PFAS Analysis by U.S. EPA Method 8327 for Surface Water, Groundwater, and Wastewater

• Sample Matrix: surface water, groundwater, and wastewater
• Sample Preparation: method refers to SW-846 Methods 3500 and 3600
• Detection Technique: LC-MS/MS

Per- and Polyfluorinated Alkyl Substances (PFAS) by Liquid Chromatography/Tandem Mass Spectrometry (LC-MS/MS)

U.S. EPA Method 8327 is used for the analysis of 24 PFAS in surface water, groundwater, and wastewater. It refers to SW-846 Methods 3500 and 3600 for details on an appropriate sample preparation procedure (e.g., solvent dilution or extraction), and provides example conditions for LC-MS/MS analysis on a C18 column. Method 8327 is part of the SW-846 compendium and notes that SW-846 methods are intended to be guidance methods that contain general information on how to perform an analytical procedure or technique, which a laboratory can use as a basic starting point for generating its own detailed standard operating procedure (SOP), either for its own general use or for a specific project application. The full analyte list and recommended products for PFAS analysis in surface water, groundwater, or wastewater by Method 8327 are provided below. In addition, you can register and directly compare analyte lists and matrices across PFAS analysis methods here or visit www.restek.com/PFAS to explore our technical resources for PFAS analysis.

 

Analyte List for U.S. EPA Method 8327 for PFAS Analysis

PFAS acronyms, compound names, and CAS numbers vary across methods and guidelines, and they also depend on compound form (e.g., neutral vs. salt). The following list uses standardized terminology to allow easy comparison across the methods and guidelines in our comprehensive guide. Please refer to U.S. EPA Method 8327 for method-specific nomenclature.

Acronym	Compound Name	CAS#
NEtFOSAA	N-ethyl perfluorooctanesulfonamidoacetic acid	2991-50-6
NMeFOSAA	N-methyl perfluorooctanesulfonamidoacetic acid	2355-31-9
PFBS	Perfluoro-1-butanesulfonic acid	375-73-5
PFDA	Perfluorodecanoic acid	335-76-2
PFDoA	Perfluorododecanoic acid	307-55-1
PFHpA	Perfluoroheptanoic acid	375-85-9
PFHxS	Perfluoro-1-hexanesulfonic acid	355-46-4
PFHxA	Perfluorohexanoic acid	307-24-4
PFNA	Perfluorononanoic acid	375-95-1
PFOS	Perfluorooctanesulfonic acid	1763-23-1
PFOA	Perfluorooctanoic acid	335-67-1
PFTeDA	Perfluorotetradecanoic acid	376-06-7
PFTrDA	Perfluorotridecanoic acid	72629-94-8
PFUnA	Perfluoroundecanoic acid	2058-94-8
PFBA	Perfluorobutanoic acid	375-22-4
PFPeA	Perfluoropentanoic acid	2706-90-3
PFDS	Perfluoro-1-decanesulfonic acid	335-77-3
PFHpS	Perfluoro-1-heptanesulfonic acid	375-92-8
PFPeS	Perfluoro-1-pentanesulfonic acid	2706-91-4
FOSA	Perfluoro-1-octanesulfonamide	754-91-6
4:2 FTS	1H,1H,2H,2H-Perfluorohexane sulfonic acid	757124-72-4
6:2 FTS	1H,1H,2H,2H-Perfluorooctane sulfonic acid	27619-97-2
8:2 FTS	1H,1H,2H,2H-Perfluorodecane sulfonic acid	39108-34-4
PFNS	Perfluoro-1-nonanesulfonic acid	68259-12-1

 

Recommended Products for U.S. EPA Method 8327 for PFAS Analysis

ANALYSIS

Analytical LC Columns:

• Raptor C18 SPP column: 50 x 2.1 mm, 2.7 μm (cat.# 9304A52)
• Force C18 FPP column: 50 x 2.1 mm, 1.8 μm (cat.# 9634252)

Delay Columns:

• PFAS delay column: 5 µm, 50 x 2.1 mm (cat.# 27854)
• PFAS delay column: 5 µm, 50 x 3.0 mm (cat.# 580959 [custom product])

STANDARDS

• EPA 537.1 PFAS calibration standard: 2 µg/mL, methanol (1 mM KOH) (cat.# 30735)
• EPA 533 PFAS calibration standard: 2 µg/mL, methanol (1 mM KOH) (cat.# 30736)

SAMPLE HANDLING

Sample Prep:

• 30 mm syringe filter: 0.45 µm, nylon (cat.# 23981)
• 30 mm syringe filter: 0.22 µm, nylon (cat.# 23980)

Vacuum Manifolds:

• Resprep QR-24 vacuum manifold (cat.# 28299-VM)
• Resprep QR-12 vacuum manifold (cat.# 28298-VM)

Vacuum Pumps:

• Chemker 300 PTFE vacuum pump: 20 L/min, AC110 V, 60 Hz (cat.# 27426)
• Chemker 300 PTFE vacuum pump: 18 L/min, AC220 V, 50 Hz (cat.# 27427)

GENERAL LAB SUPPLIES

• Polypropylene vials: 100-pk. (cat.# 23243)
• Polypropylene vials: 1000-pk. (cat.# 23246)
• Polyethylene vial caps: 100-pk. (cat.# 23244)
• Polyethylene vial caps: 1000-pk. (cat.# 23247)
• Centrifuge tubes: 50 mL, 50-pk. (cat.# 25846)
• Centrifuge tubes: 50 mL, 500-pk. (cat.# 28290)
• Norm-Ject plastic syringe: 10 mL Luer Lock tip (cat.# 22775)
• Norm-Ject plastic syringe: 5 mL Luer Lock tip (cat.# 22774)

 

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