share:
delim
delim
Food Industry FAMEs on Rt-2560 by AOAC Method 996.06 Using H2

Food Industry FAMEs on Rt-2560 by AOAC Method 996.06 Using H2

PeakstR (min)Conc. (µg/mL)Structural Nomenclature
1.Methyl butyrate6.621400C4:0
2.Methyl caproate7.711400C6:0
3.Methyl octanoate9.556400C8:0
4.Methyl decanoate12.094400C10:0
5.Methyl undecanoate13.506200C11:0
6.Methyl dodecanoate14.943400C12:0
7.Methyl tridecanoate16.372200C13:0
8.Methyl myristate17.772400C14:0
9.Methyl myristoleate18.916200C14:1 (c9)
10.Methyl pentadecanoate19.127200C15:0
11.Methyl pentadecenoate20.252200C15:1 (c10)
12.Methyl palmitate20.436600C16:0
13.Methyl palmitoleate21.361200C16:1 (c9)
14.Methyl heptadecanoate21.691200C17:0
15.Methyl heptadecenoate22.595200C17:1 (c10)
16.Methyl stearate22.899400C18:0
17.Methyl octadecenoate23.442200C18:1 (t9)
18.Methyl oleate23.677400C18:1 (c9)
19.Methyl linolelaidate24.292200C18:2 (t9,t12)
20.Methyl linoleate24.811200C18:2 (c9,c12)
21.Methyl arachidate25.171400C20:0
22.Methyl linolenate25.637200C18:3 (c6,c9,c12)
23.Methyl eicosenoate25.903200C20:1 (c11)
24.Methyl linolenate26.081200C18:3 (c9,c12,c15)
25.Methyl heneicosanoate26.239200C21:0
26.Methyl eicosadienoate26.980200C20:2 (c11,c14)
27.Methyl behenate27.274400C22:0
28.Methyl eicosatrienoate27.763200C20:3 (c8,c11,c14)
29.Methyl erucate27.981200C22:1 (c13)
30.Methyl eicosatrienoate28.190200C20:3 (c11,c14,c17)
31.Methyl arachidonate28.286200C20:4 (c5,c8,c11,c14)
32.Methyl tricosanoate28.381200C23:0
33.Methyl docosadienoate29.071200C22:2 (c13,c16)
34.Methyl lignocerate29.341400C24:0
35.Methyl eicosapentaenoate29.737200C20:5 (c5,c8,c11,c14,c17)
36.Methyl nervonate30.131200C24:1 (C15)
37.Methyl docosahexaenoate33.132200C22:6 (c4,c7,c10,c13,c16,c19)
image
GC_FF1346
ColumnRt-2560, 100 m, 0.25 mm ID, 0.20 µm (cat.# 13198)
SampleFood industry FAME mix (cat.# 35077)
Diluent:Hexane
Conc.:10,000 µg/mL total concentration
Injection
Inj. Vol.:1 µL split (split ratio 20:1)
Liner:Topaz 4.0 mm ID Precision inlet liner w/wool (cat.# 23305)
Inj. Temp.:250 °C
Oven
Oven Temp.:100 °C (hold 1.05 min) to 240 °C at 5.3 °C/min (hold 8.5 min)
Carrier GasH2, constant flow
Flow Rate:1.75 mL/min
DetectorFID @ 250 °C
Constant Column + Constant Make-up:52 mL/min
Hydrogen flow:40 mL/min
Air flow:400 mL/min
Data Rate:50 Hz
InstrumentAgilent 7890A GC
NotesResolution of all peaks >1.5
Method translated from He using the EZGC method translator (https://blog.restek.com/?p=52224).
Download PDF

RELATED CHROMATOGRAM LIBRARY SEARCHES

Fat

,

Oil

Printing tips! To print chromatograms full page, change the print settings in the print dialogue window of your PDF reader software: 1) "fit" or "fit to page", 2) "landscape" for wide chromatograms or "portrait" for tall chromatograms.


Restek Domestic Customer Service

Subject

Message

Your Full Name

Your Email

Company Name

Address

Spam Block (Please leave this blank)
Drag and Drop Attachments Here (optional)
...


all fields required

Thank you

Your message has been sent. We will be in touch shortly.

Message not sent

Sorry, your message could not be sent at this time. Please try again later, or contact Restek or your local Restek representative via phone.

www.restek.com/Contact-Us