PFAS Analysis by U.S. EPA Method OTM-45 for Emissions

• Sample Matrix: emissions from stationary sources
• Sample Preparation: WAX solid-phase extraction
• Detection Technique: LC-MS/MS

Other Test Method 45 (OTM-45) Measurement of Selected Per- and Polyfluorinated Alkyl Substances from Stationary Sources

U.S. EPA Method OTM-45 is a performance-based draft method that can be used for the analysis of 50 PFAS in air emissions from stationary sources. It combines solid-phase extraction sample preparation using weak anion exchange (WAX) cartridges with LC-MS/MS analysis on a C18 column. Draft Method OTM-45 was released to encourage a consistent process for facilities, stationary source test teams, research laboratories, and other stakeholders and to promote discussion and further evaluation of the method. The full analyte list and recommended products for PFAS analysis in emissions by Method OTM-45 are provided below. In addition, you can register and directly compare analyte lists and matrices across PFAS analysis methods here or visit www.restek.com/PFAS to explore our technical resources for PFAS analysis.

 

Analyte List for EPA Method OTM-45 for PFAS Analysis

PFAS acronyms, compound names, and CAS numbers vary across methods and guidelines, and they also depend on compound form (e.g., neutral vs. salt). The following list uses standardized terminology to allow easy comparison across the methods and guidelines in our comprehensive guide. Please refer to U.S. EPA Method OTM-45 for method-specific nomenclature.

Acronym	Compound Name	CAS#
NEtFOSAA	N-Ethyl perfluorooctanesulfonamidoacetic acid	2991-50-6
NMeFOSAA	N-Methyl perfluorooctanesulfonamidoacetic acid	2355-31-9
PFBS	Perfluoro-1-butanesulfonic acid	375-73-5
PFDA	Perfluorodecanoic acid	335-76-2
PFDoA	Perfluorododecanoic acid	307-55-1
PFHpA	Perfluoroheptanoic acid	375-85-9
PFHxS	Perfluoro-1-hexanesulfonic acid	355-46-4
PFHxA	Perfluorohexanoic acid	307-24-4
PFNA	Perfluorononanoic acid	375-95-1
PFOS	Perfluorooctanesulfonic acid	1763-23-1
PFOA	Perfluorooctanoic acid	335-67-1
PFTeDA	Perfluorotetradecanoic acid	376-06-7
PFTrDA	Perfluorotridecanoic acid	72629-94-8
PFUnA	Perfluoroundecanoic acid	2058-94-8
HFPO-DA, GenX	Hexafluoropropylene oxide dimer acid	13252-13-6
PFBA	Perfluorobutanoic acid	375-22-4
PFPeA	Perfluoropentanoic acid	2706-90-3
PFDS	Perfluoro-1-decanesulfonic acid	335-77-3
PFHpS	Perfluoro-1-heptanesulfonic acid	375-92-8
PFPeS	Perfluoro-1-pentanesulfonic acid	2706-91-4
FOSA	Perfluoro-1-octanesulfonamide	754-91-6
4:2 FTS	1H,1H,2H,2H-Perfluorohexane sulfonic acid	757124-72-4
6:2 FTS	1H,1H,2H,2H-Perfluorooctane sulfonic acid	27619-97-2
8:2 FTS	1H,1H,2H,2H-Perfluorodecane sulfonic acid	39108-34-4
10:2 FTS	1H,1H,2H,2H-perfluorododecane sulfonate (10:2)	120226-60-0
8:2 FTUCA, FOUEA	2H-Perfluoro-2-decenoic acid (8:2)	70887-84-2
NMeFOSA	N-Methylperfluorooctanesulfonamide	31506-32-8
NEtFOSA	N-Ethylperfluorooctanesulfonamide	4151-50-2
PFHxDA	Perfluoro-n-hexadecanoic acid	67905-19-5
PFODA	Perfluoro-n-octadecanoic acid	16517-11-6
9Cl-PF3ONS	9-Chlorohexadecafluoro-3-oxanonane-1-sulfonic acid (F-53B Major)	756426-58-1
11Cl-PF3OUdS	11-Chloroeicosafluoro-3-oxaundecane-1-sulfonic acid (F-53B Minor)	763051-92-9
ADONA	4,8-Dioxa-3H-perfluorononanoic acid	919005-14-4
PFNS	Perfluoro-1-nonanesulfonic acid	68259-12-1
PFecHS	Decafluoro-4-(pentafluoroethyl)cyclohexanesulfonate	646-83-3 (67584-42-3)
6:2FTCA, 6:2 FHEA	2-Perfluorohexyl ethanoic acid	53826-12-3
8:2 FOEA	2-Perfluorooctyl ethanoic acid	27854-31-5
10:2 FDEA	2-Perfluorodecyl ethanoic acid	53826-13-4
6:2 FHUEA	2H-Perfluoro-2-octenoic acid	70887-88-6
PFEESA	Perfluoro(2-ethoxyethane)sulfonic acid	113507-82-7
NFDHA	Nonafluoro-3,6-dioxaheptanoic acid	151772-58-6
PFMPA	Perfluoro-3-methoxypropanoic acid	377-73-1
PFMBA	Perfluoro-4-methoxybutanoic acid	863090-89-5
NMeFOSE	2-(N-methylperfluoro-1-octanesulfonamido)-ethanol	24448-09-7
NEtFOSE	2-(N-ethylperfluoro-1-octanesulfonamido)-ethanol	1691-99-2
PFDoS	Perfluorododecane sulfonate	79780-39-5
PFDoS	Sodium perfluoro-1-dodecanesulfonate	1260224-54-1
3:3 FTCA	3:3 Fluorotelomer carboxylic acid	356-02-5
5:3 FTCA	5:3 Fluorotelomer carboxylic acid	914637-49-3
7:3 FTCA, FHpPA	7:3 Fluorotelomer carboxylic acid or 3-perfluoroheptyl propanoic acid	812-70-4

