Restek
Home / Resource Hub / Technical Literature Library / PFAS Analysis by US FDA C-010-03 for Food and Feed

PFAS Analysis by U.S. FDA C-010.03 for Food and Feed

articleBanner
  • Sample Matrix: food and feed (validated in lettuce, chocolate milk, salmon, bread, eggs, clams, blueberries, silage, and corn snaplage)
  • Sample Preparation: QuEChERS, filtration, solid-phase extraction
  • Detection Technique: LC-MS/MS

Determination of 30 Per- and Polyfluoroalkyl Substances (PFAS) in Food and Feed using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

U.S. FDA Method C-010.03 is used for the analysis of 30 PFAS in food and feed. It combines QuEChERS, filtration, and solid phase extraction sample preparation with LC-MS/MS analysis on a C18 column. The method also uses LC-HRMS for verification of PFBA and PFPeA detection in order to address the potential for false positives due to matrix interferences since these compounds each have only one MS/MS transition. The full analyte list and recommended products for PFAS analysis in food and feed by Method C-010.03 are provided below. In addition, you can register and directly compare analyte lists and matrices across PFAS analysis methods here or visit www.restek.com/PFAS to explore our technical resources for PFAS analysis.

 

Analyte List for FDA C-010.03 for PFAS Samples

PFAS acronyms, compound names, and CAS numbers vary across methods and guidelines, and they also depend on compound form (e.g., neutral vs. salt). The following list uses standardized terminology to allow easy comparison across the methods and guidelines in our comprehensive guide. Please refer to U.S. FDA C-010.03 for method-specific nomenclature.

Acronym Compound Name CAS#
PFBS Perfluoro-1-butanesulfonic acid 375-73-5
PFDA Perfluorodecanoic acid 335-76-2
PFDoA Perfluorododecanoic acid 307-55-1
PFHpA Perfluoroheptanoic acid 375-85-9
PFHxS Perfluoro-1-hexanesulfonic acid 355-46-4
PFHxA Perfluorohexanoic acid 307-24-4
PFNA Perfluorononanoic acid 375-95-1
PFOS Perfluorooctanesulfonic acid 1763-23-1
PFOA Perfluorooctanoic acid 335-67-1
PFTeDA Perfluorotetradecanoic acid 376-06-7
PFTrDA Perfluorotridecanoic acid 72629-94-8
PFUnA Perfluoroundecanoic acid 2058-94-8
HFPO-DA, GenX Hexafluoropropylene oxide dimer acid 13252-13-6
PFBA Perfluorobutanoic acid 375-22-4
PFPeA Perfluoropentanoic acid 2706-90-3
PFDS Perfluoro-1-decanesulfonic acid 335-77-3
PFHpS Perfluoro-1-heptanesulfonic acid 375-92-8
PFPeS Perfluoro-1-pentanesulfonic acid 2706-91-4
FOSA Perfluoro-1-octanesulfonamide 754-91-6
4:2 FTS 1H,1H,2H,2H-Perfluorohexane sulfonic acid 757124-72-4
6:2 FTS 1H,1H,2H,2H-Perfluorooctane sulfonic acid 27619-97-2
8:2 FTS 1H,1H,2H,2H-Perfluorodecane sulfonic acid 39108-34-4
9Cl-PF3ONS 9-Chlorohexadecafluoro-3-oxanonane-1-sulfonic acid (F-53B Major) 756426-58-1
11Cl-PF3OUdS 11-Chloroeicosafluoro-3-oxaundecane-1-sulfonic acid (F-53B Minor) 763051-92-9
ADONA 4,8-Dioxa-3H-perfluorononanoic acid 919005-14-4
PFNS Perfluoro-1-nonanesulfonic acid 68259-12-1
PFDoS Perfluorododecane sulfonate 79780-39-5
10:2 FTS 1H,1H,2H,2H-perfluorododecane sulfonate (10:2) 120226-60-0
PFUnDS Perfluoroundecane sulfonic acid 749786-16-1
PFTrDS Perfluorotridecane sulfonic acid 791563-89-8

 

Recommended Products for FDA C-010.03 for PFAS Analysis

ANALYSIS

Analytical LC Columns:

  • Raptor C18 SPP column: 50 x 2.1 mm, 2.7 μm (cat.# 9304A52)
  • Force C18 FPP column: 50 x 2.1 mm, 1.8 μm (cat.# 9634252)

Delay Columns:

  • PFAS delay column: 5 µm, 50 x 2.1 mm (cat.# 27854)
  • PFAS delay column: 5 µm, 50 x 3.0 mm (cat.# 580959 [custom product])

SAMPLE HANDLING

Sample Prep:

  • 30 mm syringe filter: 0.45 µm, nylon (cat.# 23981)
  • 30 mm syringe filter: 0.22 µm, nylon (cat.# 23980)
  • Thomson SINGLE StEP nano filter vials: 0.2 µm, nylon w/preslit cap (cat.# 25886) (Note that this U.S. FDA method uses nano filter vial without caps. Cat#. 25886 comes with preslit caps, but polyethylene caps (cat.#s 23244 and 23247) can be ordered separately.)
  • Resprep polymeric SPE cartridge: WAX, 6 mL/500 mg, 30 µm (cat.# 28291)
  • Resprep polymeric SPE cartridge: WAX, 6 mL/200 mg, 30 µm (cat.# 28292)
  • Q-sep QuEChERS extraction salt packets only (original): 6 g MgSO4, 1.5 g NaCl (cat.# 582025)
  • Q-sep multispeed centrifuge for QuEChERS (cat.# 28295)

Vacuum Manifolds:

  • Resprep QR-24 vacuum manifold (cat.# 28299-VM)
  • Resprep QR-12 vacuum manifold (cat.# 28298-VM)

Vacuum Pumps:

  • Chemker 300 PTFE vacuum pump: 20 L/min, AC110 V, 60 Hz (cat.# 27426)
  • Chemker 300 PTFE vacuum pump: 18 L/min, AC220 V, 50 Hz (cat.# 27427)

GENERAL LAB SUPPLIES

  • Polypropylene vials: 100-pk. (cat.# 23243)
  • Polypropylene vials: 1000-pk. (cat.# 23246)
  • Polyethylene vial caps: 100-pk. (cat.# 23244)
  • Polyethylene vial caps: 1000-pk. (cat.# 23247)
  • Centrifuge tubes: 50 mL, 50-pk. (cat.# 25846)
  • Centrifuge tubes: 50 mL, 500-pk. (cat.# 28290)
  • Norm-Ject plastic syringe: 10 mL Luer Lock tip (cat.# 22775)
  • Norm-Ject plastic syringe: 5 mL Luer Lock tip (cat.# 22774)

 

Discover More Solutions for PFAS Analysis!

 

Pro EZLC Chromatogram Modeler Develop PFAS Methods in minutes with our
Pro EZLC Chromatogram Modeler
FSAR4292-UNV