Rugged, Reliable Raptor Columns with the Speed of C8

• Less retention of nonpolar analytes for faster analysis times.
• Proprietary column packing of superficially porous particles (SPP) and premium hardware.
• Superior performance in cannabis, food, clinical, and environmental testing.

Restek’s Raptor C8 columns boast the dependability of our Raptor line with the capability of achieving even faster analysis times compared to C18 columns. Defined by superior ruggedness and reproducibility, Raptor columns are the go-to choice for even the most challenging workflows.

Featuring SPP "core-shell" technology and a highly-selective monomeric octylsilane phase, our Raptor C8 column is a high-performing, general-purpose column for cannabis, food, clinical, and environmental testing. With a shorter alkyl chain than a traditional C18 column, this Raptor C8 boasts increased analysis speeds and improved peak shape. Like all of our Raptor columns, this Raptor C8 is exceptionally durable with long column lifetimes and dependable reproducibility.

Learn more about our Raptor column line.

Raptor C8 Column Description:
	Stationary Phase Category: C8, octylsilane (L7) Ligand Type: C8 Particle: 2.7 µm superficially porous particle Pore Size: 90 Å Surface Area: 130 m2/g	Recommended Usage: pH Range: 2.0–8.0 Maximum Temperature: 80 °C Maximum Pressure: 600 bar/8700 ps Properties: Similar selectivity to C18 with less retention of nonpolar analytes for faster analysis times. Part of Restek’s Raptor LC column line featuring 2.7 µm SPP core-shell silica. When to switch to C8: You want to achieve faster analysis times.  Want less retention of non-polar analytes.
Column Interaction Profile: Defining Solute Interactions: Dispersion		Solute Retention Profile: Target Analyte Structures: Hydrocarbons  Target Analyte Functionalities: Hydrophobic compounds

PEth Homologues in Whole Blood on Raptor C8: Excellent Column Lifetimes and Lot-to-Lot Reproducibility with No Carryover

Using the Raptor C8 column, a robust and accurate analysis of the two most predominant phosphatidylethanol (PEth) homologues in whole blood was developed using a simple protein precipitation. PEth compounds are prone to carryover, but when utilizing the Raptor C8 column, no carryover was detected (Figure 1). Additionally, the Raptor C8 column demonstrated long column lifetimes and exceptional reproducibility from lot-to-lot (Figures 2 and 3).

View the full PEth application here. 

Figure 1: Carryover is a common challenge in PEth analysis, but when utilizing the Raptor C8 column, no carryover was detected following a blank injection after an injection of the highest calibrator standard.

	Peaks	tR (min)	Precursor Ion	Product Ion 1	Product Ion 2
1.	Phosphatidylethanol 16:0/18:2	2.37	699.50	279.25	255.15
2.	Phosphatidylethanol 16:0/18:2-d5	2.37	704.50	279.35	-
3.	Phosphatidylethanol 16:0/18:1	2.63	701.00	281.25	255.20
4.	Phosphatidylethanol 16:0/18:1-d5	2.63	706.50	281.15	-

Column	Raptor C8 (cat.# 9303A52)
Dimensions:	50 mm x 2.1 mm ID
Particle Size:	2.7 µm
Pore Size:	90 Å
Guard Column:	Raptor C8 EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9303A0252)
Temp.:	30 °C
Standard/Sample
Diluent:	4:1 2-propanol:tetrahydrofuran
Conc.:	1000 ng/mL in whole blood
Inj. Vol.:	5 µL
Mobile Phase
A:	5 mM Ammonium acetate, water
B:	90:10 Acetonitrile:2-propanol
	Time (min) Flow (mL/min) %A %B 0.00 0.6 30 70 2.50 0.6 10 90 3.00 0.6 5 95 3.01 1.0 5 95 3.50 1.0 0 100 3.51 0.6 30 70 5.00 0.6 30 70
Max Pressure:	210 bar

Detector	Shimadzu 8060 MS/MS
Ion Source:	Electrospray
Ion Mode:	ESI-
Mode:	MRM
Instrument	Shimadzu Nexera X2
Sample Preparation	Procedure provided by RedHot Diagnostics: The fortified blood sample (50 µL) was mixed with 50 µL of internal standard and 150 µL of 4:1 2-propanol:tetrahydrofuran. The mixture was vortexed for 20 seconds and centrifuged for 10 minutes at 4300 rpm. The supernatant was aliquoted to a 2 mL screw-thread vial (cat.# 21143) with a glass insert (cat.# 21776) and capped with short-cap, screw-vial closure (cat.# 24498). Five microliters was injected for analysis.

