Pure Chromatography
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Derivatized Cholesterol on Rxi-5ms (Temp. Program)
Peaks | |
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1. | 5-α-Cholestane (IS)![]() |
Peaks | |
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2. | Cholesterol![]() |

GC_FF00883
Column | Rxi-5ms, 15 m, 0.25 mm ID, 0.25 µm (cat.# 13420) |
Sample | 1,000 μg/mL cholesterol in hexane |
1,000 μg/mL 5-α-cholestane in hexane | |
50 ng derivatized cholesterol, 150 ng 5-α-cholestane on column | |
Injection | |
Inj. Vol.: | 1 µL split (split ratio 20:1) |
Liner: | Splitless taper (4 mm) w/wool (cat.# 22405) |
Inj. Temp.: | 250 °C |
Oven | |
Oven Temp.: | 200 °C (hold 1 min) to 330 °C at 20 °C/min (hold 7.5 min) |
Carrier Gas | He, constant pressure (9.7 psi, 66.9 kPa) |
Linear Velocity: | 24 cm/sec @ 200 °C |
Detector | FID @ 340 °C |
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