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Rapid LPGC-MS Analysis of Arylamines Increases Throughput and Reduces Helium Use

Featured Application: Arylamines on LPGC Rxi-35Sil MS

  • Analyze arylamines 3.3x faster than conventional GC-MS.
  • Cut costs by reducing helium consumption 81%.
  • Pre-connected LPGC column kit prevents leaks, simplifies installation, and allows large-volume injection.

Azo dyes are synthetically produced colorants that are commonly used in the food, cosmetic, pharmaceutical, and textile industries. They are present in a wide variety of consumer products, and some can produce carcinogenic or mutagenic arylamines as breakdown products. Due to concerns about human health and exposure, effective methods are needed for the analysis of arylamines. Testing is usually performed by conventional GC-MS or LC-MS, but long analysis times limit sample throughput. 

A much faster alternative, low-pressure GC-MS (LPGC-MS) is a paired-column technique that can bring significant speed gains to labs analyzing arylamines. This approach uses the MS vacuum to lower pressure within the analytical column, thus increasing speed. For example, in the analysis of arylamines (22 of which are regulated by EU REACH legislation [1]) shown in Figure 1, the run time was 3.3x faster than the conventional method, and it also used 81% less helium, which provides a substantial cost savings. 

LPGC-MS only works because the inlet pressure is maintained by pairing the analytical column to a narrow restrictor column. However, due to the difference in tubing diameters, it can be difficult to make a robust connection manually, which has limited adoption of the technique. LPGC column kits from Restek solve this problem because they are factory connected and individually leak checked, which ensures a leak-free connection and simplifies setup. Visit to learn more about this powerful technique.


  1. Regulation (EC) No. 1907/2006 of the European Parliament and of the Council of 18 December 2006 Concerning the Registration, Evaluation, Authorization and Restriction of Chemicals (REACH).

Figure 1: Arylamines on LPGC Rxi-35Sil MS Compared to Conventional GC-MS Analysis