PFAS Analysis by CDC 6304.09 for Serum
- Sample Matrix: serum
- Sample Preparation: online solid-phase extraction
- Detection Technique: LC-MS/MS
Online Solid Phase Extraction-High Performance Liquid Chromatography-Turbo Ion Spray-Tandem Mass Spectrometry (online SPE-HPLC-TISMS/MS)
Method CDC 6304.09 is used for the analysis of 15 PFAS in serum. It combines online solid-phase extraction sample preparation with turbo ion spray LC-MS/MS analysis. Notably, PFOS is analyzed separately as n-perfluorooctane sulfonate (n-PFOS) and the sum of perfluoromethylheptane sulfonate isomers (sm-PFOS). Similarly, PFOA is analyzed as separately as n-perfluorooctanoate (n-PFOA) and the sum of branched perfluorooctanoate isomers (sb-PFOA). The full analyte list and recommended products for PFAS analysis serum by CDC 6304.09 are provided below. In addition, you can register and directly compare analyte lists and matrices across PFAS analysis methods here or visit www.restek.com/PFAS to explore our technical resources for PFAS analysis.
Analyte List for CDC 6304.09 for PFAS Analysis
PFAS acronyms, compound names, and CAS numbers vary across methods and guidelines, and they also depend on compound form (e.g., neutral vs. salt). The following list uses standardized terminology to allow easy comparison across the methods and guidelines in our comprehensive guide. Please refer to CDC 6304.09 for method-specific nomenclature.
| Acronym | Compound Name | CAS# |
| NEtFOSAA | N-ethyl perfluorooctanesulfonamidoacetic acid | 2991-50-6 |
| NMeFOSAA | N-methyl perfluorooctanesulfonamidoacetic acid | 2355-31-9 |
| PFDA | Perfluorodecanoic acid | 335-76-2 |
| PFHpA | Perfluoroheptanoic acid | 375-85-9 |
| PFHxS | Perfluoro-1-hexanesulfonic acid | 355-46-4 |
| PFHxA | Perfluorohexanoic acid | 307-24-4 |
| PFNA | Perfluorononanoic acid | 375-95-1 |
| PFOS | Perfluorooctanesulfonic acid | 1763-23-1 |
| PFOA | Perfluorooctanoic acid | 335-67-1 |
| PFUnA | Perfluoroundecanoic acid | 2058-94-8 |
| HFPO-DA, GenX | Hexafluoropropylene oxide dimer acid | 13252-13-6 |
| PFHpS | Perfluoro-1-heptanesulfonic acid | 375-92-8 |
| FOSA | Perfluoro-1-octanesulfonamide | 754-91-6 |
| 9Cl-PF3ONS | 9-Chlorohexadecafluoro-3-oxanonane-1-sulfonic acid (F-53B Major) | 756426-58-1 |
| ADONA | 4,8-Dioxa-3H-perfluorononanoic acid | 919005-14-4 |
Recommended Products for CDC 6304.09 for PFAS Analysis
ANALYSIS
Analytical LC Columns:
- Raptor C18 SPP column: 2.7 μm, 50 x 2.1 mm (cat.# 9304A52)
- Force C18 FPP column: 1.8 μm, 50 x 2.1 mm (cat.# 9634252)
Delay Columns:
- PFAS delay column: 5 µm, 50 x 2.1 mm (cat.# 27854)
- PFAS delay column: 5 µm, 50 x 3.0 mm (cat.# 580959 [custom product])
Guard Columns:
- UltraShield UHPLC precolumn filter: 0.2 µm frit (cat.# 25809)
- EXP direct connect holder for EXP guard cartridges (cat.# 25808)
- Raptor C18 EXP guard column cartridge: 2.7 µm, 5 x 2.1 mm (cat.# 9304A0252)
SAMPLE HANDLING
Sample Prep:
Note that this CDC method is based on automated online SPE using C8 or C18 SPE materials. The product listed here is recommended for off-line SPE.
- Resprep S-DVB SPE cartridge: 6 mL/500 mg (cat.# 28937)
Vacuum Manifolds:
Vacuum Pumps:
Note that this CDC method is based on automated online SPE using C8 or C18 SPE materials. The products listed here are recommended for off-line SPE.
- Chemker 300 PTFE vacuum pump: 20 L/min, AC110 V, 60 Hz (cat.# 27426)
- Chemker 300 PTFE vacuum pump: 18 L/min, AC220 V, 50 Hz (cat.# 27427)
GENERAL LAB SUPPLIES
- Polypropylene vials: 100-pk. (cat.# 23243)
- Polypropylene vials: 1000-pk. (cat.# 23246)
- Polyethylene vial caps: 100-pk. (cat.# 23244)
- Polyethylene vial caps: 1000-pk. (cat.# 23247)
- Centrifuge tubes: 50 mL, 50-pk. (cat.# 25846)
- Centrifuge tubes: 50 mL, 500-pk. (cat.# 28290)
- Norm-Ject plastic syringe: 10 mL Luer Lock tip (cat.# 22775)
- Norm-Ject plastic syringe: 5 mL Luer Lock tip (cat.# 22774)
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