LC-MS/MS Analysis of Urinary Free Cortisol and Cortisone without Matrix Interferences

Published By: Restek Corporation

Year of Publication: 2018

Link: http://www.restek.com/Technical-Resources/Technical-Library/Clinical-Forensic-Toxicology/cft_CFSS2824-UNV

Abstract: 

In general, LC-MS/MS analysis of urinary free cortisol and cortisone can provide better specificity than immunoassay-based methods. However, it is critical that the analytical column has the proper selectivity for chromatographically separating cortisol from isobaric matrix components. In the method presented here, complete separation of the target analytes and matrix interferences was obtained in a 3-minute analysis on a Raptor Biphenyl column.