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Figure 2: The Raptor C8 column boasts superior column lifetimes. Exceptional peak shape and response are maintained even after 500 injections.

	Peaks	Precursor Ion	Product Ion 1	Product Ion 2
1.	Phosphatidylethanol 16:0/18:2	699.50	279.25	255.15

Column	Raptor C8 (cat.# 9303A52)
Dimensions:	50 mm x 2.1 mm ID
Particle Size:	2.7 µm
Pore Size:	90 Å
Guard Column:	Raptor C8 EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9303A0252)
Temp.:	30 °C
Standard/Sample
Diluent:	4:1 2-propanol:tetrahydrofuran
Conc.:	100 ng/mL in whole blood
Inj. Vol.:	5 µL
Mobile Phase
A:	5 mM Ammonium acetate, water
B:	90:10 Acetonitrile:2-propanol
	Time (min) Flow (mL/min) %A %B 0.00 0.6 30 70 2.50 0.6 10 90 3.00 0.6 5 95 3.01 1.0 5 95 3.50 1.0 0 100 3.51 0.6 30 70 5.00 0.6 30 70
Max Pressure:	210 bar

Detector	Shimadzu 8060 MS/MS
Ion Source:	Electrospray
Ion Mode:	ESI-
Mode:	MRM
Instrument	Shimadzu Nexera X2
Sample Preparation	Procedure provided by RedHot Diagnostics: The fortified blood sample (50 µL) was mixed with 50 µL of internal standard and 150 µL of 4:1 2-propanol:tetrahydrofuran. The mixture was vortexed for 20 seconds and centrifuged for 10 minutes at 4300 rpm. The supernatant was aliquoted to a 2 mL screw-thread vial (cat.# 21143) with a glass insert (cat.# 21776) and capped with a short-cap, screw-vial closure (cat.# 24498). Five microliters was injected for analysis.

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Figure 3: From one lot to the next, every Raptor C8 column you purchase will perform the same.

	Peaks	Precursor Ion	Product Ion 1	Product Ion 2
1.	Phosphatidylethanol 16:0/18:2	699.50	279.25	255.15

Column	Raptor C8 (cat.# 9303A52)
Dimensions:	50 mm x 2.1 mm ID
Particle Size:	2.7 µm
Pore Size:	90 Å
Guard Column:	Raptor C8 EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9303A0252)
Temp.:	30 °C
Standard/Sample
Diluent:	4:1 2-propanol:tetrahydrofuran
Conc.:	100 ng/mL in whole blood
Inj. Vol.:	5 µL
Mobile Phase
A:	5 mM Ammonium acetate, water
B:	90:10 Acetonitrile:2-propanol
	Time (min) Flow (mL/min) %A %B 0.00 0.6 30 70 2.50 0.6 10 90 3.00 0.6 5 95 3.01 1.0 5 95 3.50 1.0 0 100 3.51 0.6 30 70 5.00 0.6 30 70
Max Pressure:	210 bar

Detector	Shimadzu 8060 MS/MS
Ion Source:	Electrospray
Ion Mode:	ESI-
Mode:	MRM
Instrument	Shimadzu Nexera X2
Sample Preparation	Procedure provided by RedHot Diagnostics: The fortified blood sample (50 µL) was mixed with 50 µL of internal standard and 150 µL of 4:1 2-propanol:tetrahydrofuran. The mixture was vortexed for 20 seconds and centrifuged for 10 minutes at 4300 rpm. The supernatant was aliquoted to a 2 mL screw-thread vial (cat.# 21143) with a glass insert (cat.# 21776) and capped with a short-cap, screw-vial closure (cat.# 24498). Five microliters was injected for analysis.

Download PDF