PFAS Analysis by ISO 21675:2019 for Water
- Sample Matrix: water containing <2 g/L solids
- Sample Preparation: WAX solid-phase extraction
- Detection Technique: LC-MS/MS
Determination of perfluoroalkyl and polyfluoroalkyl substances (PFAS) in water—Method using solid phase extraction and liquid chromatography-tandem mass spectrometry (LC-MS/MS)
ISO 21675:2019 is used for the analysis of 30 PFAS in water containing <2 g/L solids. It is not available from Restek but may be purchased from other sources. The full analyte list and recommended products for ISO 21675:2019 are provided below. In addition, you can register and directly compare analyte lists and matrices across PFAS analysis methods here or visit www.restek.com/PFAS to explore our technical resources for PFAS analysis.
Analyte List for ISO 21675:2019 for PFAS Analysis
PFAS acronyms, compound names, and CAS numbers vary across methods and guidelines, and they also depend on compound form (e.g., neutral vs. salt). The following list uses standardized terminology to allow easy comparison across the methods and guidelines in our comprehensive guide. Please refer to ISO 21675:2019 for method-specific nomenclature.
| Acronym | PFAS Name | CAS |
| NEtFOSAA | N-Ethyl perfluorooctanesulfonamidoacetic acid | 2991-50-6 |
| NMeFOSAA | N-Methyl perfluorooctanesulfonamidoacetic acid | 2355-31-9 |
| PFBS | Perfluoro-1-butanesulfonic acid | 375-73-5 |
| PFDA | Perfluorodecanoic acid | 335-76-2 |
| PFDoA | Perfluorododecanoic acid | 307-55-1 |
| PFHpA | Perfluoroheptanoic acid | 375-85-9 |
| PFHxS | Perfluoro-1-hexanesulfonic acid | 355-46-4 |
| PFHxA | Perfluorohexanoic acid | 307-24-4 |
| PFNA | Perfluorononanoic acid | 375-95-1 |
| PFOS | Perfluorooctanesulfonic acid | 1763-23-1 |
| PFOA | Perfluorooctanoic acid | 335-67-1 |
| PFTeDA | Perfluorotetradecanoic acid | 376-06-7 |
| PFTrDA | Perfluorotridecanoic acid | 72629-94-8 |
| PFUnA | Perfluoroundecanoic acid | 2058-94-8 |
| HFPO-DA, GenX | Hexafluoropropylene oxide dimer acid | 13252-13-6 |
| PFBA | Perfluorobutanoic acid | 375-22-4 |
| PFPeA | Perfluoropentanoic acid | 2706-90-3 |
| PFDS | Perfluoro-1-decanesulfonic acid | 335-77-3 |
| PFHpS | Perfluoro-1-heptanesulfonic acid | 375-92-8 |
| FOSA | Perfluoro-1-octanesulfonamide | 754-91-6 |
| 6:2 FTS | 1H,1H,2H,2H-Perfluorooctane sulfonic acid | 27619-97-2 |
| 8:2 FTS | 1H,1H,2H,2H-Perfluorodecane sulfonic acid | 39108-34-4 |
| 8:2 FTUCA, FOUEA | 2H-Perfluoro-2-decenoic acid (8:2) | 70887-84-2 |
| 8:2 diPAP | 8:2 Polyfluoroalkyl phosphate diester | 678-41-1 |
| NMeFOSA | N-Methylperfluorooctanesulfonamide | 31506-32-8 |
| NEtFOSA | N-Ethylperfluorooctanesulfonamide | 4151-50-2 |
| PFHxDA | Perfluoro-n-hexadecanoic acid | 67905-19-5 |
| PFODA | Perfluoro-n-octadecanoic acid | 16517-11-6 |
| 9Cl-PF3ONS | 9-Chlorohexadecafluoro-3-oxanonane-1-sulfonic acid (F-53B Major) | 756426-58-1 |
| ADONA | 4,8-Dioxa-3H-perfluorononanoic acid | 919005-14-4 |
Recommended Products for ISO 21675:2019 for PFAS Analysis
ANALYSIS
Analytical LC Columns:
- Raptor C18 SPP column: 50 x 2.1 mm, 2.7 μm (cat.# 9304A52)
- Force C18 FPP column: 50 x 2.1 mm, 1.8 μm (cat.# 9634252)
Delay Columns:
- PFAS delay column: 5 µm, 50 x 2.1 mm (cat.# 27854)
- PFAS delay column: 5 µm, 50 x 3.0 mm (cat.# 580959 [custom product])
SAMPLE HANDLING
Sample Prep:
- 30 mm syringe filter: 0.45 µm, nylon (cat.# 23981)*
- 30 mm syringe filter: 0.22 µm, nylon (cat.# 23980)*
- Resprep polymeric SPE cartridge: WAX, 6 mL/500 mg, 30 µm (cat.# 28291)
- Resprep polymeric SPE cartridge: WAX, 6 mL/200 mg, 30 µm (cat.# 28292)
- Polypropylene sample reservoir: 75 mL (cat.# 26015)
- Polypropylene connectors (cat.# 26007)
- Q-sep multispeed centrifuge for QuEChERS (cat.# 28295)
*Note that a glass fiber filter is mentioned in the method. However, nylon and polypropylene are widely accepted filter membrane materials for PFAS analysis.
Vacuum Manifolds:
Vacuum Pumps:
- Chemker 300 PTFE vacuum pump: 20 L/min, AC110 V, 60 Hz (cat.# 27426)
- Chemker 300 PTFE vacuum pump: 18 L/min, AC220 V, 50 Hz (cat.# 27427)
GENERAL LAB SUPPLIES
- Polypropylene vials: 100-pk. (cat.# 23243)
- Polypropylene vials: 1000-pk. (cat.# 23246)
- Polyethylene vial caps: 100-pk. (cat.# 23244)
- Polyethylene vial caps: 1000-pk. (cat.# 23247)
- Centrifuge tubes: 50 mL, 50-pk. (cat.# 25846)
- Centrifuge tubes: 50 mL, 500-pk. (cat.# 28290)
- Norm-Ject plastic syringe: 10 mL Luer Lock tip (cat.# 22775)
- Norm-Ject plastic syringe: 5 mL Luer Lock tip (cat.# 22774)
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