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MSACL 2018 EU

Mass Spectrometry: Applications to the Clinical Lab, 5th Annual European Congress and Exhibition

Restek at MSACL EU 2018
Restek at MSACL EU 2018

RESTEK TECHNICAL POSTERS

Analysis of Phosphatidylethanol in Human Whole Blood by LC-MS/MS
Shun-Hsin Liang, Ravali Alagandula, Frances Carroll (presenter), Justin Steimling, Sue Steinike, Paul Connolly
Restek Corporation
For more information, email Frances Carroll.
Download a PDF of the full presentation.
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Phosphatidylethanol (PEth) is a group of phospholipids formed through enzymatic reaction between ethanol and phosphatidylcholine on the cell membrane. Among multiple homologues of PEth, PEth-16:0/18:1 (palmitic acid/oleic acid) is the predominant molecule extracted from human erythrocytes and can be measured in whole blood as specific biomarker of alcohol consumption with a detection window of up to 3–4 weeks and with the application of highly sensitive LC-MS/MS techniques, it is now possible to use PEth concentration in blood to differentiate chronic drinking from social drinking or as a marker of absolute abstinence. In this study, a fast chromatographic analysis was developed using a Raptor FluoroPhenyl column. Specific and sensitive measurement of PEth-16:0/18:1 in whole blood was achieved with a combination of simple protein precipitation and fast 3.5-minute LC cycle time.

A Novel Solution for Vitamin K1 and K2 Analysis in Human Plasma by LC-MS/MS
Shun-Hsin Liang, Ravali Alagandula, Frances Carroll, Justin Ty Kahler, Sue Steinike, Paul Connolly, Hansjoerg Majer (presenter)
Restek Corporation
For more information, email Hansjoerg Majer.
Download a PDF of the full presentation.
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Vitamin K is a group of fat-soluble vitamins divided into vitamin K1 (one compound, phylloquinone) and K2 (a group of compounds, menaquinones). As an interest for their biological action in extra-hepatic tissues is increasing, an accurate and simple measurement of vitamin K status remains a critical issue for both clinical research and diagnostics. There is a high degree of analytical challenges in vitamin K analysis, as it is the most lipophilic and least abundant of the fat-soluble vitamins. In this study, a simple and fast plasma sample preparation procedure was developed using phospholipid removal in combination with a chromatographic analysis using a Raptor Biphenyl column. The method provides a novel solution towards vitamin K research and high-throughput clinical diagnosis.

Bile Acid Profiling and Quantification in Human Plasma using LC-MS/MS
Dan Li, Frances Carroll, Ravali Alagandula, Shun-Hsin Liang, Justin Steimling, Landon Wiest, Susan Steinike, Paul Connolly, Ty Kahler, David Bell (presenter)
Restek Corporation
For more information, email David Bell.
Download a PDF of the full presentation.
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Bile acids are a group of major catabolic products of cholesterol. They are important biomarkers for signaling potential harmful side effects for new drug development. Quantitation of bile acids in matrices proves to be very challenging due to a number of factors, including the similarity of structures, varying polarities and stereochemistries, limited fragmentation for unconjugated bile acids in mass spectrometer, high endogenous levels, and matrix effects caused by phospholipids or triglycerides. In this study, a rapid, robust, selective, and reliable LC-MS/MS method was established and validated in human plasma using a Raptor C18 column with baseline separation of 17 bile acids in 6 minutes.

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Restek at MSACL EU 2018

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