delim
Roc HPLC Columns

NEW! Roc HPLC Columns:
The Reliable Cornerstone
for Your LC Lab


  • Solid and reliable performance.
  • Exceptional value.
  • 5 phases covering 98% of USP methods.
  • Minimal packaging for compact storage.

Solid, reliable Roc HPLC columns are pressure rated for any 400 bar HPLC system and deliver the peak shape, reproducibility, ruggedness, and performance you demand for all of your conventional HPLC applications, especially USP and other compendial methods. An exceptional value for routine analyses and large-volume workflows, Roc columns are made from high-purity, fully porous silica backed by quality manufacturing and are available in a variety of phases. Just 5 phases cover 98% of USP methods. And newly designed minimal packaging offers compact storage with serial #-specific certificates of analysis available for download at www.restek.com/documentation

Get more of what you want, and less of what you don't, with Roc LC columns from Restek.


The Roc Family of HPLC Phases:


C18

C8

Phenyl-Hexyl

Cyano

Silica

USP Phase Code

L1

L7

L11

L10

L3

Stationary Phase Category

C18, octadecylsilane

C8, octylsilane

phenyl

cyano

bare silica

Ligand Type

monomeric C18

monomeric C8

phenyl-hexyl

cyanopropyl

n/a

Particle Size

3 μm or 5 μm, spherical

3 μm or 5 μm, spherical

3 μm or 5 μm, spherical

3 μm or 5 μm, spherical

3 μm or 5 μm, spherical

Pore Size

100 Å

100 Å

100 Å

100 Å

100 Å

Surface Area

300 m2/g

300 m2/g

300 m2/g

300 m2/g

300 m2/g

Carbon Load

20%

12%

15%

8%

n/a

End-Cap

yes

yes

yes

yes

n/a

pH Range

2.5 to 8.0

2.5 to 8.0

2.0 to 8.0

2.5 to 8.0

2.5 to 8.0

Maximum Temperature

80 °C

80 °C

80 °C

80 °C

80 °C



Roc LC Columns from Restek Perform Better Than or Equal To Columns from Other Vendors

Table I: Roc LC columns offer equivalent or better peak shape, retention, and resolution compared to FPP columns from other manufacturers under identical conditions (average values, n = 5).

Columns

Analyte

tR

Area

Height

USP Tailing

Asymmetry (10%)

k'

Plate Number

Resolution

Roc

Ethylparaben

3.252

1,407,609

281,358

1.176

1.148

1.182

9,738

12.5

Ethinyl Estradiol

5.410

1,869,684

234,346

1.075

1.064

2.631

10,264

Vendor A

Ethylparaben

3.060

1,424,621

215,698

1.907

1.608

1.013

6,732

10.1

Ethinyl Estradiol

4.889

1,860,659

223,879

1.311

1.217

2.217

8,575

Vendor B

Ethylparaben

2.873

1,426,068

319,850

1.143

1.124

0.866

9,706

9.9

Ethinyl Estradiol

4.307

1,867,049

287,414

1.064

1.057

1.797

10,031

Vendor C

Ethylparaben

2.763

1,413,099

315,080

1.262

1.217

0.932

8,643

11.9

Ethinyl Estradiol

4.580

1,868,724

270,662

1.143

1.122

2.203

9,700

Vendor D

Ethylparaben

3.157

2,939,187

357,541

1.159

0.965

1.084

4,478

12.2

Ethinyl Estradiol

5.816

1,771,785

182,056

1.301

1.232

2.839

8,954

Vendor E

Ethylparaben

3.873

1,498,446

244,642

1.172

1.134

1.221

9,348

13.7

Ethinyl Estradiol

6.839

1,781,453

174,955

1.080

1.063

2.921

9,963



Figure 1: Count on Roc LC columns from Restek for outstanding chromatographic performance.

Roc® LC Column

PeakstR (min)Conc.
(µg/mL)
1.Ethylparaben3.25250
2.Ethinyl estradiol5.410200
Ethinyl Estradiol on Roc® C18 - USP Method
LC_EX0518

Vendor A

PeakstR (min)Conc.
(µg/mL)
1.Ethylparaben3.06050
2.Ethinyl estradiol4.889200
Ethinyl Estradiol on Vendor A C18 - USP Method
LC_EX0521

Vendor B

PeakstR (min)Conc.
(µg/mL)
1.Ethylparaben2.87350
2.Ethinyl estradiol4.307200
Ethinyl Estradiol on Vendor B C18 - USP Method
LC_EX0520

