PFAS Analysis by U.S. USDA CLG-PFAS 2.04 for Bovine, Porcine, Poultry, Siluriformes Muscle, and Bovine Plasma
- Sample Matrix: bovine, porcine, poultry, and Siluriformes muscle and bovine plasma
- Sample Preparation: protein precipitation extraction
- Detection Technique: UHPLC-MS/MS
Screening, Determination, and Confirmation of PFAS by UHPLC-MS-MS
USDA Method CLG-PFAS 2.04 is used for the analysis of 16 PFAS in bovine, porcine, poultry, Siluriformes muscle samples, and in bovine plasma samples. It combines a methanol-based protein precipitation extraction with UHPLC-MS/MS analysis on a C18 column. The method is performance based and allows equivalent equipment, reagents, or solutions to be used, and some instrument parameters can be optimized as detailed in the method to improve chromatography and detection. The full analyte list and recommended products for PFAS analysis in bovine, porcine, poultry, Siluriformes muscle, and bovine plasma samples by CLG-PFAS 2.04 are provided below. In addition, you can register and directly compare analyte lists and matrices across PFAS analysis methods here or visit www.restek.com/PFAS to explore our technical resources for PFAS analysis.
Analyte List for USDA CLG-PFAS 2.04 for PFAS Analysis
PFAS acronyms, compound names, and CAS numbers vary across methods and guidelines, and they also depend on compound form (e.g., neutral vs. salt). The following list uses standardized terminology to allow easy comparison across the methods and guidelines in our comprehensive guide. Please refer to U.S. USDA CLG-PFAS 2.04 for method-specific nomenclature.
| Acronym | Compound Name | CAS# |
| PFBS | Perfluoro-1-butanesulfonic acid | 375-73-5 |
| PFDA | Perfluorodecanoic acid | 335-76-2 |
| PFDoA | Perfluorododecanoic acid | 307-55-1 |
| PFHpA | Perfluoroheptanoic acid | 375-85-9 |
| PFHxS | Perfluoro-1-hexanesulfonic acid | 355-46-4 |
| PFHxA | Perfluorohexanoic acid | 307-24-4 |
| PFNA | Perfluorononanoic acid | 375-95-1 |
| PFOS | Perfluorooctanesulfonic acid | 1763-23-1 |
| PFOA | Perfluorooctanoic acid | 335-67-1 |
| PFTeDA | Perfluorotetradecanoic acid | 376-06-7 |
| PFTrDA | Perfluorotridecanoic acid | 72629-94-8 |
| PFUnA | Perfluoroundecanoic acid | 2058-94-8 |
| PFPeA | Perfluoropentanoic acid | 2706-90-3 |
| PFDS | Perfluoro-1-decanesulfonic acid | 335-77-3 |
| PFHxDA | Perfluoro-n-hexadecanoic acid | 67905-19-5 |
| PFODA | Perfluoro-n-octadecanoic acid | 16517-11-6 |
Recommended Products for U.S. USDA CLG-PFAS for PFAS Analysis
ANALYSIS
Analytical LC Columns:
- Raptor C18 SPP column: 50 x 2.1 mm, 2.7 μm (cat.# 9304A52)
- Force C18 FPP column: 50 x 2.1 mm, 1.8 μm (cat.# 9634252)
Delay Columns:
- PFAS delay column: 5 µm, 50 x 2.1 mm (cat.# 27854)
- PFAS delay column: 5 µm, 50 x 3.0 mm (cat.# 580959 [custom product])
SAMPLE HANDLING
Sample Prep:
-
Q-sep multispeed centrifuge for QuEChERS (cat.# 28295)
Sample Reservoirs:
Vacuum Manifolds:
Vacuum Pumps:
- Chemker 300 PTFE vacuum pump: 20 L/min, AC110 V, 60 Hz (cat.# 27426)
- Chemker 300 PTFE vacuum pump: 18 L/min, AC220 V, 50 Hz (cat.# 27427)
GENERAL LAB SUPPLIES
- Polypropylene vials: 100-pk. (cat.# 23243)
- Polypropylene vials: 1000-pk. (cat.# 23246)
- Polyethylene vial caps: 100-pk. (cat.# 23244)
- Polyethylene vial caps: 1000-pk. (cat.# 23247)
- Centrifuge tubes: 50 mL, 50-pk. (cat.# 25846)
- Centrifuge tubes: 50 mL, 500-pk. (cat.# 28290)
- Norm-Ject plastic syringe: 10 mL Luer Lock tip (cat.# 22775)
- Norm-Ject plastic syringe: 5 mL Luer Lock tip (cat.# 22774)
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