When should I use LC Passivation Solution22 Sep 2022
As more analyses are being done for polar compounds, analysts are increasingly seeing the need to passivate their LC systems and columns for their analysis. We sell passivation solution as catalog #32475. While some situations warrant such a treatment up front before the analysis is attempted, other applications may be more of a “wait and see” scenario.
Analytes are often phosphorylated compounds like glyphosate, since they are known to easily chelate with metal in an LC system. Passivation is occasionally needed for other types of analysis also, such as for the antiviral drug Remdesivir. Some columns, such as Restek’s Raptor Polar X columns, are passivated prior to shipping, but usually LC instruments need to be treated also.
(The above image was presented at the 2020 Environmental Measurement Symposium (NEMC), the NELAC Institute.)
It is also important to note that passivation is meant to be applied for a limited period of time. Continued exposure to these ingredients over an extended period of time, particularly if they are added to the mobile phase, can result in damage to an LC column. Please contact us if you find the need to apply the passivation beyond what is described in the instruction sheet.
- LC-MS/MS Analysis of Glyphosate & Other Polar Contaminants in Food with an Ion Exchange/HILIC Column (video)
- Antiviral Analysis: Remdesivir and its Metabolites (blog post)
We hope you find this useful and thank you for reading.