PFAS Analysis by U.S. EPA Method 533 for Drinking Water
- Sample Matrix: drinking water
- Sample Preparation: isotope dilution anion exchange solid-phase extraction
- Detection Technique: LC-MS/MS
Determination of Per- and Polyfluorinated Alkyl Substances in Drinking Water by Isotope Dilution Anion Exchange Solid Phase Extraction and Liquid Chromatography/Tandem Mass Spectrometry
U.S. EPA Method 533 is used for the analysis of 25 PFAS in drinking water. It combines solid-phase extraction sample preparation using weak anion exchange (WAX) cartridges with LC-MS/MS analysis on a C18 column. The method allows flexibility in some aspects, such as sample volume, LC column, LC conditions, and detector conditions, but changes to sample preservation, quality control requirements, and the extraction procedure are prohibited. In addition, the ratio of sorbent mass to aqueous sample volume cannot be reduced. The full analyte list and recommended products for PFAS analysis in drinking water by Method 533 are provided below. In addition, you can register and directly compare analyte lists and matrices across PFAS analysis methods here or visit www.restek.com/PFAS to explore our technical resources for PFAS analysis.
Analyte List for U.S. EPA Method 533 for PFAS Analysis
PFAS acronyms, compound names, and CAS numbers vary across methods and guidelines, and they also depend on compound form (e.g., neutral vs. salt). The following list uses standardized terminology to allow easy comparison across the methods and guidelines in our comprehensive guide. Please refer to U.S. EPA Method 533 for method-specific nomenclature.
| Acronym | Compound Name | CAS# |
| PFBS | Perfluoro-1-butanesulfonic acid | 375-73-5 |
| PFDA | Perfluorodecanoic acid | 335-76-2 |
| PFDoA | Perfluorododecanoic acid | 307-55-1 |
| PFHpA | Perfluoroheptanoic acid | 375-85-9 |
| PFHxS | Perfluoro-1-hexanesulfonic acid | 355-46-4 |
| PFHxA | Perfluorohexanoic acid | 307-24-4 |
| PFNA | Perfluorononanoic acid | 375-95-1 |
| PFOS | Perfluorooctanesulfonic acid | 1763-23-1 |
| PFOA | Perfluorooctanoic acid | 335-67-1 |
| PFUnA | Perfluoroundecanoic acid | 2058-94-8 |
| HFPO-DA, GenX | Hexafluoropropylene oxide dimer acid | 13252-13-6 |
| PFBA | Perfluorobutanoic acid | 375-22-4 |
| PFPeA | Perfluoropentanoic acid | 2706-90-3 |
| PFHpS | Perfluoro-1-heptanesulfonic acid | 375-92-8 |
| PFPeS | Perfluoro-1-pentanesulfonic acid | 2706-91-4 |
| 4:2 FTS | 1H,1H,2H,2H-Perfluorohexane sulfonic acid | 757124-72-4 |
| 6:2 FTS | 1H,1H,2H,2H-Perfluorooctane sulfonic acid | 27619-97-2 |
| 8:2 FTS | 1H,1H,2H,2H-Perfluorodecane sulfonic acid | 39108-34-4 |
| 9Cl-PF3ONS | 9-Chlorohexadecafluoro-3-oxanonane-1-sulfonic acid (F-53B Major) | 756426-58-1 |
| 11Cl-PF3OUdS | 11-Chloroeicosafluoro-3-oxaundecane-1-sulfonic acid (F-53B Minor) | 763051-92-9 |
| ADONA | 4,8-Dioxa-3H-perfluorononanoic acid | 919005-14-4 |
| PFEESA | Perfluoro(2-ethoxyethane)sulfonic acid | 113507-82-7 |
| NFDHA | Nonafluoro-3,6-dioxaheptanoic acid | 151772-58-6 |
| PFMPA | Perfluoro-3-methoxypropanoic acid | 377-73-1 |
| PFMBA | Perfluoro-4-methoxybutanoic acid | 863090-89-5 |
Recommended Products for U.S. EPA Method 533 for PFAS Analysis
ANALYSIS
Analytical LC Columns:
- Raptor C18 SPP column: 2.7 μm, 50 x 2.1 mm (cat.# 9304A52)
- Force C18 FPP column: 1.8 μm, 50 x 2.1 mm (cat.# 9634252)
Delay Columns:
- PFAS delay column: 5 µm, 50 x 2.1 mm (cat.# 27854)
- PFAS delay column: 5 µm, 50 x 3.0 mm (cat.# 580959 [custom product])
Guard Columns:
- UltraShield UHPLC precolumn filter: 0.2 µm frit (cat.# 25809)
- EXP direct connect holder for EXP guard cartridges (cat.# 25808)
- Raptor C18 EXP guard column cartridge: 2.7 µm, 5 x 2.1 mm (cat.# 9304A0252)
STANDARDS
- PFAS 24 calibration standard: 1 µg/mL, methanol (1 mM KOH) (cat.# 30733)
- PFAS 28 calibration standard: 1 µg/mL, methanol (1 mM KOH) (cat.# 30734)
- EPA 533 PFAS calibration standard: 2 µg/mL, methanol (1 mM KOH) (cat.# 30736)
SAMPLE HANDLING
Sample Prep:
- Resprep polymeric SPE cartridge: WAX, 6 mL/500 mg, 30 µm (cat.# 28268)
- Resprep polymeric SPE cartridge: WAX, 6 mL/200 mg, 30 µm (cat.# 28292)
Sample Reservoirs:
Vacuum Manifolds:
Vacuum Pumps:
- Chemker 300 PTFE vacuum pump: 20 L/min, AC110 V, 60 Hz (cat.# 27426)
- Chemker 300 PTFE vacuum pump: 18 L/min, AC220 V, 50 Hz (cat.# 27427)
GENERAL LAB SUPPLIES
- Polypropylene vials: 100-pk. (cat.# 23243)
- Polypropylene vials: 1000-pk. (cat.# 23246)
- Polyethylene vial caps: 100-pk. (cat.# 23244)
- Polyethylene vial caps: 1000-pk. (cat.# 23247)
- Centrifuge tubes: 50 mL, 50-pk. (cat.# 25846)
- Centrifuge tubes: 50 mL, 500-pk. (cat.# 28290)
- Norm-Ject plastic syringe: 10 mL Luer Lock tip (cat.# 22775)
- Norm-Ject plastic syringe: 5 mL Luer Lock tip (cat.# 22774)
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