Pure Chromatography
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25 ng/mL Benzodiazepines in Whole Blood on Ultra Biphenyl by LC/MS/MS
| Peaks | tR (min) | MRM1 | MRM2 | MRM3 | |
|---|---|---|---|---|---|
| 1. | 7-Aminoclonazepam | 0.57 | 286.0/250.3 | 286.0/222.2 | 286.0/121.0 |
| 2. | 7-Aminoflunitrazepam | 0.74 | 284.2/135.2 | 284.2/240.2 | 284.2/226.1 |
| 3. | Bromazepam | 1.07 | 315.9/182.0 | 315.9/209.1 | 315.9/260.9 |
| 4. | N-Desmethylflunitrazepam | 1.70 | 300.0/254.1 | 300.0/225.2 | 300.0/198.0 |
| 5. | Nitrazepam | 1.68 | 282.1/236.1 | 282.1/207.2 | 282.1/180.0 |
| 6. | Lorazepam | 1.70 | 321.0/302.9 | 321.0/275.1 | 321.0/229.1 |
| 7. | 2-Hydroxyethylflurazepam | 1.78 | 333.1/246.1 | 333.1/166.0 | 333.1/109.1 |
| 8. | Nordiazepam | 1.76 | 271.0/140.0 | 271.0/208.0 | 271.0/225.9 |
| 9. | Estazolam | 1.86 | 294.9/267.1 | 294.9/241.0 | 294.9/138.0 |
| 10. | Desalkylflurazepam | 1.90 | 289.0/140.0 | 289.0/165.1 | 289.0/226.0 |
| 11. | Clonazepam | 1.91 | 315.9/270.2 | 315.9/214.1 | 315.9/207.1 |
| 12. | Alprazolam | 1.98 | 309.4/281.1 | 309.4/205.0 | 309.4/274.1 |
| 13. | Midazolam | 1.22 | 326.1/291.2 | 326.1/244.2 | 326.1/209.0 |
| 14. | Triazolam | 2.05 | 343.1/315.0 | 343.1/308.2 | 343.1/239.2 |
| 15. | Temazepam | 2.12 | 301.2/283.1 | 301.2/255.1 | 301.2/228.1 |
| 16. | Flurazepam | 1.30 | 388.1/315.1 | 388.1/288.2 | 388.1/317.1 |
| 17. | Flunitrazepam | 2.23 | 314.4/211.0 | 314.4/239.1 | 314.4/268.1 |
| 18. | Clobazam | 2.26 | 301.1/259.1 | 301.1/224.0 | 301.1/181.1 |
| 19. | Diazepam-D5 (IS) | 2.40 | 290.0/154.1 | -- | -- |
| 20. | Diazepam | 2.44 | 284.8/193.1 | 284.8/222.2 | 284.8/257.1 |
LC_CF0522
| Column | Ultra Biphenyl (cat.# 9109552) | ||||||||||||||||||||||||||||
| Dimensions: | 50 mm x 2.1 mm ID | ||||||||||||||||||||||||||||
| Particle Size: | 5 µm | ||||||||||||||||||||||||||||
| Pore Size: | 100 Å | ||||||||||||||||||||||||||||
| Temp.: | 40 °C | ||||||||||||||||||||||||||||
| Sample | |||||||||||||||||||||||||||||
| Diluent: | Starting mobile phase + 30 ng/mL IS | ||||||||||||||||||||||||||||
| Conc.: | 25 ng/mL sample was diluted 20x prior to injection | ||||||||||||||||||||||||||||
| Inj. Vol.: | 30 µL | ||||||||||||||||||||||||||||
| Mobile Phase | |||||||||||||||||||||||||||||
| A: | Water + 2 mM ammonium formate + 0.2% formic acid | ||||||||||||||||||||||||||||
| B: | Acetonitrile + 10% water + 2 mM ammonium formate + 0.2% formic acid | ||||||||||||||||||||||||||||
| |||||||||||||||||||||||||||||
| Detector | AB SCIEX API 4000 MS/MS | ||||||||||||||||||||||||||||
| Model #: | API 4000 | ||||||||||||||||||||||||||||
| Ion Source: | TurboIonSpray® | ||||||||||||||||||||||||||||
| Ion Mode: | ESI+ | ||||||||||||||||||||||||||||
| Ion Spray Voltage: | 2 kV | ||||||||||||||||||||||||||||
| Curtain Gas: | 40 psi (275.8 kPa) | ||||||||||||||||||||||||||||
| Gas 1: | 40 psi (275.8 kPa) | ||||||||||||||||||||||||||||
| Gas 2: | 40 psi (275.8 kPa) | ||||||||||||||||||||||||||||
| Source Temp.: | 600 °C | ||||||||||||||||||||||||||||
| Mode: | MRM | ||||||||||||||||||||||||||||
| Instrument | Applied Biosystems/MDS Sciex LC-MS/MS System | ||||||||||||||||||||||||||||
| Notes | MS/MS instrument was operated in scheduled MRM mode. |
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Copyright © 2021 Restek Corporation. All rights reserved.
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Cookies allow for a variety of features that make your visit to Restek more enjoyable. By using this website, you agree to the use of cookies in accordance with our guidelines. For more information, see our privacy policy.