Pure Chromatography
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Water Soluble Vitamins on Ultra Aqueous C18
| Peaks | Conc. (mg/mL) | |
|---|---|---|
| 1. | Thiamin (B1) | 250 |
| 2. | Ascorbic acid (C) | 1,000 |
| 3. | Unknown | n/a |
| 4. | Nicotinic acid (B3) | 1,000 |
| Peaks | Conc. (mg/mL) | |
|---|---|---|
| 5. | Unknown | n/a |
| 6. | Pantothenic acid (B5) | 1,000 |
| 7. | Folic acid (B9) | 500 |
| 8. | Riboflavin (B2) | 250 |
| 9. | Methylparaben | 0.2 |
LC_0141
| Column | Ultra Aqueous C18 (cat.# 9178575) | ||||||||||
| Dimensions: | 250 mm x 4.6 mm ID | ||||||||||
| Particle Size: | 5 µm | ||||||||||
| Pore Size: | 100 Å | ||||||||||
| Temp.: | 27 °C | ||||||||||
| Sample | water soluble vitamins | ||||||||||
| Diluent: | initial dilutions of B1 and B2 basified with ammonium hydroxide | ||||||||||
| Conc.: | variable | ||||||||||
| Mobile Phase | |||||||||||
| A: | 25 mM potassium phosphate, pH 2.00: methanol (95:5) | ||||||||||
| B: | methanol: 25 mM potassium phosphate, pH 3.5 (60:40) | ||||||||||
| |||||||||||
| Flow: | 1 mL/min | ||||||||||
| Detector | UV/Vis @ 254 nm |
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Restek Corporation, U.S., 110 Benner Circle, Bellefonte, PA 16823
Copyright © 2019 Restek Corporation. All rights reserved.
A Company of Owners
Cookies allow for a variety of features that make your visit to Restek more enjoyable. By using this website, you agree to the use of cookies in accordance with our guidelines. For more information, see our privacy policy.