Pure Chromatography
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Derivatized Cholesterol on Rxi-5ms (Temp. Program)
| Peaks | |
|---|---|
| 1. | 5-α-Cholestane (IS) |
| Peaks | |
|---|---|
| 2. | Cholesterol |
GC_FF00883
| Column | Rxi-5ms, 15 m, 0.25 mm ID, 0.25 µm (cat.# 13420) |
| Sample | 1,000 μg/mL cholesterol in hexane |
| 1,000 μg/mL 5-α-cholestane in hexane | |
| 50 ng derivatized cholesterol, 150 ng 5-α-cholestane on column | |
| Injection | |
| Inj. Vol.: | 1 µL split (split ratio 20:1) |
| Liner: | Splitless taper (4 mm) w/wool (cat.# 22405) |
| Inj. Temp.: | 250 °C |
| Oven | |
| Oven Temp.: | 200 °C (hold 1 min) to 330 °C at 20 °C/min (hold 7.5 min) |
| Carrier Gas | He, constant pressure (9.7 psi, 66.9 kPa) |
| Linear Velocity: | 24 cm/sec @ 200 °C |
| Detector | FID @ 340 °C |
RELATED CHROMATOGRAM LIBRARY SEARCHES
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Restek Corporation, U.S., 110 Benner Circle, Bellefonte, PA 16823
Copyright © 2018 Restek Corporation. All rights reserved.
A Company of Owners
Cookies allow for a variety of features that make your visit to Restek more enjoyable. By using this website, you agree to the use of cookies in accordance with our guidelines. For more information, see our privacy policy.