According to My Calculations: Chromatographic Calculators Help Take the Guesswork Out
17 May 2009Quick! Your oven is at 50 °C, your column is 30 m long with an ID of 0.32 mm. You have an inlet pressure of 10 psi (about 70 kPa) and temperature of 250 °C (He as your carrier). Finally, you’re using a 4 mm ID, 78.5 mm long straight inlet liner. What should your splitless hold time be? Time’s up...do you have the answer? No? Whew...then I’m not the only one who would struggle doing that in my head. Thankfully, none of you should have to do that calculation in your head (but if you do, bravo!). There are calculators that help you figure out these important parameters without ever making an injection. Restek has some, and you can find them via the link below.