• 40を超える異性体、10の薬物クラス、ESIネガティブの22の化合物を含む231化合物を1本のカラムで10分で分析します。
• 実績のあるRestek Biphenylの選択性を備えたRaptor SPP LCカラムには、汎用性とマルチクラス対応性があります。
• ピークはテーリングすることなく、重要な異性体の優れた分離を達成します。
• シンプルな移動相を用いて感度良く、高速で信頼性の高いハイスループットLC-MS/MS分析をおこないます。

疼痛マネジメント用の薬の使用は着実に増加しています。その結果、病院やリファレンスラボでは、薬物乱用を予防し、患者の安全性と服薬レジメンの遵守を確保するために、多種多様な疼痛マネジメントに使用される薬のスクリーニングを必要とする患者のサンプルが増えています。治療薬モニタリングは、カットオフ値が低く、潜在的なマトリックス干渉、および異性体薬物化合物のために困難です。これらの課題に対処すべく、多くの薬物試験施設は、その速度、感度、および特異性を増すために、質量分析(LC-MS/MS)と組み合わせた液体クロマトグラフィーに変更しています。

以下の分析に示すように、RestekのRaptor Biphenylカラムは、成功をもたらすLC-MS/MS疼痛薬スクリーニング法の開発に理想的です。 非常に高い保持力と独特な選択性により、40種類以上の異性体を含む231種類の多クラス薬物化合物と代謝産物をわずか10分で分析することができます。さらに、オピオイド、抗不安薬、バルビツール酸、NSAIDおよび鎮痛薬、抗うつ薬、抗てんかん薬、抗精神病薬、幻覚薬、および刺激薬に対してそれぞれ専用のパネルがRaptor Biphenylカラムで最適化され、確認および定量分析に使用されています。

	Peaks	tR (min)	Conc. (ng/mL)	Precursor Ion	Product Ion	Product Ion
1.	trans-3-Hydroxycotinine	1.32	25	193.1	80.0	134.0
2.	Norcotinine	1.33	25	163.1	80.1	118.1
3.	Nicotine	1.40	25	163.2	130.1	117.1
4.	BZP	1.47	25	177.2	91.1	65.1
5.	Anabasine	1.53	25	163.2	120.1	80.1
6.	Noroxymorphone	1.78	25	288.1	270.1	212.9
7.	Lisdexamfetamine	1.79	25	264.2	84.1	91.0
8.	Phenelzine sulfate	1.90	25	137.2	105.1	77.1
9.	Acetaminophen	1.93	100	152.2	93.0	65.0
10.	Pregabalin	2.21	25	160.2	142.1	55.0
11.	Cotinine	2.27	25	177.2	80.0	98.1
12.	Morphine	2.27	25	286.2	152.1	165.0
13.	(+)-Ephedrine	2.37	25	166.2	148.1	115.0
14.	Oxymorphone	2.38	25	302.1	227.2	198.2
15.	Hydromorphone	2.61	25	286.2	184.9	156.9
16.	Amphetamine	2.63	25	136.2	91.0	65.1
17.	DMAA	2.65	25	116.1	57.1	99.2
18.	Gabapentin	2.71	25	172.2	154.0	137.1
19.	Methylephedrine	2.83	25	180.2	162.0	115.1
20.	Levetiracetam	2.84	25	171.1	125.9	69.2
21.	Desmethylolanzapine	2.94	25	299.1	256.1	198.0
22.	Norcodeine	2.96	25	286.1	151.9	115.0
23.	Olanzapine	3.12	25	313.2	256.1	198.1
24.	Methamphetamine	3.13	25	150.3	91.1	119.0
25.	MDA	3.18	25	180.1	163.1	105.2
26.	(-)-Naloxone	3.27	25	328.3	310.1	212.3
27.	Phentermine	3.29	25	150.2	91.1	133.1
28.	Noroxycodone	3.32	25	302.1	227.0	197.9
29.	Methylone	3.36	25	208.1	160.1	132.1
30.	O-Desmethyl-cis-tramadol	3.37	25	250.1	58.0	42.0
31.	(-)-Dihydrocodeine	3.38	25	302.2	199.0	128.2
32.	Norhydrocodone	3.43	25	286.1	199.0	128.2
33.	Codeine	3.43	25	300.2	152.0	165.1
34.	6-Acetylmorphine	3.47	25	328.2	165.0	211.0
35.	MDMA	3.60	25	194.2	163.0	135.1
36.	Desomorphine	3.61	25	272.1	152.1	165.0
37.	Oxycodone	3.64	25	316.2	298.0	169.0
38.	Naltrexone	3.71	25	342.2	324.0	267.0
