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Trident LC Column Protection System

Redesigned to be more rugged and easier to use!

... at http://www.restek.com/Pages/faq_lc...

Resources

ProFLOW 6000 Electronic Flowmeter: Frequently Asked Questions (FAQ) (PDF)

...Frequently Asked Questions (FAQ) ProFLOW 6000 Electronic Flowmeter 1. Why do I see and repair. 14. Where can I learn more about flow regimes than this simple FAQ provides? The following ... , standard temperature and pressure, ccm, sccm, flow compensation, flowmeter calibration expired; gas flow reading, FAQ, flowmeter FAQ...

Frequently Asked Questions: Surface Treatments

...frequently asked questions, faqs, faq...

Frequently Asked Questions: Capillary GC Columns

...frequently asked questions, faqs, faq...

Frequently Asked Questions: Packed & Micropacked Columns

...frequently asked questions, faqs, faq...

Frequently Asked Questions (FAQs): Reference Standards

...frequently asked questions, faqs, faq...

Frequently Asked Questions: Air Sampling

... and treated products, refer to our surface treatment FAQs ... frequently asked questions, faqs, faq...

Frequently Asked Questions: GC Accessories

...frequently asked questions, faqs, faq...

Restek Trident LC Column Protection System Instruction Sheet (PDF)

...-Restek column connections. A detailed discussion about port configurations can be found at http://www.restek.com/Pages/faq...

Frequently Asked Questions: Sample Preparation

Frequently Asked Questions: Sample Preparation FAQ

ProFLOW 6000 Electronic Flowmeter—Frequently Asked Questions (FAQ)

... learn more about flow regimes than this simple FAQ provides Where can I learn more about flow regimes than this simple FAQ provides? Restek ... flowmeter FAQ page. ... , standard temperature and pressure, ccm, sccm, flow compensation, flowmeter calibration expired; gas flow reading, FAQ, flowmeter FAQ...

Trident LC Column Protection System: Redesigned to be more rugged and easier to use! (PDF)

... connections. A detailed discussion about port configurations can be found at http://www.restek.com/Pages/faq...

ChromaBLOGraphy: Choosing a guard cartridge holder for LC

If you need help choosing one of the Trident guard cartridge holders, check out our information found in the HPLC and UHPLC FAQs section of our website. The FAQs contain a lot of good information and are often overlooked. For most of our columns we prefer the Trident Direct Guard holder system, because of its […]

Frequently Asked Questions: HPLC and UHPLC

... injection (see FAQ #8 above). Keep in mind that your sample components must be soluble in mobile phase ). Please see our earlier FAQ regarding the advantages of SPP ... to this and other FAQs on Restek’s frequently asked questions page for LC guard cartridges....

Flip Seal Inlet Seals Frequently Asked Questions

...—flip—then use again. Review our Flip Seal FAQ page for quick answers on how to install and optimize use...

ChromaBLOGraphy: Finding the right fitting for HPLC or UHPLC

If you are wondering which type of fitting you need, you can find a wealth of information in the Frequently Asked Questions (FAQs) section of our website, under the HPLC and UHPLC category. Click on the links below for answers to some common questions: What size threads are on the end fittings of my HPLC […]

ChromaBLOGraphy: Contents inside your baseplate trap

We often get asked in tech service for a list of materials contained inside our baseplate traps from customers who are concerned about disposal regulations.  They have read our FAQ How do I dispose of used gas traps or filters?, but before contacting their waste disposal company, a list of the trap contents is requested.  […]

ChromaBLOGraphy: Things to Consider Before Ordering a Packed Column

Let’s say you are starting your next GC project and realize that it is a packed column method.  However, you normally use capillary columns, and are not familiar with what you need to know before you order a new packed column. Believe me when I say “you are not alone.” Hopefully this post will help [...]

Diagnosing and Preventing High Back Pressure Problems in LC Systems (PDF)

... at http://www.restek.com/Pages/faq_lc#top Filtration 14 www.restek.com Description Similar to Agilent...

ChromaBLOGraphy: Choosing a guard cartridge for LC

So you have a guard cartridge holder of some sort, and you don’t have the guard cartridge to put inside. If you happen to have missed that last blog post about the holder, click on the following link. Choosing a guard cartridge holder for LC Let’s determine what type of guard cartridge you need. A […]

ChromaBLOGraphy: Packed Column information for the beginner

We seem to be getting more calls from first time users of packed columns, so I decided to write this post for these beginners to help them understand the terminology commonly used when describing these products.   What is a packed column? Unlike GC capillary columns, which are referred to as WCOT (Wall Coated Open […]

ChromaBLOGraphy: Looking for a quote for a GC custom packed column?

To check to see if we can manufacture a custom packed column, you will need to complete the form found in the link below. If we can manufacture the column, you will automatically receive a quote for it.  If not, customer service will inform you and let you know why. Custom Packed/Micropacked Column Request Form […]

ChromaBLOGraphy: How can I use Raptor columns and the EXP Direct connect holder with stainless steel fittings?

Some customers may find themselves with this scenario. You have an HPLC instrument with existing stainless steel tubing and fittings and you are getting ready to install a Raptor column. The end of your tubing may very well look like the one shown above. Here are some questions you might have.   Can I attach […]

ChromaBLOGraphy: Certified Reference Material Explained

Restek offers Certified Reference Material (CRM) for a wide variety of analytical methods. But what makes a reference material certified and are there different certifications available? Let’s start with a brief overview of ISO accreditation. A company is first registered to standards such as ISO 9001:2008, which indicates they have an approved quality system in […]

ChromaBLOGraphy: Changes are coming to the MSDS; um, I mean the SDS

You may have noticed that our MSDSs (Material Safety Data Sheets) are now called SDSs (Safety Data Sheets).  In 2012, OSHA revised The Hazard Communication Standard (HCS) (29 CFR 1910.1200(g)).  The purpose of the form is the same; however, it is expected that each form now be written in a clear, consistent, 16-section format.  For [...]

