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Norbuprenorphine & Buprenorphine in Urine on Raptor Biphenyl by LC-MS/MS

Norbuprenorphine & Buprenorphine in Urine on Raptor Biphenyl by LC-MS/MS

PeakstR (min)Parent IonProduct Ion 1Product Ion 2
1.Norbuprenorphine0.63414.183.4101.0
2.Buprenorphine0.84468.3396.4414.5
image
LC_CF0580
ColumnRaptor Biphenyl (cat.# 9309A5E)
Dimensions:50 mm x 3.0 mm ID
Particle Size:2.7 µm
Temp.:30 °C
Sample
Conc.: 100 ng/mL standard was prepared in 1 mL urine. The 1 mL urine standard was then diluted with 2 mL of 0.1% formic acid in methanol:water (50:50) prior to analysis.
Inj. Vol.:10 µL
Mobile Phase
A:0.1% Formic acid in water
B:0.1% Formic acid in methanol
Time (min)Flow (mL/min)%A%B
0.000.754555
1.00.750100
1.50.750100
1.510.754555
2.00.754555
DetectorAB SCIEX API 4000 MS/MS
Ion Source:TurboIonSpray®
Ion Mode:ESI+
InstrumentAPI LC-MS/MS
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Norbuprenorphine

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buprenorphine

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Raptor

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Biphenyl

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