share:
delim
delim
Emerald 2020

Emerald Conference 2020

Restek at Emerald 2020
Restek at Emerald 2020

ORAL SESSION:

Analysis of the California List of Pesticides and Mycotoxins in Edibles
Nathaly Reyes (presenter)
Restek Corporation
For more information, email Nathaly Reyes.
Read abstract

Legalization of cannabis for recreational and medical purposes demands reliable analytical methodologies to assess the quality of any cannabis-derived product. Depending on the country or state, different testing requirements apply depending on the product type. Among the various commercially available products infused with cannabinoids, edibles constitute one of the most popular categories. Edibles encompass a broad variety of products including, among others, several types of beverages, chocolates, baked goods and candy. Currently, the state of California requires the determination of mycotoxins and pesticides not only in cannabis flower, but also in cannabis-derived goods. For this reason, the development of analytical methodologies capable of tackling the challenges associated with different matrices are highly desired. This work describes an effective workflow for the simultaneous analysis of the California list of pesticides and mycotoxins in brownies with only 3 mL of solvent consumed per sample, using both LC-MS/MS and GC-MS/MS for instrumental analysis. Multiple experimental parameters were evaluated and valuable lessons were learned over the course of the sample preparation optimization. For instance, it was found that the addition of acetic acid to the extraction solvent enhanced the recovery of daminozide by approximately 5-fold. In addition, enhancements in recovery values of about 20% were observed for several analytes by performing a two-step extraction compared to a typical single-step extraction. The use of internal standards and matrix matched calibration were imperative to obtain a reliable methodology. Overall, the proposed workflow provided excellent results in terms of linearity, accuracy, precision, and limits of quantitation (LOQs).

RESTEK TECHNICAL POSTERS

Evaluation of Injection Techniques for Analyzing Terpenes
Colton Myers (presenter)
Restek Corporation
For more information, email Colton Myers.
Download a PDF of the full presentation.
Read abstract

Terpenes are a class of organic compounds that are comprised of isoprene units based on 5 carbons. These compounds are produced by a variety of plants and generally have a strong odor. Different terpenes, and ratios of terpenes, can be found in different cannabis chemovars. Not only are terpenes identified in cannabis for their aromas and flavors, but they may also play a major role in the entourage effect. The entourage effect is believed to be created by the synergistic interaction between cannabinoids, terpenes, and other naturally occurring compounds, which gives each cannabis chemovar its own unique experience. This research is focused on evaluating injection techniques for analyzing terpenes via gas chromatography–mass spectrometry (GC-MS). These injection techniques include Headspace-Syringe (HS-Syringe), HS-Solid Phase Microextraction (HS-SPME), Direct Immersion-SPME (DI-SPME), and Liquid Injection (LI).

The LC-UV Analysis of 19 Cannabinoids of Interest in Cannabis Products
Nathaly Reyes (presenter)
Restek Corporation
For more information, email Nathaly Reyes.
Download a PDF of the full presentation.
Read abstract

More than 100 cannabinoids have been isolated from cannabis in addition to the five most commonly tested: THC, THCA, CBD, CBDA, and CBN. While methods have been published that show the separation of these major cannabinoids, many do not take into account the possibility of interference from other cannabinoids that may be present. This is most problematic in concentrates where minor cannabinoids can be enriched to detectable levels that were not observed in the flower. In this study, the LC-UV separation of 19 cannabinoids of interest was performed while monitoring for the potential impact from minor cannabinoids. The method was originally developed on a Raptor ARC-18 150 x 4.6 mm, 2.7 µm column, but was also translated to two other column dimensions: 150 x 2.1 mm, 2.7 µm and 100 x 3.0 mm, 1.8 µm. The ability to use multiple column dimensions enables a fit-for-purpose method that can highlight one or more of the following benefits: reduced solvent consumption, increased sample throughput, MS compatibility, increased resolution, or increased sensitivity. The method was applied to cannabis products such as concentrates, oils, edibles, and personal care products.

Visit the Emerald Conference 2020 website

Restek Domestic Customer Service

Subject

Message

Your Full Name

Your Email

Company Name

Address

Spam Block (Please leave this blank)
Drag and Drop Attachments Here (optional)
...


all fields required

Thank you

Your message has been sent. We will be in touch shortly.

Message not sent

Sorry, your message could not be sent at this time. Please try again later, or contact Restek or your local Restek representative via phone.

www.restek.com/Contact-Us