39.	Levamisole	3.75	25	205.1	178.0	91.1
40.	Hydrocodone	3.76	25	300.1	199.0	128.0
41.	Diethylpropion	3.80	25	206.2	105.1	100.1
42.	Mephedrone	3.80	25	178.2	145.0	160.1
43.	2-Oxo-3-hydroxy-LSD	3.82	25	356.3	237.0	222.0
44.	o-Desmethylvenlafaxine	3.84	25	264.1	58.1	107.0
45.	6-β-Naltrexol	3.91	25	344.3	326.1	308.1
46.	Tapentadol-β-D-glucuronide	3.91	25	398.3	222.1	107.0
47.	7-Aminonitrazepam	3.95	25	252.2	121.1	94.2
48.	MDEA	4.00	25	208.1	163.0	105.2
49.	m-Hydroxybenzoylecgonine	4.02	25	306.1	168.1	121.0
50.	Lamotrigine	4.09	25	256.0	211.1	145.0
51.	Ritalinic acid	4.11	25	220.1	84.1	56.2
52.	N-Desmethyltapentadol	4.16	25	208.1	121.2	107.1
53.	Norbuprenorphine glucuronide	4.18	25	590.5	414.3	165.0
54.	Zonisamide	4.23	25	212.9	132.1	77.1
55.	mCPP	4.26	25	197.1	154.0	118.1
56.	Norketamine	4.28	25	224.1	125.0	89.1
57.	Nalbuphine	4.33	25	358.2	340.1	115.0
58.	Norfentanyl	4.33	25	233.1	84.1	55.0
59.	(+/-)-Hydroxybupropion	4.35	25	256.0	130.2	166.0
60.	Tapentadol	4.37	25	222.1	107.1	121.0
61.	Lacosamide	4.47	25	251.5	91.1	108.1
62.	Dextrorphan	4.50	25	258.2	157.0	201.2
63.	Levorphanol	4.50	25	258.2	156.8	199.2
64.	7-Hydroxyquetiapine	4.51	25	400.3	269.0	208.0
65.	Molindone	4.51	25	277.2	100.1	70.2
66.	Tramadol	4.52	25	264.2	58.0	77.1
67.	Zolpidem phenyl-4-carboxylic acid	4.60	25	338.2	265.1	219.0
68.	Felbamate	4.62	25	239.1	117.1	177.8
69.	N-Desmethyl-cis-tramadol	4.65	25	250.2	44.0	232.1
70.	Ketamine	4.66	25	238.1	125.1	89.1
71.	Benzoylecgonine	4.67	25	290.1	168.1	77.1
72.	Methylphenidate	4.68	25	234.1	84.1	56.1
73.	Normeperidine	4.76	25	234.1	160.2	91.0
74.	Meprobamate	4.79	25	219.1	158.2	97.0
75.	Meperidine	4.80	25	248.1	220.1	174.1
76.	Bupropion	4.81	25	240.0	184.1	130.2
77.	Heroin	4.85	25	370.4	328.2	164.9
78.	Cocaine	5.01	25	304.2	182.0	77.1
79.	7-Aminoclonazepam	5.02	25	286.1	121.2	250.1
80.	Zopiclone	5.04	25	389.2	245.0	217.2
81.	N-Desmethylmirtazapine	5.10	25	252.1	195.1	209.2
82.	Norcocaine	5.11	25	290.1	168.0	136.1
83.	Phenobarbital*	5.11	500	230.9	187.8	85.0
84.	Buprenorphine glucuronide	5.12	25	644.3	468.2	55.1
85.	MDPV	5.15	25	276.2	135.1	126.1
86.	Venlafaxine	5.15	25	278.4	260.4	121.2
87.	Milnacipran	5.24	25	247.2	100.1	129.1
88.	Pentazocine	5.29	25	286.2	218.1	69.2
89.	Mirtazapine	5.31	25	266.1	195.1	72.1
90.	Butalbital*	5.31	500	223.0	180.0	84.9
91.	Norbuprenorphine	5.35	50	414.3	152.2	165.2
92.	LSD	5.39	25	324.3	223.1	208.0
93.	Butorphanol	5.43	25	328.3	310.3	131.2
94.	Fosphenytoin	5.43	25	363.1	265.9	207.8
