Pure Chromatography
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Water Soluble Vitamins on Ultra Aqueous C18
Peaks | Conc. (mg/mL) | |
---|---|---|
1. | Thiamin (B1)![]() | 250 |
2. | Ascorbic acid (C)![]() | 1,000 |
3. | Unknown | n/a |
4. | Nicotinic acid (B3)![]() | 1,000 |
Peaks | Conc. (mg/mL) | |
---|---|---|
5. | Unknown | n/a |
6. | Pantothenic acid (B5) | 1,000 |
7. | Folic acid (B9)![]() | 500 |
8. | Riboflavin (B2)![]() | 250 |
9. | Methylparaben![]() | 0.2 |

LC_0141
Column | Ultra Aqueous C18 (cat.# 9178575) | ||||||||||
Dimensions: | 250 mm x 4.6 mm ID | ||||||||||
Particle Size: | 5 µm | ||||||||||
Pore Size: | 100 Å | ||||||||||
Temp.: | 27 °C | ||||||||||
Sample | water soluble vitamins | ||||||||||
Diluent: | initial dilutions of B1 and B2 basified with ammonium hydroxide | ||||||||||
Conc.: | variable | ||||||||||
Mobile Phase | |||||||||||
A: | 25 mM potassium phosphate, pH 2.00: methanol (95:5) | ||||||||||
B: | methanol: 25 mM potassium phosphate, pH 3.5 (60:40) | ||||||||||
| |||||||||||
Flow: | 1 mL/min | ||||||||||
Detector | UV/Vis @ 254 nm |
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