PFAS Analysis by U.S. EPA Method 537.1 for Drinking Water
- Sample Matrix: drinking water
- Sample Preparation: S-DVB solid-phase extraction
- Detection Technique: LC-MS/MS
Determination of Selected Per- and Polyfluorinated Alkyl Substances in Drinking Water by Solid Phase Extraction and Liquid Chromatography/Tandem Mass Spectrometry (LC-MS/MS)
U.S. EPA Method 537.1 is used for the analysis of 18 PFAS in drinking water. It combines solid-phase extraction sample preparation using styrene-divinylbenzene cartridges with LC-MS/MS analysis on a C18 column. The method allows flexibility in some aspects, such as evaporation technique, separation technique, mobile phase composition, LC column and conditions, and detector conditions, but changes to sample collection, preservation, and extraction and quality control requirements are prohibited. The full analyte list and recommended products for PFAS analysis in drinking water by Method 537.1 are provided below. In addition, you can register and directly compare analyte lists and matrices across PFAS analysis methods here or visit www.restek.com/PFAS to explore our technical resources for PFAS analysis.
Analyte List for U.S. EPA Method 537.1 for PFAS Analysis
PFAS acronyms, compound names, and CAS numbers vary across methods and guidelines, and they also depend on compound form (e.g., neutral vs. salt). The following list uses standardized terminology to allow easy comparison across the methods and guidelines in our comprehensive guide. Please refer to U.S. EPA Method 537.1 for method-specific nomenclature.
| Acronym | Compound Name | CAS# |
| NEtFOSAA | N-ethyl perfluorooctanesulfonamidoacetic acid | 2991-50-6 |
| NMeFOSAA | N-methyl perfluorooctanesulfonamidoacetic acid | 2355-31-9 |
| PFBS | Perfluoro-1-butanesulfonic acid | 375-73-5 |
| PFDA | Perfluorodecanoic acid | 335-76-2 |
| PFDoA | Perfluorododecanoic acid | 307-55-1 |
| PFHpA | Perfluoroheptanoic acid | 375-85-9 |
| PFHxS | Perfluoro-1-hexanesulfonic acid | 355-46-4 |
| PFHxA | Perfluorohexanoic acid | 307-24-4 |
| PFNA | Perfluorononanoic acid | 375-95-1 |
| PFOS | Perfluorooctanesulfonic acid | 1763-23-1 |
| PFOA | Perfluorooctanoic acid | 335-67-1 |
| PFTeDA | Perfluorotetradecanoic acid | 376-06-7 |
| PFTrDA | Perfluorotridecanoic acid | 72629-94-8 |
| PFUnA | Perfluoroundecanoic acid | 2058-94-8 |
| HFPO-DA, GenX | Hexafluoropropylene oxide dimer acid | 13252-13-6 |
| 9Cl-PF3ONS | 9-Chlorohexadecafluoro-3-oxanonane-1-sulfonic acid (F-53B Major) | 756426-58-1 |
| 11Cl-PF3OUdS | 11-Chloroeicosafluoro-3-oxaundecane-1-sulfonic acid (F-53B Minor) | 763051-92-9 |
| ADONA | 4,8-Dioxa-3H-perfluorononanoic acid | 919005-14-4 |
Recommended Products for U.S. EPA Method 537.1 for PFAS Analysis
ANALYSIS
Analytical LC Columns:
- Raptor C18 SPP column: 50 x 2.1 mm, 2.7 μm (cat.# 9304A52)
- Force C18 FPP column: 50 x 2.1 mm, 1.8 μm (cat.# 9634252)
Delay Columns:
- PFAS delay column: 5 µm, 50 x 2.1 mm (cat.# 27854)
- PFAS delay column: 5 µm, 50 x 3.0 mm (cat.# 580959 [custom product])
STANDARDS
- EPA 537.1 PFAS calibration standard: 2 µg/mL, methanol (1 mM KOH) (cat.# 30735)
SAMPLE HANDLING
Sample Prep:
- Resprep S-DVB SPE cartridge: 6 mL/500 mg (cat.# 28937)
Sample Reservoirs:
Vacuum Manifolds:
Vacuum Pumps:
- Chemker 300 PTFE vacuum pump: 20 L/min, AC110 V, 60 Hz (cat.# 27426)
- Chemker 300 PTFE vacuum pump: 18 L/min, AC220 V, 50 Hz (cat.# 27427)
GENERAL LAB SUPPLIES
- Polypropylene vials: 100-pk. (cat.# 23243)
- Polypropylene vials: 1000-pk. (cat.# 23246)
- Polyethylene vial caps: 100-pk. (cat.# 23244)
- Polyethylene vial caps: 1000-pk. (cat.# 23247)
- Centrifuge tubes: 50 mL, 50-pk. (cat.# 25846)
- Centrifuge tubes: 50 mL, 500-pk. (cat.# 28290)
- Norm-Ject plastic syringe: 10 mL Luer Lock tip (cat.# 22775)
- Norm-Ject plastic syringe: 5 mL Luer Lock tip (cat.# 22774)
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