95.	Topiramate	5.44	25	340.2	264.2	223.9
96.	Cocaethylene	5.48	25	318.2	196.1	82.0
97.	Clozapine	5.48	25	328.2	271.1	193.1
98.	9-Hydroxyrisperidone	5.51	25	427.3	207.1	110.2
99.	Acetyl fentanyl	5.58	25	323.3	188.0	105.0
100.	Didesmethyl citalopram HCl	5.73	25	297.1	261.9	109.1
101.	7-Aminoflunitrazepam	5.75	25	284.1	135.0	227.1
102.	Amobarbital*	5.75	500	225.0	182.0	84.8
103.	Zolpidem	5.76	25	308.2	235.2	218.9
104.	N-Desmethylcitalopram	5.77	25	311.1	262.1	109.1
105.	Isocarboxazid	5.77	25	232.0	91.1	65.2
106.	Pentobarbital*	5.79	500	225.0	182.0	84.9
107.	Escitalopram	5.80	25	325.3	109.1	261.9
108.	Citalopram	5.80	25	325.2	109.1	262.0
109.	Diphenhydramine	5.80	25	256.1	167.0	152.1
110.	Desmethyldoxepin	5.88	25	266.1	107.1	115.0
111.	Ezogabine	5.91	25	304.0	230.1	109.1
112.	Doxepin	5.96	25	280.1	107.2	77.1
113.	Haloperidol	5.96	25	377.2	123.0	95.1
114.	Fluvoxamine	5.97	25	319.3	71.1	257.8
115.	Trazodone	5.99	25	372.3	176.1	148.0
116.	Norfluoxetine	6.01	25	296.3	134.3	104.9
117.	Fentanyl	6.02	25	337.3	188.0	105.1
118.	Oxcarbazepine	6.02	25	253.1	180.0	208.1
119.	Risperidone	6.03	25	411.2	191.0	110.1
120.	Buprenorphine	6.04	50	468.3	55.1	414.2
121.	Fluoxetine	6.05	25	310.1	148.0	115.1
122.	Secobarbital*	6.08	500	237.0	193.9	84.8
123.	Phenytoin	6.12	25	253.0	103.9	181.7
124.	Carisoprodol	6.13	25	261.1	176.0	62.0
125.	Flurazepam	6.14	25	388.2	315.2	183.0
126.	Chlordiazepoxide	6.14	25	300.1	282.0	227.0
127.	Dextromethorphan	6.16	25	272.2	215.1	170.9
128.	PCP	6.18	25	244.2	86.1	159.1
129.	Tenoxicam	6.18	25	338.1	121.0	95.0
130.	Buspirone	6.20	25	386.3	122.1	95.0
131.	Quetiapine	6.28	25	384.2	253.0	221.2
132.	Norpropoxyphene	6.29	25	326.4	252.0	91.1
133.	Propoxyphene	6.30	25	340.3	265.9	58.1
134.	Asenapine	6.35	25	286.2	165.1	229.1
135.	Ziprasidone	6.40	25	413.2	194.1	130.0
136.	Protriptyline	6.42	25	264.1	191.1	165.2
137.	Promazine	6.42	25	285.1	58.0	86.0
138.	Midazolam	6.43	25	326.2	291.1	248.9
139.	Desipramine	6.43	25	267.1	72.1	193.1
140.	Amoxapine	6.44	25	314.2	271.0	193.1
141.	Sufentanil	6.48	25	387.2	238.1	111.1
142.	Imipramine	6.51	25	281.1	86.2	58.1
143.	Paroxetine	6.51	25	330.2	192.2	70.1
144.	Loxapine	6.52	25	328.1	271.1	193.0
145.	Maprotiline	6.52	25	278.1	250.2	191.0
146.	Duloxetine	6.52	25	298.1	154.1	188.2
147.	Metaxalone	6.52	25	222.1	161.1	77.1
148.	Carbamazepine	6.55	10	237.1	194.1	165.0
149.	Cyclobenzaprine	6.56	25	276.2	215.0	189.0
150.	EDDP	6.57	25	278.2	234.3	249.2