Vendor C

PeakstR (min)Conc.
(µg/mL)
1.Ethylparaben2.76350
2.Ethinyl estradiol4.580200
Ethinyl Estradiol on Vendor C C18 - USP Method
LC_EX0519

Vendor D

PeakstR (min)Conc.
(µg/mL)
1.Ethylparaben3.15750
2.Ethinyl estradiol5.816200
Ethinyl Estradiol on Vendor D C18 - USP Method
LC_EX0522

Vendor E

PeakstR (min)Conc.
(µg/mL)
1.Ethylparaben3.87350
2.Ethinyl estradiol6.839200
Ethinyl Estradiol on Vendor E C18 - USP Method
LC_EX0523

Chromatographic conditions for Table I and Figure 1
All columns are C18 stationary phase (5 µm, 150 x 4.6 mm). Conditions are as follows. Mobile phase: water:acetonitrile (1:1); Flow rate: 1 mL/min; Injection volume: 25 µL; Instrument: HPLC with UV detector, 280 nm; Sample: 200 µg/mL ethinyl estradiol and 50 µg/mL ethylparaben in water:acetonitrile (1:1).

Columns

Roc C18 HPLC Columns (USP L1)

  • Conventional HPLC column built to be the cornerstone for your lab; pressure rated for any 400 bar HPLC system.
  • Solid and reliabledelivers the peak shape, reproducibility, ruggedness, and performance you demand.
  • Exceptional value for routine analyses and large-volume workflowshigh-purity, fully porous silica backed by quality manufacturing features a variety of phases to fit most HPLC methods.
  • Ideal for a wide range of applications from food to pharma;
    5 phases cover 98% of USP methods.

Roc C8 HPLC Columns (USP L7)

  • Conventional HPLC column built to be the cornerstone for your lab; pressure rated for any 400 bar HPLC system.
  • Solid and reliabledelivers the peak shape, reproducibility, ruggedness, and performance you demand.
  • Exceptional value for routine analyses and large-volume workflowshigh-purity, fully porous silica backed by quality manufacturing features a variety of phases to fit most HPLC methods.
  • Ideal for a wide range of applications from food to pharma;
    5 phases cover 98% of USP methods.

Roc Phenyl-Hexyl HPLC Columns (USP L11)

  • Conventional HPLC column built to be the cornerstone for your lab; pressure rated for any 400 bar HPLC system.
  • Solid and reliabledelivers the peak shape, reproducibility, ruggedness, and performance you demand.
  • Exceptional value for routine analyses and large-volume workflowshigh-purity, fully porous silica backed by quality manufacturing features a variety of phases to fit most HPLC methods.
  • Ideal for a wide range of applications from food to pharma;
    5 phases cover 98% of USP methods.

Roc Cyano HPLC Columns (USP L10)

  • Conventional HPLC column built to be the cornerstone for your lab; pressure rated for any 400 bar HPLC system.
  • Solid and reliabledelivers the peak shape, reproducibility, ruggedness, and performance you demand.
  • Exceptional value for routine analyses and large-volume workflowshigh-purity, fully porous silica backed by quality manufacturing features a variety of phases to fit most HPLC methods.
  • Ideal for a wide range of applications from food to pharma;
    5 phases cover 98% of USP methods.

Roc Silica HPLC Columns (USP L3)

  • Conventional HPLC column built to be the cornerstone for your lab; pressure rated for any 400 bar HPLC system.
  • Solid and reliabledelivers the peak shape, reproducibility, ruggedness, and performance you demand.
  • Exceptional value for routine analyses and large-volume workflowshigh-purity, fully porous silica backed by quality manufacturing features a variety of phases to fit most HPLC methods.
  • Ideal for a wide range of applications from food to pharma;
    5 phases cover 98% of USP methods.

Supplies & Accessories

Roc LC Guard Column Cartridges

  • Protect your Roc LC columns with minimal effect on retention, peak shape, or efficiency.

Roc LC Guard Column Holder

  • Protect your Roc LC columns with minimal effect on retention, peak shape, or efficiency.
  • Requires separate guard column cartridges (available from Restek).

Replacement PEEK Ferrules for Roc LC Guard Column Holder

Replacement PEEK ferrules for use with the Roc LC guard column holder (sold separately).
PDF Document

Roc HPLC Columns: The Reliable Cornerstone for Your LC Lab

Solid, reliable Roc HPLC columns deliver the peak shape, reproducibility, ruggedness, and performance you demand for all of your conventional HPLC applications. Available in a variety of phases, Roc columns are made from high-purity silica and feature minimal packaging with serial #-specific certificates of analysis available for download. (PDF - 2MB)