Food Industry FAMEs on Rt-2560 by AOCS Method Ce-1j-07 Using H2 (Optimized Method)
Food Industry FAMEs on Rt-2560 by AOCS Method Ce-1j-07 Using H2 (Optimized Method)
Peaks
tR (min)
Conc. (µg/mL)
Structural Nomenclature
1.
Methyl caproate
4.740
400
C6:0
2.
Methyl octanoate
4.953
400
C8:0
3.
Methyl decanoate
5.317
400
C10:0
4.
Methyl undecanoate
5.587
200
C11:0
5.
Methyl dodecanoate
5.939
400
C12:0
6.
Methyl tridecanoate
6.398
200
C13:0
7.
Methyl myristate
6.998
400
C14:0
8.
Methyl myristoleate
7.736
200
C14:1 (c9)
9.
Methyl pentadecanoate
7.781
200
C15:0
10.
Methyl pentadecenoate
8.734
200
C15:1 (C10)
11.
Methyl palmitate
8.806
600
C16:0
12.
Methyl palmitoleate
9.831
200
C16:1 (c9)
13.
Methyl heptadecanoate
10.136
200
C17:0
14.
Methyl heptadecenoate
11.448
200
C17:1 (c10)
15.
Methyl stearate
11.882
400
C18:0
16.
Methyl octadecenoate
12.877
200
C18:1 (t9)
17.
Methyl oleate
13.345
400
C18:1 (c9)
18.
Methyl linolelaidate
14.685
200
C18:2 (t9,t12)
19.
Methyl linoleate
15.928
200
C18:2 (c9,c12)
20.
Methyl arachidate
17.136
400
C20:0
21.
Methyl linolenate
18.252
200
C18:3 (c6,c9,c12)
22.
Methyl eicosenoate
19.422
200
C20:1 (c11)
23.
Methyl linolenate
19.740
200
C18:3 (c9,c12,c15)
24.
Methyl heneicosanoate
20.592
200
C21:0
25.
Methyl eicosadienoate
21.621
200
C20:2 (c11,c14)
26.
Methyl behenate
22.380
400
C22:0
27.
Methyl eicosatrienoate
22.884
200
C20:3 (c8,c11,c14)
28.
Methyl erucate
23.510
200
C22:1 (c13)
29.
Methyl eicosatrienoate
23.656
200
C20:3 (c11,c14,c17)
30.
Methyl arachidonate
23.901
200
C20:4 (c5,c8,c11,c14)
31.
Methyl tricosanoate
24.240
200
C23:0
32.
Methyl docosadienoate
25.408
200
C22:2 (c13,c16)
33.
Methyl eicosapentaenoate
26.293
200
C20:5 (c5,c8,c11,c14,c17)
34.
Methyl lignocerate
26.293
400
C24:0
35.
Methyl nervonate
27.653
200
C24:1 (c15)
36.
Methyl docosahexaenoate
33.053
200
C22:6 (c4,c7,c10,c13,c16,c19)
GC_FF1348
Column
Rt-2560, 100 m, 0.25 mm ID, 0.20 µm (cat.# 13198)
Sample
Food industry FAME mix (cat.# 35077)
Diluent:
Hexane
Conc.:
10,000 µg/mL total concentration
Injection
Inj. Vol.:
1 µL split (split ratio 20:1)
Liner:
Topaz 4.0 mm ID Precision inlet liner w/wool (cat.# 23305)
Inj. Temp.:
250 °C
Oven
Oven Temp.:
180 °C (hold 19.8 min) to 215 °C at 32.3 °C/min (hold 11 min)
Carrier Gas
H2, constant flow
Flow Rate:
2.5 mL/min
Detector
FID @ 250 °C
Constant Column + Constant Make-up:
52 mL/min
Hydrogen flow:
40 mL/min
Air flow:
400 mL/min
Data Rate:
50 Hz
Instrument
Agilent 7890A GC
Notes
Method optimized from He using the EZGC method translator (https://blog.restek.com/?p=52224). C4:0 Methyl butyrate (623-42-7) elutes in the solvent front. Resolution of critical pair cis-11-C20:1 (#22) and cis-9,12,15-C18:3 (#23) is 2.03.
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