151.	Nortriptyline	6.58	25	264.1	91.1	115.2
152.	Mitragynine	6.59	25	399.3	174.2	159.0
153.	Eslicarbazepine	6.60	25	297.2	194.0	237.1
154.	Amitriptyline	6.65	25	278.1	91.1	202.1
155.	α-Hydroxymidazolam	6.70	25	342.2	324.1	202.9
156.	Trimipramine	6.75	25	295.2	100.2	58.2
157.	N-Desmethylflunitrazepam	6.80	25	300.1	254.1	198.1
158.	Lorazepam	6.82	25	321.1	275.0	229.0
159.	Mesoridazine	6.83	25	387.2	126.1	98.2
160.	Tiagabine	6.89	25	376.2	246.8	111.1
161.	Ketorolac	6.90	25	256.1	104.9	77.0
162.	Chlorpromazine	6.91	25	320.1	58.1	86.1
163.	Loxoprofen*	6.93	200	245.0	83.2	80.9
164.	Clomipramine	6.95	25	315.3	86.0	58.0
165.	Oxazepam	6.96	25	287.1	241.2	268.8
166.	Methadone	6.98	25	310.2	264.9	105.1
167.	Sertraline	6.98	25	306.2	158.9	275.1
168.	Dehydro aripiprazole	7.01	25	446.2	285.0	98.1
169.	Clonazepam	7.01	25	316.1	270.0	214.1
170.	Nitrazepam	7.01	25	282.1	235.9	180.1
171.	Lornoxicam	7.02	100	373.1	121.1	95.0
172.	25I-NBOMe	7.05	25	428.1	121.2	91.1
173.	Salsalate*	7.05	100	256.8	136.8	92.9
174.	Desalkylflurazepam	7.09	25	289.0	140.1	104.0
175.	Tolmetin	7.10	25	258.1	119.1	91.0
176.	α-Hydroxytriazolam	7.12	25	359.1	330.9	175.9
177.	Fluphenazine	7.16	25	438.3	171.1	143.2
178.	2-Hydroxyethylflurazepam	7.16	25	333.1	211.1	109.0
179.	Ketoprofen*	7.17	100	253.0	209.0	-
180.	Naproxen*	7.17	200	229.0	168.9	184.9
181.	EMDP	7.19	25	264.2	235.2	220.3
182.	Aripiprazole	7.20	25	448.2	285.0	176.1
183.	Perphenazine	7.22	25	404.2	171.1	143.2
184.	Etoricoxib	7.24	25	359.2	280.0	279.2
185.	Methaqualone	7.24	25	251.1	91.1	132.2
186.	α-Hydroxyalprazolam	7.25	25	325.1	297.0	216.2
187.	Trifluoperazine	7.28	25	408.2	141.2	113.1
188.	Piroxicam	7.34	25	332.1	95.2	78.1
189.	Ibuprofen*	7.34	200	205.0	160.9	-
190.	Prochlorperazine	7.36	25	374.1	141.0	113.1
191.	Etodolac*	7.36	50	286.0	241.9	212.0
192.	Nordiazepam	7.37	25	271.0	139.9	208.0
193.	Phenazepam	7.39	25	349.1	206.2	179.0
194.	Fenoprofen*	7.40	200	240.9	92.8	-
195.	Zaleplon	7.43	25	306.1	264.0	236.0
196.	Clobazam	7.43	25	301.0	259.1	224.0
197.	Flurbiprofen*	7.48	200	243.0	199.0	196.8
198.	Thiothixene	7.50	25	444.2	221.2	235.0
199.	Flunitrazepam	7.55	25	314.2	267.9	239.1
200.	Meloxicam	7.55	25	352.1	115.0	141.0
201.	Triazolam	7.58	25	343.1	307.9	315.0
202.	Estazolam	7.58	25	295.1	266.9	205.1
203.	Celecoxib	7.60	25	382.2	362.0	300.1
204.	Parecoxib	7.60	25	371.2	234.0	165.1
205.	Temazepam	7.61	25	301.1	255.1	282.9
206.	Sulindac	7.70	25	357.2	233.1	248.0
