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High-Throughput LC-MS/MS Analysis of Phosphatidylethanol in Whole Blood

Featured Application: Phosphatidylethanol (PEth) in Blood on Raptor FluoroPhenyl

  • Simple protein precipitation sample prep streamlines workflow.
  • Fast 3.5-minute gradient cycle time supports faster sample throughput.
  • Easy-to-prepare mobile phases are quick to make consistently.

Phosphatidylethanol (PEth) is a group of phospholipids formed through an enzymatic reaction between ethanol and phosphatidylcholine on cell membranes. Among the multiple homologues of PEth, PEth-16:0/18:1 is the predominant molecule extracted from human erythrocytes. Analysis of phosphatidylethanol 16:0/18.1 is an important biomarker of alcohol consumption, and it can be measured in whole blood with a detection window of up to 3-4 weeks. Previously, PEth was considered a biomarker for high and sustained alcohol consumption, but with the application of highly sensitive LC-MS/MS techniques, it is now possible to use PEth concentration in blood to differentiate chronic drinking from social drinking or as a marker of absolute abstinence.

The LC-MS/MS method shown here was developed using a Raptor FluoroPhenyl column because of the column’s unique selectivity and retention mechanisms. Specific and sensitive analysis of phosphatidylethanol 16:0/18:1 in whole blood was achieved using a simple protein precipitation sample preparation procedure and a fast 3.5-minute LC cycle time. The high-quality chromatographic results that were achieved in a short analysis time make this approach ideal for labs needing a low-cost, high-throughput PEth method for monitoring alcohol consumption.