analysis using a Raptor Biphenyl column, excellent chromatographic results were obtained for aflatoxins and ochratoxins at 2 ng/g, providing a high-throughput solution for analyzing mycotoxins in cannabis.

High-Resolution GC Analyses of Fatty Acid Methyl Esters (FAMEs)

Published By: Restek Corporation

Year of Publication: 2018

Link: http://www.restek.com/Technical-Resources/Technical-Library/Foods-Flavors-Fragrances/fff_FFAR2931-UNV

Abstract: Capillary GC is especially useful for determining total fat content, trans fat content, and total omega-3 polyunsaturated fatty acid content in foods. The choice of capillary column depends on the information required. As shown here, whatever your fatty acid analysis requirements, Restek can provide the consistent-performance analytical columns and reference mixes that will help you to accurately characterize your materials.

High-Throughput LC-MS/MS Analysis of Phosphatidylethanol in Whole Blood

Published By: Restek Corporation

Year of Publication: 2018

Link: http://www.restek.com/Technical-Resources/Technical-Library/Clinical-Forensic-Toxicology/cft_CFSS2936-UNV

Abstract: Accurate analysis of phosphatidylethanol (PEth) can be achieved with a simple protein precipitation procedure and a fast 3.5 minute LC-MS/MS gradient run. The method shown here utilizes a Raptor FluoroPhenyl column and provides a low-cost, high-throughput solution for labs monitoring PEth as a biomarker for alcohol consumption.

Rapid Analysis of 17 Bile Acids in Human Plasma by LC-MS/MS

Author: Dan Li and Frances Carroll

Published By: Restek Corporation

Year of Publication: 2018

Link: http://www.restek.com/Technical-Resources/Technical-Library/Clinical-Forensic-Toxicology/cft_CFAN2911-UNV

Abstract: Quantitative analysis of bile acids in plasma is critical for diagnosing liver disease as well as assessing drug safety. Accurate reporting can be difficult because of analyte characteristics, matrix effects, and other factors. Here, we establish a rapid, robust, and selective LC-MS/MS method for the analysis of bile acids in human plasma using a Restek Raptor C18 column. One of the significant improvements over other reported methods was the baseline separation of all 17 bile acids in 6 minutes including three isomer groups.

7.5-Min Screening Analysis of Multiresidue Pesticides in Brown Rice Flour

Published By: Restek Corporation

Year of Publication: 2018

Link: http://www.restek.com/Technical-Resources/Technical-Library/Foods-Flavors-Fragrances/fff_FFSS2930-UNV

Abstract: Multiresidue pesticides analysis presents many challenges, from extensive lists of chemically diverse analytes to the negative effects of complex matrix components. The optimized chromatographic method described here allows the analysis of hundreds of compounds in as little as 7.5 minutes with a total cycle time under 10 minutes, ensuring fast, accurate sample screening and increased lab productivity.