207.	Alprazolam	7.72	25	309.1	280.9	204.9
208.	Flufenamic acid*	7.75	50	279.9	236.1	176.2
209.	Thioridazine	7.76	25	371.2	126.1	98.1
210.	Diclofenac*	7.78	100	293.9	249.8	-
211.	Nabumetone	7.80	25	229.1	171.1	128.1
212.	Oxaprozin	7.87	25	294.1	103.0	234.2
213.	HU-211	8.03	25	387.3	155.9	128.1
214.	JWH‐200	8.04	25	385.3	155.0	127.1
215.	Diazepam	8.04	25	285.1	192.9	153.9
216.	Mefenamic acid	8.04	25	242.1	224.1	-
217.	Tolfenamic acid*	8.17	50	259.9	215.8	-
218.	Meclofenamic acid*	8.18	200	293.9	257.8	177.7
219.	(+/-)-CP 47,497*	8.27	200	317.1	245.1	159.0
220.	Indomethacin*	8.29	50	356.0	311.8	296.9
221.	Perampanel	8.32	25	350.2	219.1	247.1
222.	(+/-)-CP 47,497 C8 homologue*	8.42	200	331.1	259.0	159.1
223.	11-Nor-9-carboxy-delta-9-THC*	8.44	25	343.0	298.9	244.8
224.	Prazepam	8.51	25	325.2	271.0	139.9
225.	XLR-11	8.60	25	330.3	125.2	144.0
226.	Δ9-THC	8.76	25	315.2	193.0	123.2
227.	UR-144	8.78	25	312.3	125.1	214.1
228.	JWH-250	8.96	25	336.3	120.9	91.1
229.	THCA-A*	9.12	25	357.1	312.9	245.0
230.	JWH-073	9.21	25	328.2	155.0	127.1
231.	JWH‐018	9.35	25	342.3	127.1	155.1

*Analyzed using negative polarity.

Column	Raptor Biphenyl (cat.# 9309A12)
Dimensions:	100 mm x 2.1 mm ID
Particle Size:	2.7 µm
Guard Column:	Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252)
Temp.:	30 °C
Standard/Sample
Diluent:	Water
Inj. Vol.:	5 µL
Mobile Phase
A:	Water + 0.1% formic acid + 2 mM ammonium formate
B:	Methanol + 0.1% formic acid + 2 mM ammonium formate
	Time (min) Flow (mL/min) %A %B 0.00 0.6 95 5 9.00 0.6 0 100 10.00 0.6 0 100 10.01 0.6 95 5 12.00 0.6 95 5

Detector	MS/MS
Ion Mode:	ESI+/ESI-
Mode:	Scheduled MRM
Instrument	UHPLC
Notes	1. Positive and negative polarity data were collected simultaneously from a single injection. 2. Ibuprofen, fosphenytoin, and topiramate degrade quickly in solution. 3. Levorphanol and dextrorphan are enantiomers and are not resolved. 4. Escitalopram is a pure enantiomer and citalopram is a racemic mixture of both enantiomers. These compounds are not resolved. 5. DMAA consists of 4 stereoisomers, 2 of which are partially resolved. 6. Doxepin and desmethylydoxepin consist of a mixture of cis/trans isomers. The isomers are fully resolved and the retention time identifies the main isomer component. 7. Lornoxicam, meloxicam, etodolac, oxaprozin, indomethacin, and mefenamic acid can be detected by positive or negative polarity. The polarity chosen in this chromatogram provides optimal response.

Download PDF