ChromaBLOGraphy: [3]Troubleshooting HPLC- Tailing Peaks

Patterns in your HPLC chromatography can exhibit telltale signs that point toward probable sources of error.  In this brief series of posts, we will look at possible scenarios that you may encounter in the laboratory and how you might approach resolving those difficulties. If you are having difficulty with tailing, it is likely that you […]

ChromaBLOGraphy: How Do I Scale Up to a Prep LC Column?

  As mentioned in my previous post What is Different about Prep LC?, we ask that you start by developing your method on an analytical scale column. Once you have accomplished this, and wish to start using prep scale HPLC for purification, you will need to determine what size preparative scale (“prep”) column would be […]

ChromaBLOGraphy: What is SPP and when should I use a Raptor column?

While this sounds like a very broad question, I’m sure it is crossing some of your minds. To start out, SPP stands for superficially porous particles. It has also been called “core-shell”.   If you are a veteran in the world of HPLC, this term is old hat for you. If you are new to the […]

ChromaBLOGraphy: Molecular Sieve 5A & 13X packed columns – Installation / Conditioning / Helpful Hints

Molecular Sieve 5A & 13X Columns (packed) Molecular Sieve 5A & 13X (micropacked) Things to Consider Before Ordering a Packed Column . Following the steps below will ensure that your Molecular Sieve packed column is ready to use just several hours after installation.  You should only use dry high-purity Nitrogen as the carrier gas to […]

ChromaBLOGraphy: Capillary GC Column Killers – Part 4

Aqueous Injections In my final installment of Capillary GC Column Killers, I would like to discuss aqueous (water) injections. There are a lot of misconceptions about injecting water into a GC, and for good reason. Besides the general knowledge that electronics and water should not be combined, many analysts have experienced chromatography issues when injecting [...]

ChromaBLOGraphy: Need some help choosing a hydrogen generator?

As customers continue to switch to gas generators as their main source for hydrogen, we often get asked for assistance to help them make the right choice.  As a result, I decided to write this post to help our customers in their selection.   . In most cases, choosing the appropriate generator is as simple […]

ChromaBLOGraphy: Do reverse phase HPLC columns really need conditioning?

  Well, it’s partly a matter of semantics sometimes, but there is usually nothing drastic that you need to do to get your HPLC column going if it is new.  There is no baking at high temperatures involved like you might do for GC or that sort of thing. You do, however, need to equilibrate […]

ChromaBLOGraphy: Think twice before purchasing a 3/16” OD packed column

So you may be asking yourself, why the cautionary statement? Well, it’s very simple, many of our customers who purchase 3/16” OD (outside diameter) packed columns have a difficult time installing them into their GC oven.  Why?  Sometimes finding the appropriate fitting/ferrule is difficult. Let’s say you have a packed column instrument. If your injection port […]

ChromaBLOGraphy: [4]Troubleshooting HPLC- Fronting Peaks

Patterns in your HPLC chromatography can exhibit telltale signs that point toward probable sources of error.  In this brief series of posts, we have looked at possible scenarios that you may encounter in the laboratory and how you might approach resolving those difficulties. Any time you have difficulty with poor peak shape, it is also […]

ChromaBLOGraphy: Troubleshooting HPLC- Loss in Response for All Analytes

[1]Troubleshooting HPLC- Loss in Response for all Analytes Just as in GC applications, patterns in your HPLC chromatography can exhibit telltale signs that point toward probable sources of error.  In this brief series of posts, we will look at possible scenarios that you may encounter in the laboratory and how you might approach resolving those […]

ChromaBLOGraphy: ShinCarbon columns – Installation / Conditioning / Helpful Hints

For those of you who may be interested in using a ShinCarbon for the first time, I’ll walk you through the process of getting your new column ready for analysis. Even though we take great care to minimize the time you will need to condition your column after you receive it, please remember, this packing, like all molecular sieves, likes to absorb [...]

ChromaBLOGraphy: “How Long Will a GC Column Last?”

Once of the most common questions in technical support is “how long will my GC column last” or perhaps “what is a typical lifetime for a GC column.” Unfortunately, these are very difficult questions for which there are no definitive answers. Why? Because column lifetime always depends on a wide range of variables such as… […]

ChromaBLOGraphy: Calculating pH

I’m guessing that most of us that work in the lab would find it more convenient to measure a pH rather than to calculate a theoretical value.  But, at times, it can be very useful to make that calculation and get an idea of what to expect.  As far as chromatography is concerned, all columns […]

ChromaBLOGraphy: How to choose the correct GC column – Part 4

In Parts 1-3, I explained the process I use to help select the correct GC column. Hopefully it has provided a foundation of how someone may go about choosing an appropriate column for a particular compound, or group of compounds. For the fourth and final post in my series, I would like to present a [...]

ChromaBLOGraphy: [2]Troubleshooting HPLC- Loss in Response for Some, but Not All Analytes

[2]Troubleshooting HPLC- Loss in Response for Some, but Not All Analytes Patterns in your HPLC chromatography can exhibit telltale signs that point toward probable sources of error.  In this brief series of posts, we will look at possible scenarios that you may encounter in the laboratory and how you might approach resolving those difficulties. When […]

ChromaBLOGraphy: Live Long and Prosper-How to make your HPLC columns last longer

We frequently get questions about how long an HPLC or UHPLC column should last. To borrow a phrase from my colleagues, “Your mileage may vary.”  Columns are like cars, so their lifetime depends how they are used and how well they are maintained. That being said, some phases are known to be more rugged than [...]

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