Roc HPLC Columns: The Reliable Cornerstone of Your LC Lab
Conventional HPLC columns built for solid performance, exceptional value, and compact storage.
- Conventional HPLC column built to be the cornerstone for your lab; pressure rated for any 400 bar HPLC system.
- Solid and reliable—delivers the peak shape, reproducibility, ruggedness, and performance you demand.
- Exceptional value for routine analyses and large-volume workflows—high-purity, fully porous silica backed by quality manufacturing features a variety of phases to fit most HPLC methods.
- Ideal for a wide range of applications from food to pharma; 5 phases cover 98% of USP methods.
- Newly designed minimal packaging for compact storage—download serial #-specific certificates of analysis at restek.com/documentation
Roc Family of HPLC Phases
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USP Phase Code |
L1 |
L7 |
L11 |
L10 |
L3 |
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Stationary Phase Category |
C18, octadecylsilane |
C8, octylsilane |
phenyl |
cyano |
bare silica |
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Ligand Type |
monomeric C18 |
monomeric C8 |
phenyl-hexyl |
cyanopropyl |
n/a |
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Particle Size |
3 μm or 5 μm, spherical |
3 μm or 5 μm, spherical |
3 μm or 5 μm, spherical |
3 μm or 5 μm, spherical |
3 μm or 5 μm, spherical |
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Pore Size |
100 Å |
100 Å |
100 Å |
100 Å |
100 Å |
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Surface Area |
300 m2/g |
300 m2/g |
300 m2/g |
300 m2/g |
300 m2/g |
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Carbon Load |
20% |
12% |
15% |
8% |
n/a |
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End-Cap |
yes |
yes |
yes |
yes |
n/a |
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pH Range |
2.5 to 8.0 |
2.5 to 8.0 |
2.0 to 8.0 |
2.5 to 8.0 |
2.5 to 8.0 |
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Maximum Temperature |
80 °C |
80 °C |
80 °C |
80 °C |
80 °C |
Figure 1: Over 1,000 injections and Roc HPLC columns will still offer reliable performance, regardless of which dimension your method calls for.
Figure 2: Add a Roc HPLC guard to further extend the life of your analytical column without impacting your retention, peak shape, or efficiency.
Figure 3: From one column to the next, one lot to the next, Roc columns provide results you can trust time and time again.
Reversed Phase
Normal Phase
Roc HPLC Columns from Restek Perform Better Than or Equal To Columns from Other Vendors
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Columns |
Analyte |
tR |
Area |
Height |
USP Tailing |
Asymmetry (10%) |
k' |
Plate Number |
Resolution |
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Roc |
Ethylparaben |
3.252 |
1,407,609 |
281,358 |
1.176 |
1.148 |
1.182 |
9,738 |
12.5 |
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Ethinyl Estradiol |
5.410 |
1,869,684 |
234,346 |
1.075 |
1.064 |
2.631 |
10,264 |
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Vendor A |
Ethylparaben |
3.060 |
1,424,621 |
215,698 |
1.907 |
1.608 |
1.013 |
6,732 |
10.1 |
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Ethinyl Estradiol |
4.889 |
1,860,659 |
223,879 |
1.311 |
1.217 |
2.217 |
8,575 |
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Vendor B |
Ethylparaben |
2.873 |
1,426,068 |
319,850 |
1.143 |
1.124 |
0.866 |
9,706 |
9.9 |
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Ethinyl Estradiol |
4.307 |
1,867,049 |
287,414 |
1.064 |
1.057 |
1.797 |
10,031 |
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Vendor C |
Ethylparaben |
2.763 |
1,413,099 |
315,080 |
1.262 |
1.217 |
0.932 |
8,643 |
11.9 |
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Ethinyl Estradiol |
4.580 |
1,868,724 |
270,662 |
1.143 |
1.122 |
2.203 |
9,700 |
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Vendor D |
Ethylparaben |
3.157 |
2,939,187 |
357,541 |
1.159 |
0.965 |
1.084 |
4,478 |
12.2 |
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Ethinyl Estradiol |
5.816 |
1,771,785 |
182,056 |
1.301 |
1.232 |
2.839 |
8,954 |
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Vendor E |
Ethylparaben |
3.873 |
1,498,446 |
244,642 |
1.172 |
1.134 |
1.221 |
9,348 |
13.7 |
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Ethinyl Estradiol |
6.839 |
1,781,453 |
174,955 |
1.080 |
1.063 |
2.921 |
9,963 |
Figure 4: Count on Roc HPLC columns from Restek for outstanding chromatographic performance.
Roc HPLC Column
Vendor A
Vendor B
Vendor C
Vendor D
Vendor E
Chromatographic conditions for Table I and Figure 4
All columns are C18 stationary phase (5 µm, 150 x 4.6 mm). Conditions are as follows. Mobile phase: water:acetonitrile (1:1); Flow rate: 1 mL/min; Injection volume: 25 µL; Instrument: HPLC with UV detector, 280 nm; Sample: 200 µg/mL ethinyl estradiol and 50 µg/mL ethylparaben in water:acetonitrile (1:1).