Pure Chromatography
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Rxi-65TG: High-Temperature Stability Overlay
| Peaks | |
|---|---|
| 1. | Tripalmitin (PPP) |
| 2. | 1,2-Palmitin-3-stearin (PPS) |
| 3. | 1,3-Palmitin-2-olein (POP) |
| 4. | 1,2-Palmitin-3-linolein (PPL) |
| 5. | 1-Palmitin-2-olein-3-stearin (POS) |
| Peaks | |
|---|---|
| 6. | 1,2-Olein-3-palmitin (POO) |
| 7. | 1-Palmitin-2-linolein-3-olein (PLO) |
| 8. | 1,2-Stearin-3-olein (SSO) |
| 9. | Triolein (OOO) |
| 10. | 1,2-Olein-3-linolein (OOL) |
GC_FF1287
| Column | Rxi-65TG, 30 m, 0.25 mm ID, 0.1 µm (cat.# 17108) |
| Sample | Palm oil analytical standard |
| Mono/diacylglycerol custom mix | |
| Diluent: | Isooctane |
| Injection | |
| Inj. Vol.: | 1 µL split (split ratio 100:1) |
| Liner: | Topaz 4.0 mm ID Precision inlet liner w/wool (cat.# 23305) |
| Inj. Temp.: | 360 °C |
| Split Vent Flow Rate: | 200 mL/min |
| Oven | |
| Carrier Gas | H2, constant flow |
| Flow Rate: | 2 mL/min |
| Detector | FID @ 360 °C |
| Make-up Gas Flow Rate: | 30 mL/min |
| Make-up Gas Type: | N2 |
| Hydrogen flow: | 40 mL/min |
| Air flow: | 370 mL/min |
| Data Rate: | 50 Hz |
| Instrument | Agilent 7890B GC |
| Notes | Sample prep: ~50 mg of a palm oil standard (which contains naturally occurring triglycerides) was fortified with the following mono- and diacylglycerol standards. The mixture was then brought up to 10 mL in isooctane, giving a final mono- and diglycerides concentration of ~100 μg/mL. 1. 1,2-Dipalmitin 2. 1,3-Dipalmitin 3. 1,2-Distearin 4. 1,3-Distearin 5. 1-Monopalmitin 6. 2-Monopalmitin 7. 1-Monostearin Testing Conditions: Six injections of fortified palm oil were made under the following conditions. Extended final hold times were used to challenge column stability. After each 24-hour challenge period, column performance was assessed using an injection with a final hold time that is typical for triglyceride analysis. Injection 1: 100 °C (hold 1 min) to 325 °C at 30 °C/min to 345 °C at 1 °C/min to 360 °C at 5 °C/min (hold 12 hr) Injection 2: 100 °C (hold 1 min) to 325 °C at 30 °C/min to 345 °C at 1 °C/min to 360 °C at 5 °C/min (hold 12 hr) Injection 3: 100 °C (hold 1 min) to 325 °C at 30 °C/min to 345 °C at 1 °C/min to 360 °C at 5 °C/min (hold 5 min) Injection 4: 100 °C (hold 1 min) to 325 °C at 30 °C/min to 345 °C at 1 °C/min to 360 °C at 5 °C/min (hold 12 hr) Injection 5: 100 °C (hold 1 min) to 325 °C at 30 °C/min to 345 °C at 1 °C/min to 360 °C at 5 °C/min (hold 12 hr) Injection 6: 100 °C (hold 1 min) to 325 °C at 30 °C/min to 345 °C at 1 °C/min to 360 °C at 5 °C/min (hold 5 min) |
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Restek Corporation, U.S., 110 Benner Circle, Bellefonte, PA 16823
Copyright © 2020 Restek Corporation. All rights reserved.
A Company of Owners
Cookies allow for a variety of features that make your visit to Restek more enjoyable. By using this website, you agree to the use of cookies in accordance with our guidelines. For more information, see our privacy policy.