Roc HPLC Columns: The Reliable Cornerstone of Your LC Lab
Conventional HPLC columns built for solid performance, exceptional value, and compact storage.
- Conventional HPLC column built to be the cornerstone for your lab; pressure rated for any 400 bar HPLC system.
- Solid and reliable—delivers the peak shape, reproducibility, ruggedness, and performance you demand.
- Exceptional value for routine analyses and large-volume workflows—high-purity, fully porous silica backed by quality manufacturing features a variety of phases to fit most HPLC methods.
- Ideal for a wide range of applications from food to pharma; 5 phases cover 98% of USP methods.
- Newly designed minimal packaging for compact storage—download serial #-specific certificates of analysis at restek.com/documentation
Roc Family of HPLC Phases
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USP Phase Code |
L1 |
L7 |
L11 |
L10 |
L3 |
Stationary Phase Category |
C18, octadecylsilane |
C8, octylsilane |
phenyl |
cyano |
bare silica |
Ligand Type |
monomeric C18 |
monomeric C8 |
phenyl-hexyl |
cyanopropyl |
n/a |
Particle Size |
3 μm or 5 μm, spherical |
3 μm or 5 μm, spherical |
3 μm or 5 μm, spherical |
3 μm or 5 μm, spherical |
3 μm or 5 μm, spherical |
Pore Size |
100 Å |
100 Å |
100 Å |
100 Å |
100 Å |
Surface Area |
300 m2/g |
300 m2/g |
300 m2/g |
300 m2/g |
300 m2/g |
Carbon Load |
20% |
12% |
15% |
8% |
n/a |
End-Cap |
yes |
yes |
yes |
yes |
n/a |
pH Range |
2.5 to 8.0 |
2.5 to 8.0 |
2.0 to 8.0 |
2.5 to 8.0 |
2.5 to 8.0 |
Maximum Temperature |
80 °C |
80 °C |
80 °C |
80 °C |
80 °C |
Figure 1: Over 1,000 injections and Roc HPLC columns will still offer reliable performance, regardless of which dimension your method calls for.
Figure 2: Add a Roc HPLC guard to further extend the life of your analytical column without impacting your retention, peak shape, or efficiency.

Peaks | Conc. (mg/mL) | |
---|---|---|
1. | Uracil | 0.02 |
2. | Toluene | 3.00 |
3. | Naphthalene | 0.50 |
4. | Biphenyl | 0.06 |
Column | Roc C8 (cat.# 9533565) | ||||||||||||
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Dimensions: | 150 mm x 4.6 mm ID | ||||||||||||
Particle Size: | 5 µm | ||||||||||||
Pore Size: | 100 Å | ||||||||||||
Guard Column: | Roc C8 10 mm, 4.0 mm ID, 5 µm (cat.# 953350210) | ||||||||||||
Temp.: | 30 °C | ||||||||||||
Standard/Sample | Custom mix | ||||||||||||
Diluent: | 50:50 Acetonitrile:water | ||||||||||||
Inj. Vol.: | 2 µL | ||||||||||||
Mobile Phase | |||||||||||||
A: | Water | ||||||||||||
B: | Acetonitrile | ||||||||||||
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Detector | UV @ 254 nm |
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Instrument | HPLC |
Figure 3: From one column to the next, one lot to the next, Roc columns provide results you can trust time and time again.
Reversed Phase

Peaks | tR (min) | |
---|---|---|
1. | p-Tolunitrile | 3.7 |
2. | 4-Butylbenzoic acid | 5.2 |
3. | 3,4-Dimethylanisole | 7.3 |
4. | Mefenamic acid | 8.1 |
5. | p-Xylene | 9.7 |
Column | Roc C18 (cat.# 9534565) |
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Dimensions: | 150 mm x 4.6 mm ID |
Particle Size: | 5 µm |
Pore Size: | 100 Å |
Temp.: | 35 °C |
Standard/Sample | |
Inj. Vol.: | 5 µL |
Mobile Phase | 0.05% Formic acid in water:0.05% Formic acid in acetonitrile (40:60) |
Flow: | 1.0 mL/min |
Detector | UV/Vis @ 220 nm |
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Cell Temp: | 35 °C |
Instrument | HPLC |
Normal Phase

Peaks | tR (min) | |
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1. | Benzene | 3.5 |
2. | Nitrobenzene | 4.1 |
3. | 1,3,5-Trinitrobenzene | 5.5 |
4. | 2,4-Dinitrotoluene | 7.4 |
Column | Roc Cyano (cat.# 953631E) |
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Dimensions: | 100 mm x 3.0 mm ID |
Particle Size: | 3 µm |
Pore Size: | 100 Å |
Temp.: | 40 °C |
Standard/Sample | |
Inj. Vol.: | 1 µL |
Mobile Phase | Water:acetonitrile (80:20) |
Flow: | 0.6 mL/min |
Detector | UV/Vis @ 254 nm |
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Cell Temp: | 40 °C |
Instrument | HPLC |
Roc HPLC Columns from Restek Perform Better Than or Equal To Columns from Other Vendors
Columns |
Analyte |
tR |
Area |
Height |
USP Tailing |
Asymmetry (10%) |
k' |
Plate Number |
Resolution |
Roc |
Ethylparaben |
3.252 |
1,407,609 |
281,358 |
1.176 |
1.148 |
1.182 |
9,738 |
12.5 |
Ethinyl Estradiol |
5.410 |
1,869,684 |
234,346 |
1.075 |
1.064 |
2.631 |
10,264 |
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Vendor A |
Ethylparaben |
3.060 |
1,424,621 |
215,698 |
1.907 |
1.608 |
1.013 |
6,732 |
10.1 |
Ethinyl Estradiol |
4.889 |
1,860,659 |
223,879 |
1.311 |
1.217 |
2.217 |
8,575 |
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Vendor B |
Ethylparaben |
2.873 |
1,426,068 |
319,850 |
1.143 |
1.124 |
0.866 |
9,706 |
9.9 |
Ethinyl Estradiol |
4.307 |
1,867,049 |
287,414 |
1.064 |
1.057 |
1.797 |
10,031 |
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Vendor C |
Ethylparaben |
2.763 |
1,413,099 |
315,080 |
1.262 |
1.217 |
0.932 |
8,643 |
11.9 |
Ethinyl Estradiol |
4.580 |
1,868,724 |
270,662 |
1.143 |
1.122 |
2.203 |
9,700 |
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Vendor D |
Ethylparaben |
3.157 |
2,939,187 |
357,541 |
1.159 |
0.965 |
1.084 |
4,478 |
12.2 |
Ethinyl Estradiol |
5.816 |
1,771,785 |
182,056 |
1.301 |
1.232 |
2.839 |
8,954 |
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Vendor E |
Ethylparaben |
3.873 |
1,498,446 |
244,642 |
1.172 |
1.134 |
1.221 |
9,348 |
13.7 |
Ethinyl Estradiol |
6.839 |
1,781,453 |
174,955 |
1.080 |
1.063 |
2.921 |
9,963 |
Figure 4: Count on Roc HPLC columns from Restek for outstanding chromatographic performance.
Roc HPLC Column
Vendor A
Vendor B
Vendor C
Vendor D
Vendor E
Chromatographic conditions for Table I and Figure 4
All columns are C18 stationary phase (5 µm, 150 x 4.6 mm). Conditions are as follows. Mobile phase: water:acetonitrile (1:1); Flow rate: 1 mL/min; Injection volume: 25 µL; Instrument: HPLC with UV detector, 280 nm; Sample: 200 µg/mL ethinyl estradiol and 50 µg/mL ethylparaben in water:acetonitrile (1:1).