 

 

Recommended Products for U.S. EPA Method OTM-45 for PFAS Analysis

ANALYSIS

Analytical LC Columns:

• Raptor C18 SPP column: 2.7 μm, 50 x 2.1 mm (cat.# 9304A52)
• Force C18 FPP column: 1.8 μm, 50 x 2.1 mm (cat.# 9634252)

Delay Columns:

• PFAS delay column: 5 µm, 50 x 2.1 mm (cat.# 27854)
• PFAS delay column: 5 µm, 50 x 3.0 mm (cat.# 580959 [custom product])

Guard Columns:

• UltraShield UHPLC precolumn filter: 0.2 µm frit (cat.# 25809)
• EXP direct connect holder for EXP guard cartridges (cat.# 25808)
• Raptor C18 EXP guard column cartridge: 2.7 µm, 5 x 2.1 mm (cat.# 9304A0252)

SAMPLE HANDLING

Sample Prep:

• Deactivated borosilicate glass wool (cat.# 20789)
• Resprep polymeric SPE cartridge: WAX, 6 mL/500 mg, 30 µm (cat.# 28268)
• Resprep polymeric SPE cartridge: WAX, 6 mL/200 mg, 30 µm (cat.# 28292)
• Rezin certified clean SDVB resin (cat.# 27242)
• Ultra-Clean resin (cat.# 24230)

Vacuum Manifolds:

• Resprep QR-24 vacuum manifold (cat.# 28299-VM)
• Resprep QR-12 vacuum manifold (cat.# 28298-VM)

Vacuum Pumps:

• Chemker 300 PTFE vacuum pump: 20 L/min, AC110 V, 60 Hz (cat.# 27426)
• Chemker 300 PTFE vacuum pump: 18 L/min, AC220 V, 50 Hz (cat.# 27427)

GENERAL LAB SUPPLIES

• Polypropylene vials: 100-pk. (cat.# 23243)
• Polypropylene vials: 1000-pk. (cat.# 23246)
• Polyethylene vial caps: 100-pk. (cat.# 23244)
• Polyethylene vial caps: 1000-pk. (cat.# 23247)
• Centrifuge tubes: 50 mL, 50-pk. (cat.# 25846)
• Centrifuge tubes: 50 mL, 500-pk. (cat.# 28290)
• Norm-Ject plastic syringe: 10 mL Luer Lock tip (cat.# 22775)
• Norm-Ject plastic syringe: 5 mL Luer Lock tip (cat.# 22774)

 

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