Analysis by LC-MS/MS of polar pesticides in fruits and vegetables using new hybrid stationary phase

Author(s): Víctor Cutillas, Amadeo R. Fernández-Alba

University of Almería, Department of Chemistry and Physics, Agrifood Campus of International Excellence (ceiA3), Ctra. Sacramento s/n, La Cañada de San Urbano, Almería 04120, Spain

Published By: MethodsX

Issue: Volume 8

Year of Publication: 2021

Link: https://doi.org/10.1016/j.mex.2021.101306

Abstract: Highly polar pesticides are frequently used in agriculture. However, their physicochemical properties make very difficult the analysis of these compounds following common procedures. Polar pesticides show poor retention and peak shapes in the common stationary phases used for multiresidue methods of pesticides. For this reason, multiple columns with different stationary phases have been developed to perform the analysis of these particular compounds. The column evaluated in this method uses a new hybrid stationary phase with a mixed-mode between hydrophilic interaction liquid chromatography (HILIC) and ion exchange interactions. The retention modes are dependant on mobile phase conditions and can be easily switched. The aim of this study can be summarized in the next bullet points:

• Performance evaluation of 10 anionic compounds in different column sizes of the hybrid stationary phase.
• Validation of the method in terms of sensitivity, linearity, and matrix effects in four different matrices: tomato, orange, onion and quince.
• Improvement of the retention time robustness.

Note(s): This study was done using Raptor Polar X columns.